High-parametric flow cytometry cell sorter for human immunology studies.

用于人类免疫学研究的高参数流式细胞仪细胞分选仪。

• 批准号: 10632888
• 负责人: Niroshana Anandasabapathy
• 金额: $ 81.69万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-15 至 2024-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632888
• 关键词: Antigen Presentation; Automobile Driving; B-Lymphocytes; Basic Science; Books; Cell Separation; Cell Survival; Cells; Childhood; Clinical Research; Cloning; Communities; Dedications; Discipline; Flow Cytometry; Funding; HIV; Human; Immune; Immune response; Immunity; Immunologic Monitoring; Immunology; Individual; Infection; Investigation; Lasers; Leadership; Medical center; Minor; Necrosis; Organ; Pathogenesis; Patients; Peripheral; Population; Research; Research Personnel; Sampling; Scientist; Site; Stream; Structure of germinal center of lymph node; Surveys; T-Lymphocyte; Technology; Tissues; Translational Research; United States National Institutes of Health; Work; equipment acquisition; insight; instrument; instrumentation; medical schools; prospective; tool; tumor; vaccine response

PROJECT SUMMARY Flow cytometry is the most commonly used and critical tool for analyzing immune cell populations in tissues, enabling approximately 18-20 parameter separation of populations and maintaining cell viability. By passing cells through a stream in which laser excitation is applied, individual populations of cells can be separated into either 96 well plates as single cells, enabling cloning and other applications, or can be enriched as populations in 4-6 separate populations simultaneously. This is the only cell separation technology universally adaptable to multiple tissues including isolation of rare populations from difficult sites such as peripheral tissues, necrotic tumors, and organs. Currently, this cutting-edge technology is not readily available to the Weill Cornell Medical College (WCMC) and medical center research community. Our current flow cytometry core is at maximum capacity usage for basic science applications and is booked weeks to months in advance, prohibiting alignment to the needs of clinical research investigators and basic and translational research directly on patient samples. Based on a prospective survey of WCMC scientists, we have identified over 12 NIH-funded major users, as well as a large number of minor users from multiple departments who have requested this instrumentation to advance their work. The proposed applications are diverse, leveraging the various scientific strengths of a number of WCMC labs. Some examples include Dr. Ari Melnick's work on understanding human B cell germinal centers, Dr. Sallie Permar’s understanding of pediatric infections and human vaccine responses and correlates of protective immunity, Dr. Laura Santambrogio’s ongoing studies on the human MHC peptidome to understand immune antigen presentation, and Dr. Brad Jones’ investigations into HIV pathogenesis and T cell immune responses. The leadership of WCMC is strongly supportive of the acquisition of this instrument, and will provide significant additional funds beyond those requested in the S10 application. In addition, dedicated space has been allocated within the Belfer Research Building and Human Immune Monitoring Core, where the instrument will be located and managed. Overall, we hypothesize that the establishment of a Flow Cytometry cell sorter instrument on the WCMC campus will be pivotal in driving new scientific insights across a number of projects and disciplines.

项目摘要 流式细胞仪是最常用和最关键的工具，用于分析组织中的免疫群群体， 实现大约18-20个种群的参数分离并维持细胞活力。通过通过细胞 通过应用激光兴奋的流，可以将单个细胞种群分为 96个井板作为单个单元，启用克隆和其他应用，或者可以在4-6中富集为种群 单独的人群。这是唯一普遍适用于多个的细胞分离技术 组织，包括从困难地点分离稀有种群的组织，例如外周组织，坏死肿瘤和 器官。 目前，Weill Cornell医学院（WCMC）和 医疗中心研究社区。我们当前的流式细胞仪核心的最大容量用途 科学应用程序并提前几个月预订，禁止对临床需求保持一致 研究人员以及基本和翻译的研究直接研究了患者样本。 根据对WCMC科学家的预期调查，我们还确定了超过12位由NIH资助的主要用户 作为来自多个部门的大量未成年人，他们要求此工具提前 他们的工作。拟议的应用是潜水员，利用了许多的各种科学优势 WCMC实验室。一些例子包括阿里·梅尔尼克（Ari Melnick）博士了解人类B细胞生发中心的工作， Sallie Permar博士对小儿感染和人类疫苗反应的理解以及 保护性免疫，Laura Santambrogio博士正在进行的关于人类MHC胡椒的研究 免疫抗原表现，布拉德·琼斯（Brad Jones）博士对HIV发病机理和T细胞免疫的研究 回答。 WCMC的领导能够强烈支持该工具的获取，并将提供重要的 额外的资金超出了S10应用程序中要求的资金。此外，专门的空间已分配 在Belfer研究大楼和人类免疫监测核心中，该仪器将位于该仪器 并管理。总体而言，我们假设建立流式细胞仪细胞分散器仪器 WCMC校园将在推动许多项目和学科的新科学见解方面至关重要。