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Animal Core (Core D)

动物核心（核心D）

基本信息

批准号：
10476404
负责人：
Betty Kim
金额：
$ 19.36万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10476404
关键词：
Animal Experiments Animal Model Animals Bioluminescence Brain Brain Neoplasms CD8B1 gene Cell Line Development Evaluation Firefly Luciferases Funding Genetically Engineered Mouse Glioblastoma Glioma Gold Hamsters Harvest Health Human Human Cell Line Human Resources Image Immune Immunocompetent Immunologics Implant Injections Intracranial Neoplasms Intraperitoneal Injections Investigation Label Luciferases Magnetic Resonance Imaging Malignant neoplasm of brain Mesenchymal Mission Modeling Molecular Monitor Mus Oncolytic viruses Operating Rooms Oral Patients Reproducibility Research Research Personnel SCID Mice Service delivery model Services System TP53 gene Techniques Testing Therapeutic Tissues Treatment Efficacy Tumor Biology Tumor Cell Line Tumor-Derived U251 Virus Virus Replication Xenograft procedure bioluminescence imaging career experience experimental study genetic manipulation glioma cell line human model implantation in vivo in vivo Model in vivo imaging mouse model novel novel therapeutics oncolytic adenovirus patient derived xenograft model programs stem cells stem-like cell treatment response tumor tumor growth

项目摘要

SUMMARY: ANIMAL CORE (CORE D) Investigations into brain tumor biology and therapy are best carried out in orthotopic animal models that mimic the natural milieu of the tumor. The ability to reach meaningful conclusions from these in vivo studies is greatly enhanced when uniform, readily reproducible animal models are used. The core also provides support for investigators using genetically engineered mouse models (in particular the RCAS/Ntv-a model). The purpose of the Animal Core is to provide an animal modeling service that centralizes expertise thereby achieving uniformity and reproducibility that permits accurate comparisons between experiments, research groups, and projects in this Brain Cancer SPORE. In the current funding period, the Animal Core performed 546 experiments involving over 15,000 animals for SPORE investigators. In the next 5-year funding period, the Animal Core will continue to provide expert services to all investigators in Brain Cancer SPORE. To this end, the Core will: 1) Provide support for orthotopic animal experiments using patient-derived glioma stem cell lines (GSCs), which are currently considered the gold standard in vivo model of human gliomas. This Core has developed approximately 100 GSCs, over 40 of which have been fully molecularly characterized; 2) Provide support for flank and orthotopic animal experiments using patient-derived tumor explants from the operating room implanted into NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice; 3) Provide support for animal experiments using immunocompetent genetically engineered mouse models (GEMMs), particularly the RCAS/Ntv-a system; 4) Provide support for orthotopic animal experiments testing oncolytic adenoviruses using immunocompetent hamster models that are permissive to viral replication; 5) Provide support for orthotopic animal experiments using traditional profession glioma cell lines from humans (U87, U251, LN229) and mice (GL261); 6) Provide support for in vivo imaging either with bioluminescence imaging (BLI) or magnetic resonance imaging (MRI) to assess efficacy of therapeutic strategies. These services are mission critical and one or more of these services are used by all Projects in this SPORE.

摘要：动物核心（核心 D）对脑肿瘤生物学和治疗的研究最好在模拟的原位动物模型中进行肿瘤的自然环境。从这些体内研究中得出有意义的结论的能力非常重要当使用统一的、易于重复的动物模型时，效果会增强。该核心还提供支持研究人员使用基因工程小鼠模型（特别是 RCAS/Ntv-a 模型）。目的 Animal Core 旨在提供动物建模服务，集中专业知识，从而实现一致性和再现性，允许在实验、研究小组和项目之间进行准确的比较这个脑癌孢子。在当前资助期间，Animal Core 进行了 546 项实验，涉及为 SPORE 研究人员提供超过 15,000 只动物。在接下来的5年资助期内，动物核心将继续为脑癌 SPORE 的所有研究人员提供专家服务。为此，核心将： 1) 为使用患者来源的胶质瘤干细胞系（GSC）的原位动物实验提供支持，目前被认为是人类神经胶质瘤体内模型的金标准。该核心有开发了大约 100 个 GSC，其中 40 多个已完全进行分子表征； 2) 使用来自患者的肿瘤外植体为侧腹和原位动物实验提供支持手术室植入 NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) 小鼠； 3）为使用免疫活性基因工程小鼠模型的动物实验提供支持（GEMM），特别是 RCAS/Ntv-a 系统； 4) 为使用免疫活性测试溶瘤腺病毒的原位动物实验提供支持允许病毒复制的仓鼠模型； 5) 为使用传统专业胶质瘤细胞系的原位动物实验提供支持人类（U87、U251、LN229）和小鼠（GL261）； 6) 通过生物发光成像 (BLI) 或磁共振为体内成像提供支持成像（MRI）来评估治疗策略的功效。这些服务是关键任务，并且该 SPORE 中的所有项目都使用其中一项或多项服务。