Islet Procurement and Analysis Core

胰岛获取和分析核心

• 批准号: 8469496
• 负责人: Marcela Brissova
• 金额: $ 9.68万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8469496
• 关键词: Area; Biological Assay; Biology; Development; Diabetes Mellitus; Equipment; Hormones; Human; Image Analysis; Immunology; Individual; Insulin-Dependent Diabetes Mellitus; Islets of Langerhans; Islets of Langerhans Transplantation; Knowledge; Measurement; Morphology; Mus; Postdoctoral Fellow; Price; Procedures; Process; Quality Control; RNA; Reporting; Research; Research Personnel; Scanning; Services; Signal Transduction; Slide; System; Techniques; Technology; Training; base; experience; graduate student; islet; morphometry; pancreatic islet function; tool; transcription factor

The Islet Procurement and Analysis Core (IPA Core) was expanded and became a new core of the Vanderbilt DRTC in June 2007. Soon after that the IPA Core was integrated into the Vanderbilt Core Ordering & Reporting Enterprise System. Islet biology, development, and function are major research areas of the Vanderbilt DRTC with investigators studying a variety of islet-related processes ranging from intracellular signaling, the immunology of type 1 diabetes, islet-enriched transcription factors, to islet transplantation. Necessary components of many of their experimental paradigms are high quality, well-characterized pancreatic islets, and reliable assays of islet function and mass. The main objective of the IPA Core is to provide DRTC-affiliated investigators with high quality mouse pancreatic islets. In addition, the core assists investigators in studies of islet function that involve services such as islet culture, islet perifusion, static islet incubation and RNA isolation. An important component of the IPA Core services is training. The IPA Core staff will continue to provide training to graduate students and postdoctoral fellows thus allowing them to gain knowledge and understanding of procedures used in their research. Due to high demand among DRTC-affiliated investigators, the IPA Core added to its equipment Aperio whole slide scanning system and will expand its service portfolio for the assessment of islet mass/morphology in this competitive renewal cycle. This will not only broaden the current DRTC user base, but will also allow us to reach out to non-VUMC users and offer slide scanning and image analysis services. Utilizing Aperio whole slide scanning technology and image analysis tools, the IPA Core will develop standards for pancreatic islet morphometry and measurement of islet mass. By having a centralized facility, the IPA Core provides services to a larger base of investigators at a lower price than would otherwise be possible. Other advantages are quality control, the ability to develop new techniques and applications, the increased potential for collaborative interactions, and immediate access to individuals with a broad experience and knowledge in handling isolated islets.

2007年6月，胰岛采购和分析核心（IPA核心）得到扩展，成为范德比尔特DRTC的新核心。不久之后，IPA Core被集成到范德比尔特Core订购和报告企业系统中。胰岛生物学、发育和功能是范德比尔特DRTC的主要研究领域，研究人员研究了各种胰岛相关过程，从细胞内信号传导、1型糖尿病的免疫学、胰岛富集转录因子到胰岛移植。他们的许多实验范例的必要组成部分是高质量的，充分表征的胰岛，以及胰岛功能和质量的可靠测定。IPA Core的主要目标是为DRTC附属研究人员提供高质量的小鼠胰岛。此外，核心协助研究人员在胰岛功能的研究，涉及服务，如胰岛培养，胰岛灌流，静态胰岛孵育和RNA分离。IPA核心服务的一个重要组成部分是培训。IPA核心工作人员将继续为研究生和博士后研究员提供培训，从而使他们能够获得知识和了解他们研究中使用的程序。由于DRTC附属研究者的高需求，IPA Core在其设备中增加了Aperio全载玻片扫描系统，并将在此竞争性更新周期中扩大其胰岛质量/形态评估的服务组合。这不仅将扩大目前DRTC的用户群，还将使我们能够接触到非VUMC用户，并提供幻灯片扫描和图像分析服务。利用Aperio全载玻片扫描技术和图像分析工具，IPA Core将开发胰岛形态测量和胰岛质量测量的标准。由于有一个集中的设施，投资促进机构核心以低于其他可能的价格向更多的调查人员提供服务。其他优势是质量控制，开发新技术和应用的能力，增加合作互动的潜力，以及立即接触在处理孤立胰岛方面具有广泛经验和知识的个人。