Rodent Phenotyping Core

啮齿动物表型核心

• 批准号: 10663391
• 负责人: Michael G Anderson
• 金额: $ 17.98万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10663391
• 关键词: Acute; Address; Animal Husbandry; Anterior; Area; Axon; Biological; Biological Assay; COVID-19; Categories; Collaborations; Consult; Consultations; Development; Dissection; Educational workshop; Electroretinography; Experimental Designs; Eye diseases; Fees; Fluorescein Angiography; Funding; Fundus; Goals; Gonioscopy; Housing; Human Resources; Injections; Investigation; Iowa; Light; Lighting; Measures; Mission; Modeling; Mus; Optic Nerve; Phenotype; Photography; Physiologic Intraocular Pressure; Procedures; Pupil light reflex; Rattus; Reproducibility; Research Personnel; Resources; Retina; Retinal Ganglion Cells; Rodent; Science; Services; Slide; System; Temperature; Testing; Tissues; Training; United States National Institutes of Health; Universities; Vision; Vision Tests; Vision research; automated image analysis; behavior measurement; deep learning; equipment acquisition; experimental study; investigator training; model organism; multidisciplinary; pandemic disease; response; sperm cryopreservation; vision science

PROJECT SUMMARY The goal of the Rodent Phenotyping Core (RPC) is to increase the utility of mice and rats as model organisms for vision research at The University of Iowa. The availability of a centralized core for phenotyping will allow investigators to focus their resources on the science and discoveries underlying vision research, rather than on training each lab’s personnel to perform phenotyping. Such core-based services will yield more consistent and reproducible phenotyping measures and in turn maximize the power of NEI-funded investigators to make new discoveries. The RPC will have the capability to perform detailed phenotyping of the anterior segment (slit- lamp examination and photography, SD-OCT, gonioscopy, fluorescein angiography, and intraocular pressure recording) and the posterior segment (fundus examination/photography, electroretinogram, pupil light reflex, behavioral measurements of vision, acute suppression of activity by light, and testing of optokinetic response).

项目摘要 啮齿动物表型核心（RPC）的目标是增加小鼠和大鼠的效用， 爱荷华州大学视觉研究的模式生物。一个集中的可用性 表型分析的核心将使研究人员能够将资源集中在科学上， 视觉研究的基础发现，而不是训练每个实验室的人员执行 表型分析这种基于核心的服务将产生更一致和可复制的结果 表型的措施，反过来最大限度地发挥权力NEI资助的研究人员，使 新发现 RPC将有能力对眼前节（裂隙）进行详细的表型分析。 灯检查和摄影，SD-OCT，房角镜检查，荧光素血管造影，和 眼内压记录）和后段（眼底检查/摄影， 视网膜电图，瞳孔对光反射，视觉行为测量，急性抑制 通过光的活性和视动反应的测试）。