Core B -- BioCore

核心B——BioCore

• 批准号: 10559174
• 负责人: CHRISTOPHER L COE
• 金额: $ 63.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-25 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10559174
• 关键词: Aliquot; Alzheimer' s disease related dementia; Alzheimer' s disease risk; Antioxidants; Archives; Biological; Biological Assay; Biological Markers; Blood; Blood Tests; Body Fluids; Catecholamines; Cell Separation; Cells; Clinical; Code; Collection; Consult; Consultations; Data; Data Files; Development; Education; Educational workshop; Endocrine; Ensure; Fluorescence; Freezing; Future; Gene Expression; Genetic Transcription; Health; Hematology; High Density Lipoproteins; Home; Hormones; Hydrocortisone; Immunoassay; Immunology procedure; Inflammatory; Information Dissemination; Infrastructure; Institutional Review Boards; Laboratories; Link; Liquid substance; Location; Mass Spectrum Analysis; Measures; Mentors; Metabolic; Methodology; Methods; Mission; Monitor; Nitrogen; Paper; Participant; Pathway interactions; Performance; Peroxidases; Preparation; Process; Protocols documentation; Public Domains; Publications; Quality Control; Records; Reporting; Research Personnel; Resources; Risk; Role; Saliva; Salivary; Sampling; Ships; Site; Specimen; Spottings; Standardization; Sum; System; Test Result; Testing; Time; Workload; biological specimen archives; biomarker panel; bone turnover; chemokine; cytokine; data sharing; diaries; experience; liquid chromatography mass spectrometry; meetings; operation; pedagogy; psychosocial; quality assurance; repository; transmission process; urinary

ABSTRACT – BIOCORE To obtain the full panel of biomarkers in the MIDUS project, collection and analytic efforts must be coordinated. The BioCore (B) thus fulfills an essential role in MIDUS by providing centralized, reliable testing, processing, and storage of biospecimens (i.e., hematological, endocrine, inflammatory, metabolic, bone turnover, antioxidant, gene expression). All tests are performed by single laboratories to ensure consistent results over time. Core B also assists in the standardization of protocols (i.e., identical collection and processing SOPs) and monitors shipment integrity and testing at the analytic laboratories over time (i.e., Quality Control). Core B also has the primary responsibility for storing aliquots from new samples and maintaining a complete set of archival specimens in ultracold freezers. In addition to overseeing all testing, the BioCore conducts assays and transports the samples that are tested at local clinical labs in Madison, WI. A centralized Core operation is essential to ensure uniform entry of test results into a single record system. One corollary of this administrative function is reviewing the reliability of data entry before transmission of results for dissemination and combining with demographic/psychosocial data. Core B also has several advisory and pedagogical functions, which includes providing “norms” for test results, meeting with investigators to advise on analyses, and conducting educational workshops on the use and meaning of biological measures at MIDUS meetings. The future performance of the BioCore will be facilitated by the extensive experience gained during the prior MIDUS periods. The panel of biomarkers going forward is similar to the panels employed previously with some new additions. The BioCore has previously assisted in establishing new methodologies (e.g., multi-cytokine arrays, an assay of HDL anti-oxidant activity) and will also oversee new analyte testing related to risk for Alzheimer’s Disease and Related Dementias (ADRD) linked with the Ancillary U01 application. To highlight the BioCore’s level of experience, over 100,000 specimens have been tested in the prior periods. Processing of blood and/or cells for gene expression is also routine for Dr. Coe’s (PI, BioCore) laboratory. Dr. Coe has over 3 decades of prior experience with endocrine and immune assays, including with many body fluids, different assay platforms and cell separation protocols. In sum, Core B provides the infrastructure and required resources to ensure high quality and reliable biomarker assessments in MIDUS.

摘要- BIOCORE 为了在MIDUS项目中获得完整的生物标志物，必须协调收集和分析工作。 因此，BioCore（B）通过提供集中、可靠的测试、处理 以及生物样本的存储（即，血液学、内分泌、炎症、代谢、骨转换， 抗氧化剂、基因表达）。所有测试均由单个实验室进行，以确保结果一致。 时间核心B还有助于协议的标准化（即，相同的采集和处理SOP）， 监控运输完整性和分析实验室随时间的测试（即，质量控制）。核心B还 主要负责储存新样本的等分试样并维护一套完整的档案 超低温冷冻箱中的标本。除了监督所有测试外，BioCore还进行分析， 运输在麦迪逊，WI的当地临床实验室进行检测的样本。集中的核心运营是 这对于确保将测试结果统一输入单一记录系统至关重要。这种行政管理的一个必然结果是， 一项职能是在传输结果以便传播和合并之前审查数据输入的可靠性 人口统计学/心理社会学数据。核心B还具有若干咨询和教学功能， 包括提供测试结果的“规范”，与研究人员会面，就分析提供建议， 在MIDUS会议上举办关于生物措施的使用和意义的教育讲习班。未来 BioCore的性能将通过在先前的MIDUS期间获得的丰富经验来促进 时期未来的生物标志物小组类似于以前使用的小组， 的增订条文生物核心以前曾协助建立新的方法（例如，多细胞因子阵列， 高密度脂蛋白抗氧化活性测定），并将监督与阿尔茨海默氏症风险相关的新分析物测试 与辅助U 01应用程序相关的疾病和相关痴呆症（ADRD）。为了突出生物核心的 根据经验水平，在前几个时期已经测试了100 000多个样本。血液处理和/或 Coe博士（PI，BioCore）实验室也经常使用细胞进行基因表达。科博士有三十多年的 具有内分泌和免疫检测的经验，包括多种体液、不同的检测平台 和细胞分离方案。总而言之，核心B提供了基础架构和所需资源，以确保 质量和可靠的生物标志物评估。