Electrokinetic lithography: in situ microengineering of anisotropic 3D collagen matrices

动电光刻：各向异性 3D 胶原基质的原位微工程

• 批准号: 10630918
• 负责人: Vinay V Abhyankar
• 金额: $ 18.5万
• 依托单位: ROCHESTER INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630918
• 关键词: 3-Dimensional; Address; Affect; Architecture; Biocompatible Materials; Biomedical Research; Biomimetics; Biopolymers; Cell Communication; Cells; Charge; Collaborations; Collagen; Collagen Fiber; Collagen Type I; Communication; Cues; Deposition; Development; Developmental Biology; Disease; Endothelial Cells; Engineering; Environment; Epithelium; Extracellular Matrix; Fiber; Future; Gel; Goals; Heterogeneity; Immune; In Situ; Invaded; Laboratories; Letters; Malignant Neoplasms; Microfluidics; Mission; Morphogenesis; Motion; National Institute of General Medical Sciences; Outcome; Population; Predictive Value; Process; Program Development; Property; Proteins; Reporting; Research; Research Support; Resolution; Shapes; Speed; Stimulus; Surface; System; T-Lymphocyte; Techniques; Technology; Testing; Tissues; Traction; Work; Writing; biofabrication; bioprinting; cancer cell; cell motility; design; electric field; fabrication; fluid flow; insight; lithography; magnetic field; mechanical signal; meter; particle; prototype; real-time images; response; self assembly; success; technology development; technology research and development; tool; transmission process; tumor microenvironment

Summary In this project we will develop a technology called electrokinetic lithography (EKL) that will fill a technology void in the current state-of-the-art 3D aligned collagen fiber microengineering techniques. To mimic the structurally heterogenous environment found in the native extracellular matrix (ECM), we will sequentially combine extensional fluid flows with electric field driven bead motion (electrokinetics) to “write” cellular-scale discontinuities between domains of aligned collagen fibers within biomimetic 3D collagen gel. Technology development will be carried out with the followings aims: 1) Establish flow-based collagen fiber alignment and characterize electrokinetic transport parameters, and 2) Develop a microfluidic platform to engineer discontinuities within aligned 3D gel environments and validate cell motility responses. The success of this project will establish a transferrable lab prototype and support unprecedented studies that explore how cells respond to local disruptions in the aligned fibrous microarchitecture. Our technique will support new lines of exploration related to motility, sensing, and cell-cell communication within structurally heterogeneous environments, and address research questions cannot be currently answered with state-of- the-art collagen alignment techniques. This project directly aligns with the NIGMS mission of developing tools that enable potentially transformative biomedical research.

总结 在这个项目中，我们将开发一种称为电动光刻（EKL）的技术， 在当前最先进的3D排列胶原纤维微工程技术中是无效的。模仿 在天然细胞外基质（ECM）中发现的结构异质性环境，我们将依次 联合收割机将拉伸流体流动与电场驱动的珠运动（电动力学）结合以“写入”细胞尺度 仿生3D胶原凝胶内对齐的胶原纤维区域之间的不连续性。技术 本研究的目的是：1）建立基于流动的胶原纤维排列， 表征动电传输参数，以及2）开发微流体平台以工程化 在对齐的3D凝胶环境中的不连续性和验证细胞运动性响应。 该项目的成功将建立一个可转移的实验室原型，并支持前所未有的研究， 探索细胞如何应对对齐的纤维微结构中的局部破坏。我们的技术将 支持与结构内的运动性、感知和细胞间通讯相关的新探索路线 异构环境，并解决研究问题，目前无法回答的状态， 最先进的胶原蛋白排列技术该项目与NIGMS开发工具的使命直接一致 这使得潜在的变革性生物医学研究成为可能。

项目成果

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment.

• DOI: 10.3791/64457
• 发表时间: 2022-09-07
• 期刊: Journal of visualized experiments : JoVE
• 作者: Ahmed A;Joshi IM;Goulet MR;Vidas JA;Byerley AM;Mansouri M;Day SW;Abhyankar VV
• 通讯作者: Abhyankar VV