Organoid Shared Resource

类器官共享资源-摘要 器官共享资源的建立是为了向癌症中心成员提供访问 培养和进行三维类器官实验所需的试剂、设备和专业知识 模型类器官技术于2012年引入冷泉港实验室癌症中心 由大卫Tuveson，与理由，这些三维文化概括了许多方面， 原发性肿瘤生物学比标准的二维细胞系更忠实。18癌症中心 成员实验室（47%的成员）在他们的研究中使用人类和小鼠类器官， 用于40种癌症中心出版物或预印本。为了支持越来越多地使用类器官，新的共享 Resource于2020年推出，配备专用实验室空间和员工。类器官共享资源正在 在此申请中提出作为一个新的癌症中心共享资源，由学院院长卡米拉多斯桑托斯， 哲学博士、和经理丹尼斯·普兰克博士共享资源为成员提供集中访问 用于产生和使用类器官培养物、方法培训和标准化培养的方案 试剂以及用于在二维细胞上进行高通量化学筛选的设备 线或类器官培养物。癌症中心成员将受益于人类类器官的生物储存库 这些模型已经过验证并得到了很好的表征。共享资源将能够为癌症提供 由于批量采购培养试剂，中心成员节省了大量成本， 内部生产培养基添加剂的方案。类器官的使用已经促进了一些合作， 癌症中心项目符合癌症中心的战略重点，包括调查癌症健康 不同人群的差异，整合定量和湿台生物学家，并扩大翻译 research.

ORGANOID SHARED RESOURCE – ABSTRACT The Organoid Shared Resource was established to provide Cancer Center members with access to the reagents, equipment, and expertise needed to culture and perform experiments with three-dimensional organoid models. The organoid technology was introduced to the Cold Spring Harbor Laboratory Cancer Center in 2012 by David Tuveson, with the rationale that these three dimensional cultures recapitulate numerous aspects of primary tumor biology more faithfully than standard two-dimensional cell lines. There are now 18 Cancer Center member labs (47% of members) using human and murine organoids in their research, and organoids have been used in 40 Cancer Center publications or preprints. To support the growing use of organoids, the new Shared Resource was launched in 2020 with dedicated lab space and staff. The Organoid Shared Resource is being proposed in this application as a new Cancer Center Shared Resource, led by Faculty Head Camila dos Santos, Ph.D., and manager Dennis Plenker, Ph.D. The Shared Resource offers members centralized access to protocols for generating and working with organoid cultures, training in methods, and standardized culture reagents, as well as equipment for performing high-throughput chemical screens on either two dimensional cell lines or organoid cultures. Cancer Center members will benefit from access to a biorepository of human organoid models that have been validated and well characterized. The Shared Resource will be able to offer Cancer Center members substantial cost-savings due to bulk purchasing of culture reagents, and the development of protocols to produce media additives in-house. The use of organoids has already fostered several collaborative Cancer Center projects in line with Cancer Center strategic priorities, including investigating cancer health disparities in diverse populations, integrating quantitative and wet-bench biologists, and expanding translational research.