Molecular epidemiology of respiratory virus exposure in elementary schools

小学呼吸道病毒暴露的分子流行病学

• 批准号: 10646577
• 负责人: Peggy Sue Lai
• 金额: $ 26.79万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-10 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10646577
• 关键词: Adenoviruses; Adherence; Affect; Air; Asthma; Behavior; COVID-19; COVID-19 outbreak; COVID-19 patient; Carbon Dioxide; Child; Childhood; Contact Tracing; Coronavirus; Data; Detection; Disease Outbreaks; Disinfectants; Dust; Enterovirus; Environment; Environmental Health; Environmental Risk Factor; Exposure to; Fatigue; Future; Genetic; Genome; Goals; Handwashing; Home; Hospitalization; Household; Human; Humidity; Hybrids; Incidence; Individual; Indoor environment; Influenza A virus; Infrastructure; Intervention; Intervention Studies; Mandatory Reporting; Masks; Measles; Metadata; Metapneumovirus; Methods; Mission; Molecular Epidemiology; Morbidity - disease rate; Morbillivirus; Mumps virus; Parainfluenza; Particulate; Pathogenicity; Placebo Control; Predictive Factor; Randomized, Controlled Trials; Research; Respiratory syncytial virus; Rhinovirus; Risk; Rubella virus; SARS coronavirus; Sampling; Schools; Source; Spain; Students; Surface; Symptoms; Temperature; Testing; Time; United States; United States National Institutes of Health; Vaccines; Viral; Viral Genome; Viral Respiratory Tract Infection; Work; air filtration; built environment; design; disease transmission; effective intervention; elementary school; environmental intervention; evidence base; high efficiency particulate air filter; high risk; influenzavirus; inner city; innovation; metagenomic sequencing; next generation sequencing; novel strategies; pandemic disease; pandemic influenza; pathogen; portability; protective behavior; public health intervention; randomized placebo controlled trial; reconstruction; respiratory; respiratory infection virus; respiratory virus; sample collection; school environment; ventilation; virome

PROJECT SUMMARY/ABSTRACT There is a fundamental gap in understanding the extent to which the school environment contributes to respiratory virus exposure, and less is known about potential environmental interventions to reduce exposure. Our long-term goal is to create healthy indoor school environments for children. The overall objective of this application is to identify modifiable factors of respiratory virus exposure in elementary schools in order to reduce asthma morbidity. We will combine novel approaches to quantify and characterize the respiratory virome in schools by leveraging the robust infrastructure of the School Inner-City Asthma Intervention Study (SICAS-2, ClinicalTrials.gov NCT02291302), a randomized controlled trial conducted by our group of a placebo-controlled classroom high efficiency particulate air (HEPA) cleaner intervention. We will test our central hypothesis that schools are a source of high respiratory virus exposure that affects asthma morbidity through the following specific aims: (1) To compare respiratory virus exposure in schools as compared to homes by using a hybrid capture panel to recover genomes of detected respiratory viruses; (2) To identify factors predicting respiratory virus exposure in schools including the efficacy of portable HEPA cleaners in reducing classroom respiratory virus exposure; (3) To determine the association between the classroom respiratory virome and asthma symptoms in elementary school children. The approach is innovative, because we are simultaneously interrogating all major human respiratory viruses in the school environment. The proposed research is significant, because it will identify the magnitude of exposure to respiratory viruses in schools and potential environmental factors that can serve as future targets of intervention. If the HEPA intervention can reduce respiratory virus exposure, it is an immediately actionable and practical intervention to create safer indoor environments for elementary school children. Results from this proposal may be generalizable to other public indoor settings.

项目概要/摘要 在理解学校环境对儿童的影响程度方面存在着根本性的差距 呼吸道病毒暴露，而对于减少暴露的潜在环境干预措施知之甚少。 我们的长期目标是为孩子们创造健康的室内学校环境。本次活动的总体目标 该应用程序旨在确定小学中呼吸道病毒暴露的可改变因素，以便 减少哮喘发病率。我们将结合新方法来量化和表征呼吸系统 利用学校内城哮喘干预研究的强大基础设施，在学校中传播病毒组 （SICAS-2，ClinicalTrials.gov NCT02291302），由我们小组进行的一项随机对照试验 安慰剂对照的教室高效颗粒空气 (HEPA) 清洁剂干预。我们将测试我们的 中心假设是学校是影响哮喘发病率的高呼吸道病毒暴露来源 通过以下具体目标： (1) 比较学校和学校的呼吸道病毒暴露情况 通过使用混合捕获面板来恢复检测到的呼吸道病毒的基因组； (2) 识别 预测学校呼吸道病毒暴露的因素，包括便携式 HEPA 清洁器的功效 减少课堂呼吸道病毒暴露； (3)确定课堂之间的关联性 小学生的呼吸道病毒组和哮喘症状。该方法具有创新性，因为 我们正在同时检测学校环境中所有主要的人类呼吸道病毒。这 拟议的研究意义重大，因为它将确定人们接触呼吸道病毒的程度 学校和潜在的环境因素可以作为未来的干预目标。如果高效空气过滤器 干预措施可以减少呼吸道病毒的暴露，这是一种立即可行且实际的干预措施 为小学生创造更安全的室内环境。该提案的结果可能是 可推广到其他公共室内环境。