Animal Core (Core D)

摘要：动物核心(核心D) 对脑肿瘤生物学和治疗的研究最好在模拟脑肿瘤的原位动物模型中进行 肿瘤的自然环境。从这些活体研究中得出有意义的结论的能力是巨大的 当使用统一的、容易复制的动物模型时，效果会增强。该核心还支持 研究人员使用了转基因小鼠模型(特别是RCAS/NTV-a模型)。目的 动物的核心是提供动物建模服务，集中专业知识，从而实现一致性 和重复性，允许在实验、研究小组和项目之间进行准确的比较 这个脑癌孢子。在目前的资助期间，动物核心进行了546项实验，涉及 超过15,000只动物，供孢子调查人员使用。在接下来的5年资助期内，动物核心将继续 为所有脑癌孢子学研究者提供专业服务。为此，核心将： 1)使用患者来源的胶质瘤干细胞系(GSCs)为原位动物实验提供支持， 目前被认为是人脑胶质瘤体内模型的金标准。这一核心具有 开发了大约100个GSC，其中40多个已经完全分子表征； 2)使用患者来源的肿瘤组织，为侧翼和原位动物实验提供支持 NOD.Cg-PrkdcsordIl2rgtm1Wjl/SzJ(NSG)小鼠植入手术室； 3)使用具有免疫能力的基因工程小鼠模型为动物实验提供支持 (全球环境管理系统)，特别是RCAS/NTV-a系统； 4)为利用免疫活性检测溶瘤腺病毒的原位动物实验提供支持 允许病毒复制的仓鼠模型； 5)为使用传统专业胶质瘤细胞系进行原位动物实验提供支持 人(U87、U251、LN229)和小鼠(GL261)； 6)支持使用生物发光成像(BLI)或磁共振进行活体成像 磁共振成像(MRI)以评估治疗策略的疗效。 这些服务是任务关键型服务，其中一项或多项服务由该项目的所有项目使用。

SUMMARY: ANIMAL CORE (CORE D) Investigations into brain tumor biology and therapy are best carried out in orthotopic animal models that mimic the natural milieu of the tumor. The ability to reach meaningful conclusions from these in vivo studies is greatly enhanced when uniform, readily reproducible animal models are used. The core also provides support for investigators using genetically engineered mouse models (in particular the RCAS/Ntv-a model). The purpose of the Animal Core is to provide an animal modeling service that centralizes expertise thereby achieving uniformity and reproducibility that permits accurate comparisons between experiments, research groups, and projects in this Brain Cancer SPORE. In the current funding period, the Animal Core performed 546 experiments involving over 15,000 animals for SPORE investigators. In the next 5-year funding period, the Animal Core will continue to provide expert services to all investigators in Brain Cancer SPORE. To this end, the Core will: 1) Provide support for orthotopic animal experiments using patient-derived glioma stem cell lines (GSCs), which are currently considered the gold standard in vivo model of human gliomas. This Core has developed approximately 100 GSCs, over 40 of which have been fully molecularly characterized; 2) Provide support for flank and orthotopic animal experiments using patient-derived tumor explants from the operating room implanted into NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice; 3) Provide support for animal experiments using immunocompetent genetically engineered mouse models (GEMMs), particularly the RCAS/Ntv-a system; 4) Provide support for orthotopic animal experiments testing oncolytic adenoviruses using immunocompetent hamster models that are permissive to viral replication; 5) Provide support for orthotopic animal experiments using traditional profession glioma cell lines from humans (U87, U251, LN229) and mice (GL261); 6) Provide support for in vivo imaging either with bioluminescence imaging (BLI) or magnetic resonance imaging (MRI) to assess efficacy of therapeutic strategies. These services are mission critical and one or more of these services are used by all Projects in this SPORE.