Experimental Mouse Shared Resource (EMSR)

实验小鼠共享资源：摘要 实验小鼠共享资源(EMSR)的目标是提高生产力和能力 亚利桑那大学癌症中心(UACC)成员通过提供体内模型的访问，包括 免疫受损和基因工程小鼠(GEM)模型。EMSR还提供了全面的 支持对啮齿动物(小鼠和大鼠)进行实验，并提供有效和高效的服务，使 UACC成员避免在没有这种共享的情况下发生的设备和培训重复 资源。重要的是，EMSR确保了技术干预的重复性，并提供了严格的 完成动物研究的信息量分析所需的质量控制。提供的主要服务 由EMSR组织的两个职能单位：(1)宝石生产单位，和(2)实验啮齿动物 单位。服务是完全互补的，共同产生有影响力的研究并帮助确保 为UACC成员提供资金。与宝石生成相关的其他服务包括鼠标转移 用于超低温保存的菌株，以及关于基因分型和育种策略的故障排除咨询。服务 可用于啮齿动物实验，包括接种各种异种移植和原位肿瘤模型 以及执行从常规注射到具有技术挑战性的外科手术等各种服务的专业知识 程序。EMSR执行从开发癌症遗传模型开始的所有方面的实验， 注射肿瘤细胞，治疗和测量用于药物测试的肿瘤，以及加工组织用于实验 终点，包括器官/组织冷冻保存的肉眼病理检查和准备 供UACC成员和其他共享资源检查的组织。在2020财年，EMSR为 101个唯一用户。在这些人中，56人(55%)是UACC成员，45人(45%)是非成员，其中包括8人 (8%)外部用户。UACC成员的使用包括来自癌症生物学计划的30名用户(54%)，19 临床和翻译肿瘤学的用户(34%)和癌症预防与控制中心的7(12%)用户 程序。EMSR为53份同行评审的出版物提供了捐助，并为222份赠款申请提供了支持 在这段时间。EMSR符合大学生物安全委员会的规定，啮齿类动物 实验是好的，实验室实习合格。

EXPERIMENTAL MOUSE SHARED RESOURCE: ABSTRACT The goal of the Experimental Mouse Shared Resource (EMSR) is to enhance the productivity and capabilities of University of Arizona Cancer Center (UACC) Members by providing access to in vivo models, including immunocompromised and genetically engineered mouse (GEM) models. EMSR also provides comprehensive support of experimentation in rodents (mouse and rat) and offers effective and efficient services that allow UACC Members to avoid duplication of equipment and training that would occur without such a Shared Resource. Importantly, EMSR ensures the reproducibility of technical interventions and provides the rigorous quality control that is required to complete informative analyses of animal studies. The primary services offered by EMSR are organized into two functional units: (1) a GEM Production Unit, and (2) the Experimental Rodent Unit. Services are completely complementary and together generate impactful research and help secure funding for UACC Members. Additional services associated with GEM generation include the transfer of mouse strains for cryopreservation and consulting on troubleshooting for genotyping and breeding strategies. Services offered for rodent experimentation include the inoculation of various xenograft and orthotopic tumor models and the expertise to perform services ranging from routine injections to technically challenging surgical procedures. EMSR performs all aspects of experimentation from developing genetic models of cancer, injecting tumor cells, treating and measuring tumors for drug testing, and processing tissues for experimental endpoints, including gross pathologic examinations with cryopreservation of organs/tissues and preparation of tissue for examination by UACC Members and other Shared Resources. In CY20, EMSR provided services to 101 unique users. Of these, 56 (55%) were UACC Members and 45 (45%) were non-Members, including eight (8%) external users. UACC Member usage included 30 users (54%) from the Cancer Biology Program, 19 users (34%) from Clinical & Translational Oncology, and 7 (12%) users from the Cancer Prevention & Control Program. EMSR contributed to 53 peer-reviewed publications and provided support to 222 grant applications over this period. EMSR is in compliance with the University’s Biosafety Committee rules and the Rodent Experimentation is good laboratory practice qualified.