Core B -- BioCore

核心B——BioCore

• 批准号: 10707318
• 负责人: CHRISTOPHER L COE
• 金额: $ 62.68万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-25 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707318
• 关键词: Aliquot; Alzheimer' s disease related dementia; Alzheimer' s disease risk; Antioxidants; Biological; Biological Assay; Biological Markers; Blood; Blood Tests; Body Fluids; Catecholamines; Cell Separation; Cells; Certification; Clinical; Code; Collection; Consultations; Data; Data Files; Development; Dryness; Education; Educational workshop; Endocrine; Ensure; Fluorescence; Freezing; Future; Gene Expression; Genetic Transcription; Health; Hematology; High Density Lipoproteins; Home; Hormones; Hydrocortisone; Immunoassay; Immunology procedure; Inflammatory; Information Dissemination; Infrastructure; Institutional Review Boards; Laboratories; Link; Liquid substance; Location; Mass Spectrum Analysis; Measures; Mentors; Metabolic; Methodology; Methods; Mission; Monitor; Needs Assessment; Nitrogen; Paper; Participant; Pathway interactions; Performance; Peroxidases; Preparation; Process; Protocols documentation; Public Domains; Publications; Quality Control; Records; Reporting; Research Personnel; Resources; Risk; Role; Saliva; Salivary; Sampling; Site; Specimen; Spottings; Standardization; System; Test Result; Testing; Time; Workload; biomarker panel; bone turnover; chemokine; cytokine; data sharing; diaries; experience; liquid chromatography mass spectrometry; meetings; operation; psychosocial; quality assurance; repository; transmission process; urinary

ABSTRACT – BIOCORE To obtain the full panel of biomarkers in the MIDUS project, collection and analytic efforts must be coordinated. The BioCore (B) thus fulfills an essential role in MIDUS by providing centralized, reliable testing, processing, and storage of biospecimens (i.e., hematological, endocrine, inflammatory, metabolic, bone turnover, antioxidant, gene expression). All tests are performed by single laboratories to ensure consistent results over time. Core B also assists in the standardization of protocols (i.e., identical collection and processing SOPs) and monitors shipment integrity and testing at the analytic laboratories over time (i.e., Quality Control). Core B also has the primary responsibility for storing aliquots from new samples and maintaining a complete set of archival specimens in ultracold freezers. In addition to overseeing all testing, the BioCore conducts assays and transports the samples that are tested at local clinical labs in Madison, WI. A centralized Core operation is essential to ensure uniform entry of test results into a single record system. One corollary of this administrative function is reviewing the reliability of data entry before transmission of results for dissemination and combining with demographic/psychosocial data. Core B also has several advisory and pedagogical functions, which includes providing “norms” for test results, meeting with investigators to advise on analyses, and conducting educational workshops on the use and meaning of biological measures at MIDUS meetings. The future performance of the BioCore will be facilitated by the extensive experience gained during the prior MIDUS periods. The panel of biomarkers going forward is similar to the panels employed previously with some new additions. The BioCore has previously assisted in establishing new methodologies (e.g., multi-cytokine arrays, an assay of HDL anti-oxidant activity) and will also oversee new analyte testing related to risk for Alzheimer’s Disease and Related Dementias (ADRD) linked with the Ancillary U01 application. To highlight the BioCore’s level of experience, over 100,000 specimens have been tested in the prior periods. Processing of blood and/or cells for gene expression is also routine for Dr. Coe’s (PI, BioCore) laboratory. Dr. Coe has over 3 decades of prior experience with endocrine and immune assays, including with many body fluids, different assay platforms and cell separation protocols. In sum, Core B provides the infrastructure and required resources to ensure high quality and reliable biomarker assessments in MIDUS.

摘要--生物核心 为了在MIDUS项目中获得完整的生物标志物小组，必须协调收集和分析工作。 因此，BioCore(B)通过提供集中、可靠的测试、处理 和储存生物标本(即血液、内分泌、炎症、代谢、骨转换、 抗氧化剂、基因表达)。所有测试都由单个实验室执行，以确保结果的一致性 时间到了。核心B还协助协议的标准化(即，相同的收集和处理SOP)和 在一段时间内监控分析实验室的装运完整性和测试(即质量控制)。核心B也 主要负责存储新样品的等分数据，并维护完整的档案 标本放在超低温冷冻室里。除了监督所有测试外，BioCore还进行检测和 运送在威斯康星州麦迪逊当地临床实验室进行测试的样本。集中的核心操作是 这对于确保将测试结果统一输入单一记录系统至关重要。这一行政管理的一个推论 功能是在传输结果以供分发和合并之前审查数据输入的可靠性 使用人口统计学/心理社会数据。核心B还具有若干咨询和教学职能，其中 包括为检测结果提供“标准”，与调查人员会面，就分析提供建议，以及进行 在MIDUS会议上举办关于生物措施的使用和意义的教育讲习班。未来 BioCore的性能将得益于在之前的MIDUS期间获得的广泛经验 句号。未来的生物标志物小组与以前使用的小组类似，新的 加法。BioCore以前曾协助建立新的方法(例如，多细胞因子阵列， 高密度脂蛋白抗氧化活性的测试)，并将监督与阿尔茨海默氏症风险相关的新分析物测试 与辅助U01应用程序相关的疾病和相关痴呆(ADRD)。为了突出BioCore的 根据经验水平，在前几个时期已经测试了100,000多个样本。血液和/或加工 基因表达细胞也是科博士实验室的常规工作。科博士已经有30多年的 有内分泌和免疫检测经验，包括使用多种体液、不同的检测平台 和细胞分离协议。总而言之，核心B提供基础设施和所需的资源，以确保高 MIDUS的质量和可靠的生物标记物评估。