High-parametric flow cytometry cell sorter for human immunology studies.

用于人类免疫学研究的高参数流式细胞仪细胞分选仪。

• 批准号: 10632888
• 负责人: Niroshana Anandasabapathy
• 金额: $ 81.69万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2024-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632888
• 关键词: Antigen Presentation; Automobile Driving; B-Lymphocytes; Basic Science; Books; Cell Separation; Cell Survival; Cells; Childhood; Clinical Research; Cloning; Communities; Dedications; Discipline; Flow Cytometry; Funding; HIV; Human; Immune; Immune response; Immunity; Immunologic Monitoring; Immunology; Individual; Infection; Investigation; Lasers; Leadership; Medical center; Minor; Necrosis; Organ; Pathogenesis; Patients; Peripheral; Population; Research; Research Personnel; Sampling; Scientist; Site; Stream; Structure of germinal center of lymph node; Surveys; T-Lymphocyte; Technology; Tissues; Translational Research; United States National Institutes of Health; Work; equipment acquisition; insight; instrument; instrumentation; medical schools; prospective; tool; tumor; vaccine response

PROJECT SUMMARY Flow cytometry is the most commonly used and critical tool for analyzing immune cell populations in tissues, enabling approximately 18-20 parameter separation of populations and maintaining cell viability. By passing cells through a stream in which laser excitation is applied, individual populations of cells can be separated into either 96 well plates as single cells, enabling cloning and other applications, or can be enriched as populations in 4-6 separate populations simultaneously. This is the only cell separation technology universally adaptable to multiple tissues including isolation of rare populations from difficult sites such as peripheral tissues, necrotic tumors, and organs. Currently, this cutting-edge technology is not readily available to the Weill Cornell Medical College (WCMC) and medical center research community. Our current flow cytometry core is at maximum capacity usage for basic science applications and is booked weeks to months in advance, prohibiting alignment to the needs of clinical research investigators and basic and translational research directly on patient samples. Based on a prospective survey of WCMC scientists, we have identified over 12 NIH-funded major users, as well as a large number of minor users from multiple departments who have requested this instrumentation to advance their work. The proposed applications are diverse, leveraging the various scientific strengths of a number of WCMC labs. Some examples include Dr. Ari Melnick's work on understanding human B cell germinal centers, Dr. Sallie Permar’s understanding of pediatric infections and human vaccine responses and correlates of protective immunity, Dr. Laura Santambrogio’s ongoing studies on the human MHC peptidome to understand immune antigen presentation, and Dr. Brad Jones’ investigations into HIV pathogenesis and T cell immune responses. The leadership of WCMC is strongly supportive of the acquisition of this instrument, and will provide significant additional funds beyond those requested in the S10 application. In addition, dedicated space has been allocated within the Belfer Research Building and Human Immune Monitoring Core, where the instrument will be located and managed. Overall, we hypothesize that the establishment of a Flow Cytometry cell sorter instrument on the WCMC campus will be pivotal in driving new scientific insights across a number of projects and disciplines.

项目总结 流式细胞术是分析组织中免疫细胞群最常用和最关键的工具， 能够实现大约18-20个参数的种群分离并保持细胞活力。通过传递单元格 通过应用激光激发的水流，单个细胞群体可以被分离成 96个孔板作为单个细胞，允许克隆和其他应用，或者可以作为种群在4-6个中丰富 同时分离的种群。这是唯一一种普遍适用于多个 组织包括将稀有种群与周围组织、坏死性肿瘤等困难部位分离，以及 器官。 目前，威尔康奈尔医学院(WCMC)并不容易获得这项尖端技术 医学中心研究社区。我们目前的流式细胞仪核心处于基本的最大容量利用率 科学应用程序，提前几周到几个月预订，禁止与临床需求保持一致 研究人员以及直接对患者样本进行的基础和转译研究。 基于对WCMC科学家的前瞻性调查，我们还确定了NIH资助的12个主要用户 作为来自多个部门的大量次要用户，他们要求将此工具 他们的作品。提议的应用是多样化的，利用了许多 WCMC实验室。其中一些例子包括阿里·梅尔尼克博士对人类B细胞生发中心的理解， 萨利·佩尔马博士对儿科感染和人类疫苗反应的理解及其与 保护性免疫，Laura Santambrogio博士正在进行的对人类MHC多肽的研究 免疫抗原呈递，以及Brad Jones博士对HIV发病机制和T细胞免疫的研究 回应。 WCMC的领导层强烈支持收购这一工具，并将提供重要的 在S10申请中所要求的以外的额外资金。此外，还分配了专用空间 在贝尔福研究大楼和人类免疫监测核心内，仪器将放置在那里 并得到了管理。总体而言，我们假设建立一个流式细胞术细胞分选仪器在 WCMC校园将在推动许多项目和学科的新科学见解方面发挥关键作用。