Microscopy Core

显微镜核心

• 批准号: 10633134
• 负责人: Amelia K Linnemann
• 金额: $ 17.12万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10633134
• 关键词: 20 year old; Anesthesia procedures; Animals; Basic Science; Biological; Biological Assay; Biosensor; Brain; Budgets; Cell physiology; Cells; Cellular biology; Charge; Collection; Contract Services; Data Storage and Retrieval; Development; Diabetes Mellitus; Fluorescence; Fostering; Functional disorder; Goals; Grant; Hour; Human; Image Analysis; In Vitro; Indiana; Individual; Infrastructure; Islets of Langerhans Transplantation; Kidney; Knowledge; Laboratories; Liver; Manufacturer; Metabolic Diseases; Methods; Microscope; Microscopy; Mission; Modeling; Monitor; Mus; Muscle; Neurons; Operative Surgical Procedures; Pancreas; Performance; Physiology; Proteins; Reagent; Research; Research Personnel; Savings; Scanning; Schedule; Scientist; Services; System; Techniques; Three-Dimensional Image; Time; Training; Training Support; Translational Research; Validation; Viral Vector; Work; biological systems; capsule; cost; design; digital; digital imaging; discount; experience; fluorescence lifetime imaging; imaging approach; imaging modality; interest; intravital microscopy; islet; light microscopy; medical schools; multi-photon; multiphoton microscopy; novel; protein function; research study; technique development; tissue resource; two-dimensional; vector

ABSTRACT (MICROSCOPY CORE) The overall goal of the Microscopy Core is to provide IDRC investigators with effective access to advanced methods of light microscopy, particularly the powerful new techniques of intravital microscopy developed during the previous grant period. The Microscopy Core utilizes the extensive facilities of the Indiana Center for Biological Microscopy (ICBM), the School of Medicine's 20 year-old core microscopy facility. The Core is equipped with three point-scanning confocal/multiphoton excitation microscopes, as well as a spinning-disk confocal microscope and multiple digital workstations supporting quantitative two- and three-dimensional image analysis. The Core is fully equipped to support intravital microscopy, providing investigators with space in a surgical suite with all necessary supplies for surgery, anesthesia, and animal monitoring. The Microscopy Core benefits not only from the extensive facilities of the ICBM, but also the reliable performance of systems that are fastidiously maintained by ICBM staff and continuously supported with manufacturers' service contracts. Specifically, the Core will apply and adapt methods of intravital microscopy that the ICBM has developed over the past 12 years to studies of the pathophysiology of diabetes and will develop and implement novel assays of protein and cell function based upon fluorescent protein biosensors, applying a combined approach of spectral and time-resolved fluorescence quantification. The Microscopy Core is Directed by Dr. K. Dunn, with Dr. R. Day serving as Associate Director. The Core leverages the expertise of a staff scientist and technician, and interfaces closely with the Islet & Physiology Core of the IDRC to ensure that tissue resources and surgical expertise are tightly integrated. The Aims of the Microscopy Core include: (1) Implementation of methods of quantitative intravital microscopy. (2) Continued development of intravital microscopy methods that will be applied to biological systems of interest to IDRC investigators, including approaches for studies of muscle and neurons in the brain. (3) Implementation of fluorescent protein biosensors for intravital and in vitro studies, including the optimization of biosensors for confocal and multiphoton microscopy, the design of viral vectors, validation of biosensor performance, optimized methods of image collection and quantitative analysis. (4) Training in the methods of microscopy and image analysis developed by the core for investigators whose needs exceed the capacity of the core. (5) Technical support for laboratories lacking microscopy expertise to utilize additional challenging techniques; e.g., fluorescent biosensor-based in vitro studies, fluorescence lifetime microscopy).

摘要(显微岩心) 显微镜核心的总体目标是为红十字国际研究中心的调查人员提供有效的访问高级 光学显微镜的方法，特别是发展出强大的活体显微镜新技术 在之前的授权期内。显微镜核心利用印第安纳中心的广泛设施 生物显微镜(ICBM)，医学院已有20年历史的核心显微镜设备。核心是 配备三个点扫描共焦/多光子激发显微镜以及一个旋转盘 支持定量二维和三维的共焦显微镜和多个数字工作站 图像分析。核心完全配备支持活体显微镜，为调查人员提供空间 在外科套间里，有手术、麻醉和动物监测所需的所有用品。《显微镜》 核心利益不仅来自洲际弹道导弹的广泛设施，而且还包括系统的可靠性能 由洲际弹道导弹公司的工作人员精心维护，并不断得到制造商服务的支持 合同。具体地说，核心将应用和调整ICBM拥有的活体显微镜方法 在过去的12年里发展到糖尿病的病理生理学研究，并将开发和实施 基于荧光蛋白生物传感器的蛋白质和细胞功能的新分析 光谱和时间分辨荧光定量方法。显微镜核心由K. 戴恩博士担任副主任。核心利用工作人员科学家的专业知识和 技术人员，并与IDRC的胰岛和生理核心密切联系，以确保组织资源 和外科专业知识紧密结合在一起。显微镜核心的目标包括： (1)定量活体显微镜方法的实施。 (2)活体显微镜方法的继续发展，这些方法将应用于生物系统 IDRC调查人员的兴趣，包括研究大脑肌肉和神经元的方法。 (3)用于体内和体外研究的荧光蛋白生物传感器的实施，包括 共聚焦和多光子显微镜生物传感器的优化，病毒载体的设计，验证 生物传感器性能、图像采集和定量分析的优化方法。 (4)核心为调查人员开发的显微镜和图像分析方法培训 他们的需求超出了核心的能力。 (5)为缺乏显微镜专业知识的实验室提供技术支持，以利用额外的挑战性 技术；例如，基于荧光生物传感器的体外研究、荧光寿命显微镜)。