Cell Isolation and Organ Function Core

细胞分离和器官功能核心

• 批准号: 10630737
• 负责人: Hongwei Yao
• 金额: $ 26.97万
• 依托单位: OCEAN STATE RESEARCH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2028-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630737
• 关键词: Acute Respiratory Distress Syndrome; Arterial Fatty Streak; Asthma; Atherosclerosis; Biology; Blood Vessels; Bronchopulmonary Dysplasia; COVID-19/ARDS; Cardiac; Cardiac Myocytes; Cardiopulmonary; Cell Separation; Cell physiology; Cells; Centers of Research Excellence; Collaborations; Communities; Congenital Heart Defects; Consumption; Coronary; Coronary Arteriosclerosis; Data Analyses; Development; Disease; Endothelial Cells; Endothelium; Experimental Designs; Fibroblasts; Fostering; Functional disorder; Generations; Goals; Heart; Heart Diseases; Hypoxemia; Individual; Inflammation; Injury; Interruption; Ischemia; Lung; Lung Diseases; Measurement; Mutation; Myocardial Reperfusion Injury; Natural regeneration; Obstruction; Organ; Pathogenesis; Perfusion; Phase; Pilot Projects; Prevention; Productivity; Pulmonary Hypertension; Reperfusion Injury; Reproducibility; Research; Research Personnel; Rhode Island; Services; Standardization; Systemic hypertension; Technical Expertise; Techniques; Technology; Thrombosis; Tissues; Training; Translating; Vascular Diseases; Vascular remodeling; Vascular resistance; Vascularization; Vasomotor; Work; cost; data acquisition; idiopathic pulmonary fibrosis; injury and repair; insight; instrument; lung vascular injury; pandemic disease; programs; quality assurance; repaired; response to injury; restoration; skills; temporal measurement; tool; transcription factor; vascular injury; ventilation; vessel regression

Cell Isolation and Organ Function (CIOF) Core The central theme of the CardioPulmonary Vascular Biology (CPVB) COBRE is to facilitate high impact vascular biology research and enable investigators to develop insights into basic mechanisms of vasculopathies and translate these findings into prevention or treatment for heart, lung, and vessel diseases. An increased understanding of the mechanisms regulating vascularization and repair may lead to unique approaches to interrupt disease pathogenesis or to promote or enhance cardiopulmonary vascular and vessel restoration. The Cell Isolation and Organ Function (CIOF) core provides a unique skill set and expertise to Rhode Island vascular biologists by providing quality assurance in isolation, characterization, and propagation of vascular derived cells and fibroblasts and cardiopulmonary ex vivo organ function analyses. The centralization of the cell and ex vivo organ isolation, characterization, and function measurements has helped by standardizing technique and reproducibility, and in turn provided investigators the technical expertise, thus, minimizing variability and providing uniformity in data acquisition for CPVB investigators. The services provided by the CIOF Core have permitted the Investigators to focus their efforts on aspects of their research endeavors related to experimental design, execution and interpretation. During Phase I and II, the CIOF core assisted 78 unique users, provided 5366 services, and performed ~$53,000 worth of services in year 9. Further, CIOF provided training for 36 individuals and provided data analyses for 370 requests (despite pandemic shutdown). The specific aims of the CIOF core are to: i) offer effective and reproducible services in cell isolation and ex vivo organ function; ii) acquire, establish, and disseminate technologies and instrument capabilities to provide state of the art research tools for the scientific community; iii) work with IDeA programs in Rhode Island (RI) and other states to foster scientific networks and collaborations by providing technical services to enhance research productivity.; and iv) maintain a robust line of services that meet the research needs of the scientific community. The CIOF core will continue to support and enhance vascular biology research, including for new pilot project investigators and the scientific community, in developing, facilitating, implementing, and performing technical services to impact the research productivity of our IDeA colleagues and collaborators.

细胞分离和器官功能(CIOF)核心 心肺血管生物学(CPVB)会议的中心主题是促进高冲击性血管 生物学研究并使研究人员能够深入了解血管病变的基本机制和 将这些发现转化为心、肺和血管疾病的预防或治疗。增加了 对调节血管形成和修复的机制的理解可能会导致独特的方法 阻断疾病的发病机制或促进或加强心肺血管和血管的修复。这个 细胞分离和器官功能(CIOF)核心为罗德岛血管提供了独特的技能和专业知识 生物学家在分离、鉴定和繁殖血管衍生细胞方面提供质量保证 并对成纤维细胞和心肺体外器官功能进行分析。细胞和体外的集中化 器官分离、特征和功能测量通过标准化技术和 可重复性，进而为调查人员提供了技术专长，从而最大限度地减少了可变性和 为CPVB调查人员提供统一的数据采集。CIOF核心提供的服务有 允许研究人员将他们的努力集中在与实验相关的研究工作方面 设计、执行和解释。在第一和第二阶段，CIOF核心协助了78个独立用户，提供了 提供了5366项服务，在第9年提供了价值约53,000美元的服务。此外，CIOF还为36 并提供了370项请求的数据分析(尽管大流行已关闭)。《公约》的具体目标 CIOF的核心是：i)在细胞分离和体外器官功能方面提供有效和可重复的服务；ii) 获取、建立和传播技术和仪器能力，以提供最先进的研究 为科学界提供工具；iii)与罗德岛(RI)和其他州的IDEA项目合作，以促进 通过提供技术服务提高研究生产率，建立科学网络和开展合作。 保持一系列满足科学界研究需求的强有力的服务。CIOF核心将 继续支持和加强血管生物学研究，包括新的试点项目调查员和 科学界，在开发、促进、实施和执行技术服务方面，以影响 我们的创意同事和合作者的研究效率。