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Staphylococcus Biology in Ocular Infections

眼部感染中的葡萄球菌生物学

基本信息

批准号：
10672933
负责人：
Michelle C Callegan
金额：
$ 36.25万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10672933
关键词：
Acute Address Adherence Adhesions Agonist Animals Anti-Inflammatory Agents Antibiotic Resistance Antibiotics Area Attention Bacteria Bacterial Adhesins Bacterial Eye Infections Bacterial Infections Binding Biology Blindness Blood-Retinal Barrier Bypass Cell Culture Techniques Cell Line Cells Communicable Diseases Cornea Data Development Disease Endophthalmitis Equation Event Experimental Designs Eye Eye Infections Future Genes Genus staphylococcus Goals Growth Health Immune Immune response Immune system Immunity In Vitro Infection Infection prevention Inflammation Inflammatory Inflammatory Response Invaded Keratitis Knowledge Mediating Modeling Morbidity - disease rate Multi-Drug Resistance Organism Pain Pathogenesis Pathogenicity Pathway interactions Patient-Focused Outcomes Patients Positioning Attribute Prevention Process Production Publishing Regulation Research Resistance Retina Risk Speed Staphylococcus aureus Strategic Planning Surface Testing Therapeutic Time Tissues Toxin Virulence Virulence Factors Vision Visual corneal epithelium design improved in vivo ineffective therapies innate immune pathways innovation leukotoxin methicillin resistant Staphylococcus aureus mutant negative affect preservation prevent programs response retinal damage therapeutic development therapeutic target

项目摘要

PROJECT SUMMARY / ABSTRACT Bacterial eye infections cause a significant number of cases of blindness worldwide. Staphylococcus aureus causes a majority of these infections of the cornea (keratitis) and interior of the eye (endophthal- mitis). S. aureus produces several adhesins on its surface which aid in tissue attachment, surface components which trigger innate immune pathways and inflammation, and toxins which disrupt barriers and kill immune cells. The coordinated synthesis of these virulence factors aids the organism in host survival, and the host is usually negatively affected. In the eye, this can manifest as a painfully damaged cornea or irreversibly damaged retina, resulting in significant vision loss. To make matters worse, S. aureus is considered a Serious Threat on the CDC’s list of Antibiotic Resistance Threats in the US. MRSA and multidrug resistance are now common in ocular isolates, elevating the risk of ocular infections that are difficult to treat. We and others have investigated the pathogenic mechanisms underlying ocular bacterial infections. For S. aureus, animal infection models and ocular cell lines have been used to investigate the areas noted above. We have a very good idea of which toxins damage the cornea and retina and which innate pathways are involved in corneal and intraocular inflammation. This proposal is focused on the factors involved in the earliest events in S. aureus ocular infections, events that, to date, have drawn minimal attention. This new R01 proposal is based on the global hypothesis that coordinated regulation of S. aureus adhesins and toxins facilitate adherence to tissue, barrier breach, and escape from the acute immune response. The scientific premise is based on preliminary and published data demonstrating that: 1) adhesins on the S. aureus surface, when absent, reduce adhesion to corneal cells, 2) leukotoxins, when absent, reduce virulence in keratitis and endophthalmitis, and 3) S. aureus breach of the blood-retina barrier appears to be adhesin- and toxin-mediated. These areas are investigated in three separate but related aims focused on early events in S. aureus keratitis and endophthalmitis. We will use well-characterized S. aureus virulence factor- defective mutants and feasible in vitro and in vivo infection models in rigorous and straightforward experiments designed to define the S. aureus factors important in these events. For patients with eye infections, ineffective treatment often equates with vision loss. Our approaches to addressing these gaps in our field are innovative, translationally relevant, and will move the ocular infectious disease field forward by identifying the S. aureus factors responsible for adhesion to tissue and circumvention of the acute response, possibly uncovering targets that lead to more rational use or development of therapeutics for prevention and treatment of infection. These studies are a logical extension of our ocular infection research program, and we are well positioned to contribute new and important information that will improve options for preventing infection and preserving vision.

项目摘要/摘要细菌性眼部感染在全球范围内导致相当数量的失明病例。葡萄球菌金黄色葡萄球菌引起的角膜感染(角膜炎)和眼内感染(眼内感染)占大多数。 Mitis)。金黄色葡萄球菌在其表面产生几种粘附素，有助于组织附着、表面成分引发先天免疫途径和炎症，以及破坏屏障并杀死免疫力的毒素细胞。这些毒力因子的协调合成有助于有机体的生存，宿主是通常会受到负面影响。在眼睛中，这可能表现为痛苦的角膜损伤或不可逆转。视网膜受损，导致严重的视力丧失。更糟糕的是，金黄色葡萄球菌被认为是一种严重的在美国疾病控制与预防中心的抗生素耐药性威胁名单上。MRSA和多药耐药现在在眼部隔离中很常见，增加了难以治疗的眼部感染的风险。我们和其他人研究了眼部细菌感染的致病机制。为利用金黄色葡萄球菌、动物感染模型和眼细胞系对上述区域进行了研究。我们非常清楚哪些毒素会损害角膜和视网膜，哪些是先天途径与角膜和眼内炎症有关。这项建议的重点是金黄色葡萄球菌眼部感染的最早事件，到目前为止，引起关注的事件很少。这一新的R01提案基于全球假说，即金黄色葡萄球菌的协调调节粘附素和毒素有助于粘连组织、突破屏障和逃避急性免疫。回应。科学前提是基于初步的和已发表的数据表明：1)粘附素在金黄色葡萄球菌表面，当缺乏时，减少与角膜细胞的黏附；2)白细胞毒素，当缺乏时，减少角膜炎和眼内炎的毒性，以及3)金黄色葡萄球菌突破血-视网膜屏障似乎是粘附素和毒素介导的。这些领域在三个独立但相关的目标中进行调查，重点放在早期金黄色葡萄球菌角膜炎和眼内炎事件。我们将使用金黄色葡萄球菌的毒力因子- 严格和直接实验中的缺陷突变体和可行的体外和体内感染模型旨在定义在这些事件中重要的金黄色葡萄球菌因素。对于眼部感染的患者，无效的治疗通常等同于视力丧失。我们的方法是解决我们领域的这些差距是创新的，具有翻译相关性，并将推动眼部感染通过识别导致组织粘连和规避的金黄色葡萄球菌因子向前推进疾病领域可能会发现导致更合理地使用或发展预防和治疗感染的治疗学。这些研究是我们眼睛的合理延伸感染研究计划，我们处于有利地位，将贡献新的重要信息改进预防感染和保护视力的选择。