权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Animal Core (Core D)

动物核心（核心D）

基本信息

批准号：
10246331
负责人：
Betty Kim
金额：
$ 16.9万
依托单位：
UNIVERSITY OF TX MD ANDERSON CAN CTR
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10246331
关键词：
Animal Experiments Animal Model Animals Bioluminescence Brain Brain Neoplasms CD8B1 gene Cell Line Development Evaluation Firefly Luciferases Funding Genetically Engineered Mouse Glioblastoma Glioma Gold Hamsters Harvest Health Human Human Cell Line Human Resources Image Immune Immunocompetent Immunologics Implant Injections Intracranial Neoplasms Intraperitoneal Injections Investigation Label Luciferases Magnetic Resonance Imaging Malignant neoplasm of brain Mesenchymal Mission Modeling Molecular Monitor Mus Oncolytic viruses Operating Rooms Oral Patients Reproducibility Research Research Personnel SCID Mice Service delivery model Services System TP53 gene Techniques Testing Therapeutic Tissues Treatment Efficacy Tumor Biology Tumor Cell Line Tumor-Derived U251 Virus Virus Replication Xenograft procedure bioluminescence imaging career experience experimental study genetic manipulation glioma cell line human model implantation in vivo in vivo Model in vivo imaging mouse model novel novel therapeutics oncolytic adenovirus patient derived xenograft model programs response stem cells stem-like cell tumor tumor growth

项目摘要

SUMMARY: ANIMAL CORE (CORE D) Investigations into brain tumor biology and therapy are best carried out in orthotopic animal models that mimic the natural milieu of the tumor. The ability to reach meaningful conclusions from these in vivo studies is greatly enhanced when uniform, readily reproducible animal models are used. The core also provides support for investigators using genetically engineered mouse models (in particular the RCAS/Ntv-a model). The purpose of the Animal Core is to provide an animal modeling service that centralizes expertise thereby achieving uniformity and reproducibility that permits accurate comparisons between experiments, research groups, and projects in this Brain Cancer SPORE. In the current funding period, the Animal Core performed 546 experiments involving over 15,000 animals for SPORE investigators. In the next 5-year funding period, the Animal Core will continue to provide expert services to all investigators in Brain Cancer SPORE. To this end, the Core will: 1) Provide support for orthotopic animal experiments using patient-derived glioma stem cell lines (GSCs), which are currently considered the gold standard in vivo model of human gliomas. This Core has developed approximately 100 GSCs, over 40 of which have been fully molecularly characterized; 2) Provide support for flank and orthotopic animal experiments using patient-derived tumor explants from the operating room implanted into NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) mice; 3) Provide support for animal experiments using immunocompetent genetically engineered mouse models (GEMMs), particularly the RCAS/Ntv-a system; 4) Provide support for orthotopic animal experiments testing oncolytic adenoviruses using immunocompetent hamster models that are permissive to viral replication; 5) Provide support for orthotopic animal experiments using traditional profession glioma cell lines from humans (U87, U251, LN229) and mice (GL261); 6) Provide support for in vivo imaging either with bioluminescence imaging (BLI) or magnetic resonance imaging (MRI) to assess efficacy of therapeutic strategies. These services are mission critical and one or more of these services are used by all Projects in this SPORE.

总结：动物核心（核心d）