Targets and functions of the mammalian snoRNAome

哺乳动物 snoRNAome 的目标和功能

基本信息

  • 批准号:
    10565187
  • 负责人:
  • 金额:
    $ 77.38万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2022
  • 资助国家:
    美国
  • 起止时间:
    2022-09-22 至 2026-06-30
  • 项目状态:
    未结题

项目摘要

Project Abstract: Small nucleolar RNA (snoRNA) are natural guide non-coding RNAs derived from over 900 annotated snoRNA genes in the human genome. The major known function of about one-third of snoRNAs is to install 2’O-methyl and pseudouridine modifications in the ribosomal RNA. These rRNA modifications guide ribosome assembly and maturation, and fine-tune translation in a cell type and cell state dependent manner. However, the majority of snoRNAs do not have a well described function; they are called orphans because their cellular RNA targets are not known. How snoRNA interacts with the human transcriptome and the functional consequences of these potential non-canonical snoRNA-RNA interactions in gene expression regulation and human diseases remain to be determined. We recently developed several new sequencing technologies that are directly relevant to studying snoRNA biology. They include methods to robustly identify inter-molecular RNA-RNA interactions in cells and transcriptome-wide sequencing of 2’O-methyl and pseudouridine modifications. These new tools will allow us to address mysteries of snoRNA biology. Aim 1 will identify the cellular RNA targets of the snoRNAome through an advanced version of kethoxal-assisted RNA-RNA interaction sequencing (KARR-seq) approach to enable comprehensive capture and identification of snoRNA-RNA interactions at the transcriptomic scale. These results will be used to identify the rules of snoRNA-guided targeting of mRNA sequences. Aim 2 will investigate two types of snoRNA-mRNA interactions transcriptome-wide and the consequences on regulating gene expression through the application of new sequencing methods to map 2’O-methyl and pseudouridine modifications in pre-mRNA and mature mRNA, and associate these results with the snoRNA-mRNA interactome. We will also selectively delete snoRNA genes or knockdown snoRNA levels, alter the expression of snoRNP components to investigate the consequence of specific snoRNA-mRNA interaction. All together these results will provide the first comprehensive snoRNA-RNA interactome and derive the guiding principles of snoRNA-mRNA interactions and the associated biological functions.
项目简介:

项目成果

期刊论文数量(0)
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会议论文数量(0)
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CHUAN HE的其他文献

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{{ truncateString('CHUAN HE', 18)}}的其他基金

Targets and functions of the mammalian snoRNAome
哺乳动物 snoRNAome 的目标和功能
  • 批准号:
    10708950
  • 财政年份:
    2022
  • 资助金额:
    $ 77.38万
  • 项目类别:
Mechanosensitive M7G epitranscriptome in endothelial health and disease
机械敏感 M7G 表观转录组在内皮健康和疾病中的作用
  • 批准号:
    10367181
  • 财政年份:
    2021
  • 资助金额:
    $ 77.38万
  • 项目类别:
Mechanosensitive M7G epitranscriptome in endothelial health and disease
机械敏感 M7G 表观转录组在内皮健康和疾病中的作用
  • 批准号:
    10543139
  • 财政年份:
    2021
  • 资助金额:
    $ 77.38万
  • 项目类别:
Interrogation of dynamic RNA modifications in beta cells in type 1 diabetes
1 型糖尿病 β 细胞动态 RNA 修饰的研究
  • 批准号:
    9459617
  • 财政年份:
    2017
  • 资助金额:
    $ 77.38万
  • 项目类别:
Center for dynamic RNA epitranscriptomes - Renewal
动态 RNA 表观转录组中心 - 更新
  • 批准号:
    10434878
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:
Center for dynamic RNA epitranscriptomes - Renewal
动态 RNA 表观转录组中心 - 更新
  • 批准号:
    10159493
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:
Mapping the epigenome and transcriptome during hippocampal neurogenesis
绘制海马神经发生过程中的表观基因组和转录组图谱
  • 批准号:
    9975937
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:
Center for dynamic RNA epitranscriptomes - Renewal
动态 RNA 表观转录组中心 - 更新
  • 批准号:
    10615783
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:
Center for dynamic RNA epitranscriptomes
动态RNA表观转录组中心
  • 批准号:
    9070962
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:
Center for dynamic RNA epitranscriptomes - Covid 19 Supplement Version 2
动态 RNA 表观转录组中心 - Covid 19 补充版本 2
  • 批准号:
    10163382
  • 财政年份:
    2016
  • 资助金额:
    $ 77.38万
  • 项目类别:

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开发反义寡核苷酸来调节γ纤维蛋白原水平
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用反义寡核苷酸诱导 PKM 剪接转换作为治疗肝细胞癌的方法
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  • 财政年份:
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  • 资助金额:
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