Genomics and Cell Biology Core

项目总结/摘要 基因组学和细胞生物学核心的总体目标将是促进尖端技术的应用。 从基因组学、生物信息学、细胞生物学和免疫学技术到营养和代谢研究。这 将通过鼓励多学科方法和合作来研究营养相关的 主题，并克服使用复杂和昂贵的技术的障碍，提供适当的 为NORCH调查人员提供咨询和培训。基因组学和细胞生物学的指导思想 核心是，尽管许多项目可以通过使用复杂的基因组和细胞生物学来推进， 技术、它们的费用和首次使用的困难是一个重大的障碍。核心是具体 旨在通过为NORC-H调查人员提供个性化咨询和培训来克服这一障碍 这在大多数NGS、基因组学、生物信息学、细胞生物学或免疫学核心设施中通常不可用。 随着核心主任Fred Ausubel博士（自2009年以来一直担任基因组学核心主任）退休， 自1994年以来，该中心赠款的最初成立），阿莱西奥法萨诺博士，著名的腹腔疾病 研究员和以前的联合核心主任，承担了主任的作用。联合副主任 （生物信息学和下一代测序）Ruslan Sadreyev博士和联合副主任（细胞 生物学）史海宁博士将继续负责核心的各个部分。有5个具体目标 核心：1）提供与基因组学、细胞生物学和免疫学研究设计相关的咨询服务; 2） 提供空间、技术人员和具体的培训和监督，以利用专门技术 以及用于细胞生物学、免疫学和基因组学研究的设备，包括单细胞转录 和表观基因组分析，协助开发高含量的高通量测定， 自动显微成像、荧光激活细胞分选和动物模型的开发; 3） 提供与下一代测序相关的各种基因组学相关服务; 4）提供生物信息学 分析各种类型的高通量数据，包括批量和单细胞RNA-Seq，ATAC-seq，ChIP- Seq、CUT&RUN、Hi-C、宏基因组学、蛋白质组学以及分子途径的计算分析， 网络和表型;以及5）在基因组学的基本概念方面进行丰富的教程和讲习班， 生物信息学，细胞生物学，营养学和GI免疫学相关技术，并协调夏季 为本科生和医科学生提供实习机会。这将通过以下方式实现： 核心主任和工作人员的专业知识，在先进的生物信息学分析仪器的帮助下， 最先进的下一代测序仪器，自动显微镜和成像基于高 内容筛选分析，用于进行免疫学，细胞生物学和形态学的各种设备- 相关分析。

PROJECT SUMMARY/ABSTRACT The overall goal of the Genomics & Cell Biology Core will be to facilitate the application of cutting-edge genomics, bioinformatics, cell biology, and immunology techniques to nutrition and metabolic research. This will be accomplished by encouraging multi-disciplinary approaches and collaborations to study nutrition-related topics, and by overcoming the barriers to using complex and expensive techniques by providing appropriate consultation and training to NORCH Investigators. The guiding philosophy of the Genomics & Cell Biology Core is that although many projects can be advanced by the use of sophisticated genomic and cell biology techniques, their expense and difficulty of first use represent a significant barrier. The Core is specifically designed to overcome this barrier by providing personalized consulting and training to NORC-H investigators that is not typically available in most NGS, genomics, bioinformatics, cell biology, or immunology core facilities. Following the retirement of Core Director Dr. Fred Ausubel (who has been the Director of Genomics Core since the original inception of the Center grant since 1994), Dr. Alessio Fasano, a renowned Celiac disease researcher and previously and Co-Core Director, has assumed the role of Director. The Co-Associate Director (Bioinformatics and Next Generation Sequencing) Dr. Ruslan Sadreyev, and Co-Associate Director (Cell Biology) Dr. Haining Shi will remain in charge of respective parts of the Core. There are 5 specific Aims of the Core: 1) provide consultative services related to genomics, cell biology, and immunology research design; 2) provide space, technical personnel, and specific training and supervision for utilizing specialized techniques and equipment used in cell biology, immunology, and genomics research, including single-cell transcriptional and epigenomic profiling, assistance in the development of high-content high-throughput assays using automated microscopic imaging, fluorescent activated cell sorting, and development of animal models; 3) provide a variety of genomics-related services related to next-generation sequencing; 4) provide bioinformatic analysis of various types of high-throughput data, including bulk and single-cell RNA-Seq, ATAC-seq, ChIP- Seq, CUT&RUN, Hi-C, metagenomics, proteomics, as well as computational analysis of molecular pathways, networks and phenotypes; and 5) conduct enrichment tutorials and workshops in basic concepts in genomic, bioinformatic, cell biology, and nutrition and GI immunology-related techniques, and coordinate summer internships for undergraduates and medical students. This will be accomplished through the significant expertise of the Core Directors and staff, with the help of advanced instrumentation for bioinformatics analysis, most up-to-date next generation sequencing instruments, automated microscopy and imaging based high content screening assays, a variety of equipment for carrying out immunological, cell biology, and morphology- related assays.