Targets and functions of the mammalian snoRNAome
哺乳动物 snoRNAome 的目标和功能
基本信息
- 批准号:10565187
- 负责人:
- 金额:$ 77.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-22 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAntisense OligonucleotidesBindingBinding SitesBiological ProcessBiologyCell LineCellsComplexGene ExpressionGene Expression RegulationGenesGenetic TranslationGuide RNAHumanHuman GenomeIndividualInvestigationKnowledgeMapsMessenger RNAMethodsModificationMusMutationNucleotidesOrphanPathway interactionsProteinsPseudouridineRNARNA BindingRNA SplicingRNA-Binding ProteinsRegulationResearchResolutionRibonucleoproteinsRibosomal RNARibosomesRoleSmall Nucleolar RNASmall Nucleolar RibonucleoproteinsStressTechnologyTimeTissuesTranslationsUntranslated RNAbasebiological systemsbisulfitecell typehuman diseaseinventionkethoxalknock-downmRNA PrecursormRNA Stabilitynanoporenovel sequencing technologynovel strategiesprotein complexresponsetooltranscriptometranscriptomics
项目摘要
Project Abstract:
Small nucleolar RNA (snoRNA) are natural guide non-coding RNAs derived from over 900 annotated snoRNA
genes in the human genome. The major known function of about one-third of snoRNAs is to install 2’O-methyl
and pseudouridine modifications in the ribosomal RNA. These rRNA modifications guide ribosome assembly
and maturation, and fine-tune translation in a cell type and cell state dependent manner. However, the majority
of snoRNAs do not have a well described function; they are called orphans because their cellular RNA targets
are not known. How snoRNA interacts with the human transcriptome and the functional consequences of these
potential non-canonical snoRNA-RNA interactions in gene expression regulation and human diseases remain to
be determined. We recently developed several new sequencing technologies that are directly relevant to
studying snoRNA biology. They include methods to robustly identify inter-molecular RNA-RNA interactions in
cells and transcriptome-wide sequencing of 2’O-methyl and pseudouridine modifications. These new tools will
allow us to address mysteries of snoRNA biology. Aim 1 will identify the cellular RNA targets of the snoRNAome
through an advanced version of kethoxal-assisted RNA-RNA interaction sequencing (KARR-seq) approach to
enable comprehensive capture and identification of snoRNA-RNA interactions at the transcriptomic scale. These
results will be used to identify the rules of snoRNA-guided targeting of mRNA sequences. Aim 2 will investigate
two types of snoRNA-mRNA interactions transcriptome-wide and the consequences on regulating gene
expression through the application of new sequencing methods to map 2’O-methyl and pseudouridine
modifications in pre-mRNA and mature mRNA, and associate these results with the snoRNA-mRNA interactome.
We will also selectively delete snoRNA genes or knockdown snoRNA levels, alter the expression of snoRNP
components to investigate the consequence of specific snoRNA-mRNA interaction. All together these results will
provide the first comprehensive snoRNA-RNA interactome and derive the guiding principles of snoRNA-mRNA
interactions and the associated biological functions.
项目简介:
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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{{ truncateString('CHUAN HE', 18)}}的其他基金
Targets and functions of the mammalian snoRNAome
哺乳动物 snoRNAome 的目标和功能
- 批准号:
10708950 - 财政年份:2022
- 资助金额:
$ 77.38万 - 项目类别:
Mechanosensitive M7G epitranscriptome in endothelial health and disease
机械敏感 M7G 表观转录组在内皮健康和疾病中的作用
- 批准号:
10367181 - 财政年份:2021
- 资助金额:
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Mechanosensitive M7G epitranscriptome in endothelial health and disease
机械敏感 M7G 表观转录组在内皮健康和疾病中的作用
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10543139 - 财政年份:2021
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- 资助金额:
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10434878 - 财政年份:2016
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Center for dynamic RNA epitranscriptomes - Renewal
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- 批准号:
9975937 - 财政年份:2016
- 资助金额:
$ 77.38万 - 项目类别:
Center for dynamic RNA epitranscriptomes - Renewal
动态 RNA 表观转录组中心 - 更新
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- 资助金额:
$ 77.38万 - 项目类别:
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10163382 - 财政年份:2016
- 资助金额:
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