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Acoustic platform for separation, isolation, and enrichment in biomedical research

用于生物医学研究中分离、隔离和富集的声学平台

基本信息

批准号：
10681223
负责人：
John Mark Meacham
金额：
$ 36.77万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2026-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10681223
关键词：
Acceleration Acoustics Address Affinity Antibodies Architecture Area Bacteria Biocompatible Materials Biological Biological Markers Biology Biomedical Research Biotechnology Cell Line Cell Separation Cell Size Cells Chemicals Computer Models Custom Detection Devices Diameter Dimensions Ensure Exposure to Fractionation Gene Expression Height Hela Cells Immunoassay Individual Investigation Label Lateral Legal patent Length Location MCF7 cell Magnetism Malignant Neoplasms Mammalian Cell Measurement Medicine Methods Microbe Microbial Biofilms Microbiology Microfluidic Microchips Microfluidics Mission Modeling Modification Monitor Motion National Institute of General Medical Sciences Operations Research Outcome Performance Play Porosity Process Property Proteomics Proxy Reaction Reagent Records Reproducibility of Results Research Research Methodology Role Sampling Series Shapes Site-Directed Mutagenesis Specific qualifier value Specificity Structure System Technology Time Translating Translations Validation Width antibody conjugate biological systems biomaterial compatibility cancer cell cell type chemical synthesis density design detection sensitivity experimental study fabrication flexibility high dimensionality imaging agent improved medical specialties meter microfluidic technology nanoscale new technology novel particle physical property prototype real time monitoring screening sound success technology development technology platform technology research and development tool transcriptomics ultrasound

项目摘要

PROJECT ABSTRACT This focused technology research and development project will deliver a new class of acoustic separation/en- richment tools for multiple biomedical research applications. Acoustic microfluidics has emerged as a key ena- bling technology in biology and medicine, providing unmatched capability for non-contact, label-free object ma- nipulation and analysis. The proposed microfluidic platform is based on a novel concept: a longitudinal standing bulk acoustic wave (LSBAW) subunit that controls micro- to nanoscale objects for functional separation and/or confinement. The patented LSBAW subunits are highly configurable, which allows arrays of repeated subunits to meet varying capacity and throughput needs, from monitoring/detection in small-volume (sub-µL) reaction chambers to high-throughput enrichment of rare species. Outcomes of this project will include purpose-built prototype systems for: (i) high-throughput enrichment/fractionation, (ii) process control at high capacity, and (iii) multiplexed analyses with real-time monitoring. To establish the versatility and utility of the LSBAW platform, different configurations will be validated in research applications of value to, for example, cancer biologists (rare cell enrichment), synthetic biochemists (antibody conjugate synthesis on ultrasound-confined reaction sub- strates), and microbiologists (monitoring/measurement of biological mechanisms in bacterial cells). The technol- ogy outcomes of this project will be relevant not only to those applications, but will be broadly applicable to any field that relies on separation, isolation, and enrichment. The project includes three Aims: Aim 1: Demonstrate scalability of LSBAW subunits for high-volume, high-throughput enrichment of rare species. Aim 2: Validate series configurations of LSBAW subunit arrays for high-capacity cell modification/labeling or custom biomolecule synthesis. Aim 3: Validate multiplexed configurations of LSBAW subunit arrays for quantification and/or detection of a target species or biological mechanism. Validation experiments will be used to rigorously assess capabilities that are relevant to specific applications. Use of standard models (e.g., microparticles as proxies for biological cells) or well-characterized biological sys- tems (e.g., commercial antibodies; standard mammalian cell lines, mixtures of cells, and microbes) will ensure consistency and reproducibility of results. In each application, success will be defined using quantitative perfor- mance criteria (e.g., throughput, capacity, specificity, sensitivity) and comparison with appropriate existing tools and methods. The team merges expertise in microfluidics, synthesis and characterization of imaging agents, microbiology, and rare cell isolation/analysis, with strong track records of technology development and deploy- ment. Completion of these aims will translate a novel acoustic microfluidics concept to a suite of powerful and broadly accessible research tools that will accelerate research in a multitude of biomedical research fields.

项目摘要