Islet and Pancreas Analysis Core

胰岛和胰腺分析核心：项目摘要/摘要 胰岛和胰腺分析（IPA）核心成立于2007年，是由于对鼠胰岛素的需求不断增长。 来自范德比尔特糖尿病研究中心（VDRC）研究者的胰岛。在上一个供资周期，投资促进机构 核心为47名不同的调查人员（40名范德比尔特和7名外部非营利组织）提供了服务， 4,120例手术。VDRC的研究项目包括胰岛生物学， 发育以及在正常体内平衡和疾病中的功能，包括广泛的胰岛相关疾病 从细胞内信号传导，生理反应和适应，1型糖尿病的免疫学 糖尿病，胰岛富集转录因子的功能，以及正常胰岛分泌的维持 状态和细胞命运。在这些领域进行严格研究的关键是能够稳健地分离小鼠胰岛 并测定鼠和人胰岛的复杂生理功能。范德比尔特一直是 国家领导人在发展胰岛功能测定，证明了选择NIDDK支持的 综合胰岛分布计划（IIDP），以容纳人类胰岛表型分析计划（HIPP）， 范德比尔特在IPA核心总监Marcela Rissova的指导下。HIPP在范德比尔特的位置 使VDRC调查人员受益匪浅，使新开发的程序得以无缝实施 通过IPA核心。除了提供功能性检测外，IPA Core还提供VDRC研究人员 使用高分辨率的全载玻片图像扫描仪，可以同时分析两种组织结构， 和单细胞形态。许多VDRC调查人员利用成像基础设施和相关的 分析工具，并在这一竞争性的更新，IPA核心建议增加多重成像， 进行高影响力的组织研究。虽然获得这项技术的成本高昂， 对于单个实验室来说，这是一个挑战，将其作为核心服务提供将显著增强组织学的影响力。 小鼠和人体组织的分析。IPA核心还与动物代谢中心密切合作， 生理学核心（AMPC）标准化胰岛移植技术，一个宝贵的实验资源， 将补充现有的离体测定，并允许更多的研究人员进行体内研究。通过其 集中资源和专业知识，IPA核心为VDRC调查人员提供可靠和标准化的 促进胰岛生物学、细胞信号传导和代谢相关发现的程序。继续 IPA核心的卓越对于维持胰腺和胰岛领域的严格和有影响力的工作至关重要 VDRC研究人员的发育和生理学。

Islet and Pancreas Analysis Core: Project Summary/Abstract The Islet and Pancreas Analysis (IPA) Core was established in 2007 as a result of growing demand for murine islets from Vanderbilt Diabetes Research Center (VDRC) investigators. Over the last funding cycle, the IPA Core provided services to 47 different investigators (40 Vanderbilt and 7 external nonprofit) by assisting with 4,120 procedures. Research programs within the VDRC encompass the fields of pancreatic islet biology, development, and function in normal homeostasis and disease, including a broad spectrum of islet-related processes raging from intracellular signaling, physiological response and adaptation, the immunology of type 1 diabetes, the function of islet-enriched transcription factors, and the maintenance of normal hormone-secreting status and cell fate. Essential to rigorous research in these areas is the ability to robustly isolate murine islets and to assay the complex physiological function of both murine and human islets. Vanderbilt has been a national leader in developing islet functional assays, as evidenced by the selection of the NIDDK-supported Integrated Islet Distribution Program (IIDP) to house the Human Islet Phenotyping Program (HIPP) at Vanderbilt under direction of Marcela Brissova, IPA Core Director. The HIPP's location at Vanderbilt has greatly benefitted VDRC Investigators, allowing newly developed procedures to be seamlessly implemented through the IPA Core. In addition to offering functional assays, the IPA Core also provides VDRC investigators with high-resolution, whole-slide image scanners that enable simultaneous analysis of both tissue architecture and single cell morphology. Numerous VDRC investigators utilize the imaging infrastructure and associated analytical tools, and in this competitive renewal, the IPA Core proposes the addition of multiplex imaging to perform high impact, tissue-based studies. While access to this technology is cost-prohibitive and technically challenging for individual labs, offering it as a core service will significantly enhance the impact of histological analysis of mouse and human tissue. The IPA Core is also working closely with the Animal Metabolic Physiology Core (AMPC) to standardize islet transplantation techniques, a valuable experimental resource that will complement existing ex vivo assays and allow more investigators to perform in vivo studies. Through its centralized resources and expertise, the IPA Core provides VDRC investigators with reliable and standardized procedures that facilitate discoveries related to islet biology, cell signaling, and metabolism. The continued excellence of the IPA Core is crucial to sustain rigorous and impactful work in the areas of pancreas and islet development and physiology by VDRC investigators.