Plasticity of cortical networks during learning

学习过程中皮质网络的可塑性

• 批准号: 10537052
• 负责人: Hannah Marie Batchelor
• 金额: $ 3.16万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2026-09-15
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10537052
• 关键词: Address; Animals; Automobile Driving; Behavior; Behavioral; Behavioral Paradigm; Calcium; Cues; Data; Disease; Dorsal; Event; Excision; Exhibits; Goals; Graph; Image; Imaging Techniques; Individual; Learning; Methods; Motivation; Motor; Motor Cortex; Mus; Neocortex; Neurons; Phase; Population; Process; Reversal Learning; Rewards; Role; Schizophrenia; Shapes; Site; Stimulus; Symptoms; Techniques; Testing; Time; Training; V1 neuron; Visual; Visual Cortex; Work; addiction; analytical tool; area striata; base; classical conditioning; design; experimental study; hippocampal pyramidal neuron; insight; novel; optogenetics; prevent; recruit; relating to nervous system; response; spatiotemporal; two-photon; visual processing

PROJECT SUMMARY: Plasticity occurs in the primary visual cortex (V1) in response to visual associative learning, where one stimulus is paired with reward (CS+) and another is not (CS-). However, learning is not a unitary process, and distinct stages of learning might drive contrasting plastic changes in V1. Indeed, previous work has found dynamic changes in V1 pyramidal neuron activity and inputs at different times throughout learning. Experiments separating distinct learning phases behaviorally are lacking, making it difficult to fully dissect V1 plasticity changes throughout learning. To address this, we have designed a 3-phase associative learning task that separates early, stimulus non-specific learning from late, stimulus-specific learning and reversal learning. We will also manipulate stimulus value in two ways: devaluation and spout removal. Using this behavioral paradigm along with 1- and 2-photon calcium imaging of the mouse dorsal cortex, we will test the following hypotheses: 1) that visual associative learning differentially recruits V1 activity during distinct learning stages, 2) activity in V1 is rapidly plastic in response to changes in stimulus value, 3) individual V1 neurons exhibit bidirectional plasticity across learning, 4) visual associative learning changes cortico-cortical functional connectivity, and 5) V1 activity is required to maintain these cortico-cortical functional connectivity changes. Our results will provide an unprecedented level of insight into V1 plasticity throughout learning and stimulus value manipulation. We will, for the first time, address how plasticity in V1 is mirrored across the dorsal cortex, or required for cortico-cortical plasticity.

项目总结： 可塑性发生在初级视觉皮质(V1)对视觉联想学习的反应中，其中一个 刺激与奖励配对(CS+)，另一个不配对(CS-)。然而，学习不是一个统一的过程，而且 不同的学习阶段可能会促使V1发生截然不同的塑性变化。事实上，之前的工作已经发现 在整个学习过程中不同时间V1锥体神经元活动和输入的动态变化。实验 在行为上缺乏区分不同的学习阶段，这使得完全剖析V1可塑性变得困难 在整个学习过程中会发生变化。为了解决这个问题，我们设计了一个三阶段联想学习任务， 将早期刺激性非特定学习与晚期刺激性特定学习和反转学习分开。我们 还将通过两种方式操纵刺激价值：贬值和去除喷嘴。使用这种行为范式 在对小鼠背侧皮质进行单光子和双光子钙成像的同时，我们将检验以下假设：1) 视觉联想学习在不同的学习阶段差异地招募V1活动，2)V1中的活动是 对刺激值变化的快速可塑性反应，3)单个V1神经元表现出双向可塑性 在整个学习过程中，4)视觉联想学习改变了皮质-皮质功能连接，以及5)V1活动 维持这些皮质-皮质功能连接变化所必需的。我们的结果将提供一个 在整个学习和刺激值操作中，对V1可塑性的洞察达到前所未有的水平。我们会的， 第一次，阐述V1的可塑性是如何在背侧皮质上反映出来的，或者是皮质所必需的 可塑性。