Structural Investigation of p120RasGAP's regulation by phosphorylated binding partners

磷酸化结合伴侣对 p120RasGAP 调节的结构研究

• 批准号: 10535097
• 负责人: Kimberly Jean Vish
• 金额: $ 4.68万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10535097
• 关键词: Affect; Affinity; Area; Arteries; Arteriovenous malformation; Binding; Binding Sites; Biochemical; Biological Assay; Biophysics; Blood; Blood Circulation; Blood Vessels; Blood capillaries; Brain; Calorimetry; Cell Extracts; Cells; Complex; Crystallization; Data; Development; Disease; Family; Fluorescence; GRLF1 gene; GTPase-Activating Proteins; Galen Vein; Guanosine Triphosphate; In Vitro; Investigation; Knowledge; Lead; Length; Maps; Measures; Molecular; Molecular Conformation; Mutation; N-terminal; Newborn Infant; Outcome; Pathogenicity; Pathway interactions; Patients; Peptides; Phosphopeptides; Phosphotyrosine; Proteins; RAS genes; Receptor Protein-Tyrosine Kinases; Regulation; Roentgen Rays; Signal Transduction; Structure; Syndrome; Tertiary Protein Structure; Testing; Titrations; Tyrosine; Vascular Diseases; Work; X-Ray Crystallography; base; disease phenotype; malformation; mutant; p120 GTPase Activating Protein; recruit; rho; rho GTPase-activating protein; src Homology Region 2 Domain

Project Summary/Abstract The EphB4-p120RasGAP signaling axis is critical for vascular development and mutations in both EphB4 and p120RasGAP are associated with vascular disorders including Vein of Galen Malformation (VOGM) and Capillary Malformation-Arteriovenous Malformation (CM-AVM) Syndrome. One key protein along this pathway, p120RasGAP (RasGAP), is particularly under studied. Despite RasGAP being the first GTPase Activating Protein (GAP) discovered it is still unclear how RasGAP is regulated. RasGAP contains two SH2 domains, allowing it to signal downstream of phosphotyrosine-containing proteins, such as the receptor tyrosine kinase EphB4 and the RhoGAP p190RhoGAP (p190). Multiple tyrosine phosphorylated binding partners of RasGAP elicit different signaling outcomes, and previous studies suggest these binding partners may influence RasGAP activity. However, the molecular basis for how this occurs is unknown. Uncovering how these binding partners regulate RasGAP’s activity paves the way to understand how mutations associated with VOGM and CM-AVM might disrupt this regulation. In this proposal I will conduct structural, biophysical, and enzymatic studies to test the hypothesis phosphotyrosine-containing binding partners regulate RasGAP’s signaling activity. Aim 1: To determine the effects of non-catalytic domains, binding partner interactions, and mutations on GAP regulation. Due to studies in cell extract and immunoprecipated protein, phosphorylated binding partners are hypothesized to influence RasGAP’s GAP activity. However, there have been no studies performed using purified proteins to assess this hypothesis. In Aim 1 I propose in purified protein fluorescent GAP assays to determine how RasGAP’s non-catalytic domains, binding partners, and disease mutations influence GAP activity. Aim 2: To determine conformational changes in RasGAP upon different binding partner interactions. Despite the importance of the EphB4-RasGAP interaction it is still unknown how these proteins interact. A structure determined in the Boggon Lab of RasGAP’s N-terminal domains with a doubly phosphorylated p190 peptide show it is impossible for EphB4’s phosphotyrosine residues to engage RasGAP the same way. Therefore, in Aim 2 I will determine how EphB4 engages RasGAP and determine the conformational effects on RasGAP. I propose X-ray crystallography, Small Angle X-ray Scattering, and Isothermal Titration Calorimetry to probe the conformational changes induced in RasGAP by p190 and EphB4 binding. I will then assess the impact of disease-associated mutations in the context of these biophysical data and probe the impact of disease- associated mutations on conformation, structure, and activity.

项目总结/文摘