Investigating Ventral CA1 Neural Representations of Anxiety-Like Behavior

研究焦虑样行为的腹侧 CA1 神经表征

• 批准号: 10643862
• 负责人: Sean Chih-Hsiung Lim
• 金额: $ 5.27万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10643862
• 关键词: Address; Algorithms; Amygdaloid structure; Animals; Anti-Anxiety Agents; Anxiety; Anxiety Disorders; Aversive Stimulus; Award; Behavioral; Biological Assay; Brain; Brain region; Calcium; Cells; Characteristics; Code; Data; Dependovirus; Detection; Dimensions; Emotional; Environment; Feeling; Fellowship; Goals; Head; Hippocampus; Image; Knowledge; Lesion; Mediating; Methods; Mus; National Research Service Awards; Neurons; Neurosciences; Output; Pathologic; Pattern; Pharmaceutical Preparations; Physicians; Population; Population Analysis; Posture; Psychiatry; Research; Role; Scientist; Stretching; System; Testing; Therapeutic; Time; Visualization; anxiety reduction; anxiety states; anxiety-like behavior; avoidance behavior; behavioral response; computational neuroscience; emotional behavior; in vivo calcium imaging; insight; machine learning method; microendoscope; negative emotional state; neural; neural circuit; neurodevelopment; novel; optogenetics; research and development; unsupervised learning

PROJECT SUMMARY The ability to detect and appropriately respond to potentially threatening situations is critical for survival. Dysregulation of the neural systems which mediate these functions can lead to pathological avoidance of ordinary environments and persistent feelings of imminent threat, key components of the negative emotional state characteristic of anxiety disorders. Many studies have begun to reveal the brain regions and neural circuits that control avoidance behavior and encode states of anxiety. Specifically, the ventral hippocampus has emerged as a critical node for emotional behaviors including those evoked by anxiogenic environments. Our lab and others have found that subpopulations of single neurons in ventral CA1 are activated by aversive environments, but this selectivity does not generalize across different tasks used to assay anxiety-like behavior. Moreover, it is unclear which inputs to the ventral hippocampus provide the emotional information that drives this anxiety-related neural activity. The mechanism by which the ventral hippocampus encodes anxiety states is still unknown and investigating how this brain region represents these emotional states is an essential step towards understanding the neural substrates of anxiety disorders. In this proposal, I will utilize pose estimation algorithms and unsupervised behavioral segmentation (Aim 1), in vivo calcium imaging and neural population analyses (Aim 2), along with simultaneous input-specific optogenetic inhibition and calcium imaging (Aim 3) to characterize the behavioral manifestations of anxiety-like emotional states and determine how they are encoded in the ventral hippocampus. Successful completion of this study will help to elucidate the role of the ventral hippocampus in anxiety, potentially opening new research avenues for the development of neural circuit based therapeutics to treat anxiety disorders. These data along with the research and scientific expertise developed through this Kirschstein-NRSA F30 Fellowship Award will support my long-term goal of becoming an independent physician-scientist.

项目总结