Decoding the Multifactorial Etiology of Neural Network Dysfunction in Alzheimer's Disease

解读阿尔茨海默病神经网络功能障碍的多因素病因

• 批准号: 10670331
• 负责人: YADONG HUANG
• 金额: $ 465.72万
• 依托单位: J. DAVID GLADSTONE INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10670331
• 关键词: Address; Algorithms; Alzheimer like pathology; Alzheimer' s Disease; Alzheimer' s disease brain; Amino Acid Sequence; Amino Acid Substitution; Amyloid beta-Protein; Apolipoprotein E; Apolipoproteins; Autopsy; Behavior; Behavioral; Biochemical; Bioinformatics; Biological Assay; Brain; Cell Nucleus; Cells; Cognitive deficits; Communities; Complex; Data; Data Science Core; Data Set; Databases; Development; Disease; Disease Progression; Electrophysiology (science); Ensure; Etiology; Experimental Designs; Functional disorder; Goals; Histopathology; Human; Hyperactivity; Impaired cognition; Infection; Interneurons; Intervention; Lead; Link; Modeling; Molecular; Mus; Nature; Neuroglia; Neurons; Neurosciences; Pathogenesis; Pathologic; Pathway interactions; Patients; Phenotype; Protein Isoforms; Public Health; Research; Research Activity; Role; Statistical Models; Stress; System; Systems Biology; Therapeutic; Therapeutic Intervention; Transgenic Organisms; Validation; Virulence Factors; apolipoprotein E-4; behavior test; brain dysfunction; brain tissue; cell type; cohort; data harmonization; diverse data; drug development; epigenomics; epileptiform; experimental study; familial Alzheimer disease; human data; human tissue; improved; in vivo; innovation; insight; knock-down; mouse model; network dysfunction; neural network; neuromechanism; neuropathology; neurophysiology; novel; novel strategies; novel therapeutic intervention; overexpression; prevent; programs; research and development; tau Proteins; transcriptomics; working group

OVERALL – SUMMARY Alzheimer’s disease (AD) is a major unresolved public health problem. Efforts to prevent or stall this disease have failed, in good part because of inadequate understanding of its complex pathogenesis. Mounting evidence suggest that neural network dysfunction may underlie or promote AD-related cognitive deficits and contribute to disease progression. Yet, the causes and consequences of this dysfunction and the therapeutic potential of counteracting it remain sorely understudied. Therefore, the overarching goal of this program project is to decode the multifactorial etiology of AD-related neural network dysfunction and to leverage the novel mechanistic insights we will gain toward the development of better therapeutic strategies. Through collaborative interactions among four projects and two cores, our program will use systems neuroscience (neurophysiology and behavior) in combination with systems biology (single-cell transcriptomics and epigenomics), as well as neuropathology and improved mouse models, to determine how copathogenic interactions among apolipoprotein (apo) E4, amyloid-b (Ab), and tau cause neural network dysfunctions and cognitive decline in AD. An Administrative Core will coordinate all activities. Projects 1–3 will use novel mouse models of sporadic and familial AD to study interactions of different apoE isoforms with wildtype (WT) human tau (Project 1) or APP/Ab (Project 2), or among apoE4, Ab, and tau that is WT or bears disease-associated amino acid substitutions (Project 3). Project 4 will carry out single-nucleus transcriptomic and epigenomic analyses on postmortem brain tissues from deeply phenotyped human AD cases to gain novel insights into the multifactorial etiology of the human condition, validate leads from mouse studies, and encourage backtranslation into the models. An Integrative Data-Science Core will help us integrate results from all projects through innovative statistical modeling. This approach will reveal which aspects of human AD are most faithfully reproduced in the mouse models and help establish the causal drivers of cell-specific alterations in the human tissues, increasing the mechanistic resolving power of the latter studies. Therapeutic interventions in mouse models will determine whether reducing apoE4 expression in specific cell types can block copathogenic effects of apoE4 and tau on brain functions (Project 1), modulating the activity of specific interneurons can counteract copathogenic effects of apoE4 and APP/Ab (Projects 2 and 4), and knocking down tau can prevent and reverse brain dysfunction in models expressing all three pathogenic factors (Project 3). Through these highly cohesive efforts, our program will dissect the multifactorial interactions among AD-related pathogenic factors, define their relative contributions to the complex pathogenesis of brain dysfunctions, and help distinguish among neuropathological alterations that cause, result from, or are coincidental to neural network dysfunctions and cognitive decline. Sharing the diverse data sets we will generate and disseminating the novel integrative approaches we plan to develop for their analysis could enhance the progress of many other groups working in AD research and drug development or biomedicine in general.

Overall—概要