Core B -- BioCore

核心B——BioCore

• 批准号: 10707318
• 负责人: CHRISTOPHER L COE
• 金额: $ 62.68万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-25 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707318
• 关键词: Aliquot; Alzheimer' s disease related dementia; Alzheimer' s disease risk; Antioxidants; Biological; Biological Assay; Biological Markers; Blood; Blood Tests; Body Fluids; Catecholamines; Cell Separation; Cells; Certification; Clinical; Code; Collection; Consultations; Data; Data Files; Development; Dryness; Education; Educational workshop; Endocrine; Ensure; Fluorescence; Freezing; Future; Gene Expression; Genetic Transcription; Health; Hematology; High Density Lipoproteins; Home; Hormones; Hydrocortisone; Immunoassay; Immunology procedure; Inflammatory; Information Dissemination; Infrastructure; Institutional Review Boards; Laboratories; Link; Liquid substance; Location; Mass Spectrum Analysis; Measures; Mentors; Metabolic; Methodology; Methods; Mission; Monitor; Needs Assessment; Nitrogen; Paper; Participant; Pathway interactions; Performance; Peroxidases; Preparation; Process; Protocols documentation; Public Domains; Publications; Quality Control; Records; Reporting; Research Personnel; Resources; Risk; Role; Saliva; Salivary; Sampling; Site; Specimen; Spottings; Standardization; System; Test Result; Testing; Time; Workload; biomarker panel; bone turnover; chemokine; cytokine; data sharing; diaries; experience; liquid chromatography mass spectrometry; meetings; operation; psychosocial; quality assurance; repository; transmission process; urinary

ABSTRACT – BIOCORE To obtain the full panel of biomarkers in the MIDUS project, collection and analytic efforts must be coordinated. The BioCore (B) thus fulfills an essential role in MIDUS by providing centralized, reliable testing, processing, and storage of biospecimens (i.e., hematological, endocrine, inflammatory, metabolic, bone turnover, antioxidant, gene expression). All tests are performed by single laboratories to ensure consistent results over time. Core B also assists in the standardization of protocols (i.e., identical collection and processing SOPs) and monitors shipment integrity and testing at the analytic laboratories over time (i.e., Quality Control). Core B also has the primary responsibility for storing aliquots from new samples and maintaining a complete set of archival specimens in ultracold freezers. In addition to overseeing all testing, the BioCore conducts assays and transports the samples that are tested at local clinical labs in Madison, WI. A centralized Core operation is essential to ensure uniform entry of test results into a single record system. One corollary of this administrative function is reviewing the reliability of data entry before transmission of results for dissemination and combining with demographic/psychosocial data. Core B also has several advisory and pedagogical functions, which includes providing “norms” for test results, meeting with investigators to advise on analyses, and conducting educational workshops on the use and meaning of biological measures at MIDUS meetings. The future performance of the BioCore will be facilitated by the extensive experience gained during the prior MIDUS periods. The panel of biomarkers going forward is similar to the panels employed previously with some new additions. The BioCore has previously assisted in establishing new methodologies (e.g., multi-cytokine arrays, an assay of HDL anti-oxidant activity) and will also oversee new analyte testing related to risk for Alzheimer’s Disease and Related Dementias (ADRD) linked with the Ancillary U01 application. To highlight the BioCore’s level of experience, over 100,000 specimens have been tested in the prior periods. Processing of blood and/or cells for gene expression is also routine for Dr. Coe’s (PI, BioCore) laboratory. Dr. Coe has over 3 decades of prior experience with endocrine and immune assays, including with many body fluids, different assay platforms and cell separation protocols. In sum, Core B provides the infrastructure and required resources to ensure high quality and reliable biomarker assessments in MIDUS.

摘要 - 野生动物 为了获得MIDUS项目中的完整生物标志物，必须协调收集和分析工作。 因此，Biocore（B）通过提供集中的，可靠的测试，处理， 和生物测量的存储（即血液学，内分泌，炎症，代谢，骨转换， 抗氧化剂，基因表达）。所有测试均由单个实验室进行，以确保结果一致 时间。核心B还有助于协议的标准化（即相同的收集和处理SOP）和 随着时间的推移，在分析实验室（即质量控制）监视分析实验室的运输完整性和测试。核心也 负责从新样本中存储等分试样并维护一套完整的档案 超速冰柜中的标本。除了监督所有测试外，Biocore还进行测定和 运输在威斯康星州麦迪逊市的当地临床实验室测试的样品。集中式核心操作是 确保将测试结果统一进入单个记录系统至关重要。此行政的一个推论 功能是在传播结果之前审查数据输入的可靠性以进行传播和组合 使用人口/社会心理数据。核心B还具有多种咨询和教学功能， 包括提供“规范”以进行测试结果，与调查人员会面以建议分析并进行进行。 关于中US会议的生物学措施的使用和意义的教育研讨会。未来 在上一届MIDUS期间获得的丰富经验将准备生物园的性能 时期。前来的生物标志物面板类似于以前使用一些新的面板 加法。 Biocore先前已协助建立新方法（例如，多环阵阵列， HDL抗氧化活性的测定），还将监督与阿尔茨海默氏症风险有关的新分析物测试 与辅助U01应用相关的疾病和相关痴呆症（ADRD）。突出野生 经验水平，在前期间已经测试了超过100,000个标本。血液的处理和/或 COE博士（PI，Biocore）实验室的基因表达细胞也是常规的。 Coe博士拥有超过3年的 先前具有内分泌和免疫测定的经验，包括许多体液，不同的测定平台 和细胞分离方案。总而言之，核心B提供基础架构和所需资源以确保高 MIDUS的质量和可靠的生物标志物评估。