Experimental Mouse Shared Resource (EMSR)

实验小鼠共享资源 实验小鼠共享资源（EMSR）的目标是提高生产力和能力 亚利桑那大学癌症中心（UACC）成员通过提供体内模型，包括 免疫受损和基因工程小鼠（GEM）模型。EMSR还提供全面的 支持啮齿动物（小鼠和大鼠）实验，并提供有效和高效的服务， UACC成员应避免设备和培训的重复，如果没有这样的共享， Resource.重要的是，EMSR确保了技术干预的可重复性，并提供了严格的 完成动物研究信息分析所需的质量控制。提供的主要服务 由EMSR组织成两个功能单元：（1）GEM生产单元，和（2）实验啮齿动物 单位服务是完全互补的，共同产生有影响力的研究，并帮助确保 为UACC成员提供资金。与GEM生成相关的其他服务包括鼠标转移 菌株的冷冻保存和基因分型和育种策略的故障排除咨询。服务 用于啮齿动物实验的疫苗包括接种各种异种移植物和原位肿瘤模型 和专业知识，以执行服务，从常规注射，以技术上具有挑战性的手术 程序. EMSR执行从开发癌症遗传模型， 注射肿瘤细胞，治疗和测量肿瘤用于药物测试，以及处理组织用于实验 终点，包括器官/组织冷冻保存的大体病理学检查和 供UACC成员和其他共享资源检查的组织。在CY 20，EMSR提供服务， 101个独立用户。其中，56人（55%）为UACC成员，45人（45%）为非成员，包括8名 （8%）外部用户。UACC会员使用包括来自癌症生物学项目的30名用户（54%）， 来自临床和转化肿瘤学的用户（34%）和来自癌症预防和控制的7名用户（12%） 程序. EMSR为53篇同行评审的出版物做出了贡献，并为222份赠款申请提供了支持 在此期间。EMSR符合大学生物安全委员会的规则和啮齿动物 实验符合药物非临床研究质量管理规范。

EXPERIMENTAL MOUSE SHARED RESOURCE: ABSTRACT The goal of the Experimental Mouse Shared Resource (EMSR) is to enhance the productivity and capabilities of University of Arizona Cancer Center (UACC) Members by providing access to in vivo models, including immunocompromised and genetically engineered mouse (GEM) models. EMSR also provides comprehensive support of experimentation in rodents (mouse and rat) and offers effective and efficient services that allow UACC Members to avoid duplication of equipment and training that would occur without such a Shared Resource. Importantly, EMSR ensures the reproducibility of technical interventions and provides the rigorous quality control that is required to complete informative analyses of animal studies. The primary services offered by EMSR are organized into two functional units: (1) a GEM Production Unit, and (2) the Experimental Rodent Unit. Services are completely complementary and together generate impactful research and help secure funding for UACC Members. Additional services associated with GEM generation include the transfer of mouse strains for cryopreservation and consulting on troubleshooting for genotyping and breeding strategies. Services offered for rodent experimentation include the inoculation of various xenograft and orthotopic tumor models and the expertise to perform services ranging from routine injections to technically challenging surgical procedures. EMSR performs all aspects of experimentation from developing genetic models of cancer, injecting tumor cells, treating and measuring tumors for drug testing, and processing tissues for experimental endpoints, including gross pathologic examinations with cryopreservation of organs/tissues and preparation of tissue for examination by UACC Members and other Shared Resources. In CY20, EMSR provided services to 101 unique users. Of these, 56 (55%) were UACC Members and 45 (45%) were non-Members, including eight (8%) external users. UACC Member usage included 30 users (54%) from the Cancer Biology Program, 19 users (34%) from Clinical & Translational Oncology, and 7 (12%) users from the Cancer Prevention & Control Program. EMSR contributed to 53 peer-reviewed publications and provided support to 222 grant applications over this period. EMSR is in compliance with the University’s Biosafety Committee rules and the Rodent Experimentation is good laboratory practice qualified.