Cell Isolation and Organ Function Core

细胞分离和器官功能核心

• 批准号: 10630737
• 负责人: Hongwei Yao
• 金额: $ 26.97万
• 依托单位: OCEAN STATE RESEARCH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630737
• 关键词: Acute Respiratory Distress Syndrome; Arterial Fatty Streak; Asthma; Atherosclerosis; Biology; Blood Vessels; Bronchopulmonary Dysplasia; COVID-19/ARDS; Cardiac; Cardiac Myocytes; Cardiopulmonary; Cell Separation; Cell physiology; Cells; Centers of Research Excellence; Collaborations; Communities; Congenital Heart Defects; Consumption; Coronary; Coronary Arteriosclerosis; Data Analyses; Development; Disease; Endothelial Cells; Endothelium; Experimental Designs; Fibroblasts; Fostering; Functional disorder; Generations; Goals; Heart; Heart Diseases; Hypoxemia; Individual; Inflammation; Injury; Interruption; Ischemia; Lung; Lung Diseases; Measurement; Mutation; Myocardial Reperfusion Injury; Natural regeneration; Obstruction; Organ; Pathogenesis; Perfusion; Phase; Pilot Projects; Prevention; Productivity; Pulmonary Hypertension; Reperfusion Injury; Reproducibility; Research; Research Personnel; Rhode Island; Services; Standardization; Systemic hypertension; Technical Expertise; Techniques; Technology; Thrombosis; Tissues; Training; Translating; Vascular Diseases; Vascular remodeling; Vascular resistance; Vascularization; Vasomotor; Work; cost; data acquisition; idiopathic pulmonary fibrosis; injury and repair; insight; instrument; lung vascular injury; pandemic disease; programs; quality assurance; repaired; response to injury; restoration; skills; temporal measurement; tool; transcription factor; vascular injury; ventilation; vessel regression

Cell Isolation and Organ Function (CIOF) Core The central theme of the CardioPulmonary Vascular Biology (CPVB) COBRE is to facilitate high impact vascular biology research and enable investigators to develop insights into basic mechanisms of vasculopathies and translate these findings into prevention or treatment for heart, lung, and vessel diseases. An increased understanding of the mechanisms regulating vascularization and repair may lead to unique approaches to interrupt disease pathogenesis or to promote or enhance cardiopulmonary vascular and vessel restoration. The Cell Isolation and Organ Function (CIOF) core provides a unique skill set and expertise to Rhode Island vascular biologists by providing quality assurance in isolation, characterization, and propagation of vascular derived cells and fibroblasts and cardiopulmonary ex vivo organ function analyses. The centralization of the cell and ex vivo organ isolation, characterization, and function measurements has helped by standardizing technique and reproducibility, and in turn provided investigators the technical expertise, thus, minimizing variability and providing uniformity in data acquisition for CPVB investigators. The services provided by the CIOF Core have permitted the Investigators to focus their efforts on aspects of their research endeavors related to experimental design, execution and interpretation. During Phase I and II, the CIOF core assisted 78 unique users, provided 5366 services, and performed ~$53,000 worth of services in year 9. Further, CIOF provided training for 36 individuals and provided data analyses for 370 requests (despite pandemic shutdown). The specific aims of the CIOF core are to: i) offer effective and reproducible services in cell isolation and ex vivo organ function; ii) acquire, establish, and disseminate technologies and instrument capabilities to provide state of the art research tools for the scientific community; iii) work with IDeA programs in Rhode Island (RI) and other states to foster scientific networks and collaborations by providing technical services to enhance research productivity.; and iv) maintain a robust line of services that meet the research needs of the scientific community. The CIOF core will continue to support and enhance vascular biology research, including for new pilot project investigators and the scientific community, in developing, facilitating, implementing, and performing technical services to impact the research productivity of our IDeA colleagues and collaborators.

细胞分离和器官功能（CIOF）核心 肺血管生物学（CPVB）COBRE的中心主题是促进高冲击性血管 生物学研究，使研究人员能够深入了解血管病变的基本机制， 将这些发现转化为心脏、肺和血管疾病的预防或治疗。增加 了解调节血管形成和修复的机制可能会导致独特的方法， 中断疾病发病机理或促进或增强心肺血管和血管恢复。的 细胞分离和器官功能（CIOF）核心为罗得岛血管提供了独特的技能和专业知识 生物学家通过在血管衍生细胞的分离、表征和增殖方面提供质量保证， 以及成纤维细胞和心肺离体器官功能分析。细胞的集中化和离体 器官分离、表征和功能测量有助于标准化技术， 再现性，反过来又为研究者提供了技术专长，从而最大限度地减少了变异性， 为CPVB研究者提供数据采集的统一性。CIOF Core提供的服务包括： 允许研究人员将精力集中在与实验相关的研究工作方面， 设计、执行和解释。在第一和第二阶段，CIOF核心协助了78个独特的用户， 5366次服务，并在第9年提供了价值约53，000美元的服务。此外，CIOF还为36名 （尽管大流行病关闭，）为370项请求提供数据分析。该委员会的具体目标 CIOF的核心是：i）在细胞分离和离体器官功能方面提供有效和可重复的服务; ii） 获取、建立和传播技术和仪器能力，以提供最先进的研究 科学界的工具; iii）与罗得岛（RI）和其他州的IDEA计划合作， 通过提供技术服务来提高研究生产力，从而建立科学网络和进行合作。和iv） 保持一系列强有力的服务，以满足科学界的研究需求。CIOF核心将 继续支持和加强血管生物学研究，包括新的试点项目研究人员和 科学界在开发、促进、实施和提供技术服务以影响 我们的IDEA同事和合作者的研究生产力。