Core C: Myeloid cell and atherosclerosis core

“甘油三酯、糖尿病和动脉粥样硬化核心(核心C)”项目中的髓细胞和动脉粥样硬化核心(核心C) 心血管疾病“将为个别项目提供分析技术，以彻底分析 来自不同小鼠模型的动脉粥样硬化病变的程序内使用。这些分析将 包括组织学染色方法、病变大小测量、病变形态特征 免疫组织化学和激光捕获显微切割技术进行基因表达分析。核心也将 提供RNA测序和单细胞RNA测序数据的专业知识和生物信息学分析 对项目调查员的项目进行补充。这一核心的高质量髓系分析曲目 计划项目中研究的细胞、动脉粥样硬化和其他组织将大大增强 四个单项项目的科学进步。

The Myeloid Cell and Atherosclerosis Core (Core C) within the Program Project “Triglycerides, Diabetes and Cardiovascular Disease” will provide individual projects with analytical techniques for thorough analysis of lesions of atherosclerosis from the different mouse models used within the program. These analyses will include histological staining methods, lesion size measurements, lesion morphology characterization, immunohistochemistry, and laser capture microdissection for analysis of gene expression. The core will also provide expertise and bioinformatics analysis of RNA-sequencing and single cell RNA-sequencing data as a complement to the Program Investigators’ projects. This Core’s repertoire of high-quality analyses of myeloid cells, atherosclerosis and other tissues studied within the Program Project will substantially enhance the scientific progress of the four individual projects.