Experimental Mouse Resources Core

研究人员将利用血液学卓越合作中心 (CCEH) 进行体内研究 研究重点是参与稳态造血、骨 骨髓生态位、骨髓移植和免疫细胞功能。集中化将防止重复 资源，确保持续供应适应环境的小鼠，并维持高质量的研究基础设施 对于进行造血干细胞和祖细胞 (HSPC) 功能的体内研究至关重要。的 实验小鼠资源核心 (EMRC) 将为 CCEH 研究人员提供高级资源 使用小鼠进行小鼠和人类移植模型的研究。 EMRC 的具体目标是： 1. 维护重要小鼠的现场繁殖群体，以研究人类和小鼠的造血作用 体内。用于针对人类造血作用的研究，NOD.Cg-PrkdcscidIl2rgtm1Wjl/J (NSG) 集落 和 NOD.Cg-Ragtm1MomIl2rgtm1Wjl/SzJ 和 (NRG) 免疫缺陷小鼠将被保留。预计 未来的需求，表达人类 IL3 的三重转基因 NSG-SGM3 (NSGS) 小鼠的起始群体， GM-CSF和SCF已经建立并且可以根据需要进行扩展。繁殖群落 C57/BL/6J、B6.SJL-Ptprca Pepcb/BoyJ (Boy/J) 和 BoyJ x C57BL/6J (F1 杂交) 小鼠品系将 维持。 F1 杂交是一种独特的品系，无法在市场上买到。同类小鼠品系 允许同时跟踪供体、竞争者和受体细胞。 2. 为CCEH调查人员提供专业化的核心服务和咨询。 EMRC 人员将 进行放射、移植、外周血连续采样和复合给药。核心 将采集移植小鼠的组织并将其提供给研究人员，供 CCEH 进行详细分析 流式细胞仪和组织细胞仪核心。 ERMC 将与 Hypoxia Core 密切合作，协调 在缺氧与环境空气中收集和处理的小鼠和人类 HSPC 移植物 (Mantel 等人，细胞 2015）。 EMRC 主任将与核心人员协调所有程序、实验 设计、方法论和数据解释。专业知识和基础设施到位，以促进各个方面 为现场 CCEH 成员以及国家级 PI 提供无缝的 BMT 研究。 3. 作为CCEH实验室的教育资源。 EMRC 之间的卓越连接 其他生物医学核心以及浓缩核心将加强研究和培训 CCEH 会员及其学员的活动。高品质体内执行力培训 研究将是培训我们下一代科学家的关键和重要组成部分 在致力于非恶性血液学研究的研究事业中取得成功。 4. 与全国其他 CCEH 中心和调查人员合作，为以下人群提供 EMRC 服务： 参与非恶性血液学研究的科学家群体。

The Cooperative Centers of Excellence in Hematology (CCEH) will be utilized by investigators for in vivo studies focused on molecular and cellular mechanisms involved in steady state hematopoiesis, the bone marrow niche, BM transplantation, and immune cell function. Centralization will prevent duplication of resources, ensure consistent supply of acclimated mice, and maintain high quality research infrastructure essential for conducting in vivo studies of hematopoietic stem and progenitor cell (HSPC) function. The Experimental Mouse Resources Core (EMRC) will provide CCEH investigators advanced resources for their studies using mice for murine and human transplantation models. The specific aims of the EMRC are: 1. Maintain on-site breeding colonies of essential mice to study human and murine hematopoiesis in vivo. For investigations focused on human hematopoiesis, colonies of NOD.Cg-PrkdcscidIl2rgtm1Wjl/J (NSG) and NOD.Cg-Ragtm1MomIl2rgtm1Wjl/SzJ and (NRG) immunodeficient mice will be maintained. In anticipation of future needs, a starter colony of the triple transgenic NSG-SGM3 (NSGS) mice that express human IL3, GM-CSF and SCF has been established and can be expanded as needed. Breeding colonies of C57/BL/6J, B6.SJL-Ptprca Pepcb/BoyJ (Boy/J), and the BoyJ x C57BL/6J (F1 cross) mouse strains will be maintained. The F1 cross is a unique strain and is not commercially available. Congenic mouse strains allow for simultaneous tracking of donor, competitor, and recipient cells. 2. Provide specialized core services and consultation to CCEH investigators. EMRC personnel will conduct irradiations, transplantations, serial sampling of peripheral blood, and compound dosing. The Core will harvest and provide tissues from transplanted mice to investigators for detailed analysis by the CCEH Flow and Tissue Cytometry Core. The ERMC will work closely with the Hypoxia Core to coordinate transplant of mouse and human HSPC collected and processed under hypoxia versus ambient air (Mantel et al., Cell 2015). The EMRC Director will coordinate with Core personnel all procedures, experimental design, methodology, and data interpretation. Expertise and infrastructure is in place to facilitate all aspects of BMT studies seamlessly for on-site CCEH members as well as PIs on a national level. 3. Serve as an educational resource for CCEH laboratories. Exceptional connectivity between the EMRC and the other biomedical cores as well as the Enrichment core will augment the research and training activities of the CCEH membership and their trainees. Training in the execution of high-quality in vivo studies will be a critical and essential component in training our next generation of scientists to be successful in research careers dedicated to the study of nonmalignant hematology. 4. Work in concert with other CCEH centers and investigators Nationwide to provide EMRC services to the community of scientists involved in nonmalignant hematology research.