Leica Stellaris 8 Confocal for Imaging Core

Leica Stellaris 8 共焦成像核心

• 批准号: 10630453
• 负责人: Amy Beaven
• 金额: $ 60万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630453
• 关键词: 13 year old; 14 year old; Biological Sciences; Biology; Cell Communication; Cells; Cellular biology; Contract Services; Dedications; Developmental Biology; Ensure; Faculty; Funding; Future; Image; Immunology; Knowledge; Lasers; Light; Maryland; Microscope; Minor; Molecular; Molecular Biology; Neurobiology; Physiologic pulse; Preparation; RNA; Regulation; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Scanning; Services; Students; Synapses; System; Technology; Training; United States National Institutes of Health; Universities; Visual; base; college; confocal imaging; design; detector; equipment acquisition; experience; extracellular; fluorescence lifetime imaging; imaging capabilities; imaging software; imaging system; instrument; neuroregulation; next generation; pathogen; protein transport

The University of Maryland College Park (UMD) requests $600,000 to purchase a critically-needed Leica Stellaris 8 FALCON (FAst Lifetime CONtrast) laser scanning confocal microscope. A diverse base of 14 Life Science researchers (12 of whom are NIH-funded), who are all making significant and pioneering contributions to cross- disciplinary research at the interface between cell biology, molecular biology neurobiology, developmental biology, immunology, and host-pathogen interactions, will immediately benefit from the transformative imaging capabilities of the instrument. Need for the instrument is great, as it will replace a well-used 14-year-old Leica SP5x confocal that can no longer be upgraded and will lose guaranteed service support at the end of 2022. The IC currently houses two other confocal systems, a 13-year-old Zeiss LSM 710 and a Zeiss LSM 980 Airyscan 2, but the existing systems at UMD cannot accommodate all the imaging needs of the current SP5x users plus the anticipated future needs of the major and minor users on this proposal, many of whom are starting new imaging projects. The requested instrument is specifically designed to maintain the capabilities of the existing SP5x, plus provide new cutting-edge technologies needed to enhance the users’ NIH-funded research beyond what is currently possible via any other imaging system on campus. The Stellaris 8 will be equipped with a next- generation pulsed white light laser, multiple high-efficiency Power HyD detectors, and integrated fluorescence lifetime imaging (FLIM) software and support. The Stellaris 8 will be housed and maintained in the well- established Imaging Core (IC), which is managed by an experienced facility director and is accessible to all UMD faculty, students, and their collaborators. The IC makes significant contributions to NIH-supported research at UMD by providing extensive imaging services ranging from advice on sample preparation to comprehensive instrument training and assistance with image acquisition. The requested scanning confocal microscope will advance current knowledge on a broad range of research projects, including host-pathogen interactions, the molecular composition of synaptic connections in mammalian visual circuits, regulation of neuromodulatory transmitters, protein trafficking, cell-cell communication, and the biology of extracellular RNAs. UMD has dedicated significant matching funds toward the purchase of the instrument and operational support. Furthermore, support at the departmental level will ensure that the instrument is covered under a service contract for the lifetime of the instrument.

马里兰州大学公园（UMD）要求$ 600,000购买急需的Leica Stellaris 8猎鹰（快速终生对比度）激光扫描共聚焦显微镜。 14个生命科学的多元化基础 研究人员（其中12名是NIH资助），他们都为跨界做出了重要的贡献 细胞生物学，分子生物学神经生物学，发育学的界面纪律研究 生物学，免疫学和宿主 - 病原体相互作用将立即受益于变革性成像 乐器的功能。对乐器的需求很棒，因为它将取代使用良好的14岁的Leica SP5X公共焦点无法再升级，并在2022年底将失去保证的服务支持。 IC目前包含另外两个共聚焦系统，一个13岁的Zeiss LSM 710和一个Zeiss LSM 980 Airyscan 2， 但是UMD的现有系统无法满足当前SP5X用户的所有成像需求以及 这项建议的主要用户和未成年人的未来需求，其中许多人正在开始新的成像 项目。该请求的仪器专门设计用于维护现有SP5X的功能，此外 提供新的尖端技术，以增强用户的NIH资助的研究 目前可以通过校园的任何其他成像系统。 Stellaris 8将配备下一步 一代脉冲白光激光器，多个高效功率HYD探测器和集成的荧光 终身成像（FLIM）软件和支持。 Stellaris 8将被安置在井中 已建立的成像核心（IC），由经验丰富的设施总监管理，所有UMD都可以访问 教师，学生及其合作者。该IC对NIH支持的研究做出了重大贡献 UMD通过提供广泛的成像服务，从样本准备的建议到全面 仪器培训和图像获取的帮助。请求的扫描共聚焦显微镜将 提出有关广泛研究项目的当前知识，包括宿主 - 病原体相互作用， 哺乳动物视觉回路中突触连接的分子组成，神经调节的调节 发射机，蛋白质运输，细胞 - 细胞通信和细胞外RNA的生物学。 UMD有 专门的匹配资金用于购买工具和运营支持。 此外，部门级别的支持将确保该工具被涵盖服务合同 在仪器的一生中。