Experimental Mouse Resources Core

实验鼠标资源核心

• 批准号: 10681258
• 负责人: Karen Elizabeth Pollok
• 金额: $ 22.74万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10681258
• 关键词: AMD3100; Acclimatization; Adult; Air; Animals; Biology; Bone Marrow; Bone Marrow Transplantation; Breeding; CSF3 gene; Cell Culture Techniques; Cell physiology; Cells; Chimerism; Clinical; Collecting Cell; Communities; Congenic Mice; Consultations; Cytometry; Data Analyses; Dedications; Dose; Ensure; Experimental Designs; Faculty; Funding; Future; Goals; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Factor; Harvest; Hematology; Hematopoiesis; Hematopoietic; Hematopoietic stem cells; Human; Human Resources; Hypoxia; IL3 Gene; Immune; Immunodeficient Mouse; Individual; Infrastructure; Investigation; Laboratories; Medical Students; Methodology; Molecular; Mouse Strains; Mus; Non-Malignant; Procedures; Process; Production; Regulation; Research; Research Activity; Research Infrastructure; Research Personnel; Research Project Grants; Research Support; Resources; Sampling; Scientist; Services; Site; Technical Expertise; Therapeutic; Time; Tissue Transplantation; Tissues; Training; Training Activity; Transgenic Organisms; Transplantation; Umbilical Cord Blood; Work; boys; career; cell type; clinical efficacy; congenic; cost; design; education resources; experimental study; graduate student; in vivo; irradiation; member; next generation; peripheral blood; prevent; stem cell biology; stem cell function; transplant model

The Cooperative Centers of Excellence in Hematology (CCEH) will be utilized by investigators for in vivo studies focused on molecular and cellular mechanisms involved in steady state hematopoiesis, the bone marrow niche, BM transplantation, and immune cell function. Centralization will prevent duplication of resources, ensure consistent supply of acclimated mice, and maintain high quality research infrastructure essential for conducting in vivo studies of hematopoietic stem and progenitor cell (HSPC) function. The Experimental Mouse Resources Core (EMRC) will provide CCEH investigators advanced resources for their studies using mice for murine and human transplantation models. The specific aims of the EMRC are: 1. Maintain on-site breeding colonies of essential mice to study human and murine hematopoiesis in vivo. For investigations focused on human hematopoiesis, colonies of NOD.Cg-PrkdcscidIl2rgtm1Wjl/J (NSG) and NOD.Cg-Ragtm1MomIl2rgtm1Wjl/SzJ and (NRG) immunodeficient mice will be maintained. In anticipation of future needs, a starter colony of the triple transgenic NSG-SGM3 (NSGS) mice that express human IL3, GM-CSF and SCF has been established and can be expanded as needed. Breeding colonies of C57/BL/6J, B6.SJL-Ptprca Pepcb/BoyJ (Boy/J), and the BoyJ x C57BL/6J (F1 cross) mouse strains will be maintained. The F1 cross is a unique strain and is not commercially available. Congenic mouse strains allow for simultaneous tracking of donor, competitor, and recipient cells. 2. Provide specialized core services and consultation to CCEH investigators. EMRC personnel will conduct irradiations, transplantations, serial sampling of peripheral blood, and compound dosing. The Core will harvest and provide tissues from transplanted mice to investigators for detailed analysis by the CCEH Flow and Tissue Cytometry Core. The ERMC will work closely with the Hypoxia Core to coordinate transplant of mouse and human HSPC collected and processed under hypoxia versus ambient air (Mantel et al., Cell 2015). The EMRC Director will coordinate with Core personnel all procedures, experimental design, methodology, and data interpretation. Expertise and infrastructure is in place to facilitate all aspects of BMT studies seamlessly for on-site CCEH members as well as PIs on a national level. 3. Serve as an educational resource for CCEH laboratories. Exceptional connectivity between the EMRC and the other biomedical cores as well as the Enrichment core will augment the research and training activities of the CCEH membership and their trainees. Training in the execution of high-quality in vivo studies will be a critical and essential component in training our next generation of scientists to be successful in research careers dedicated to the study of nonmalignant hematology. 4. Work in concert with other CCEH centers and investigators Nationwide to provide EMRC services to the community of scientists involved in nonmalignant hematology research.

研究者将利用血液学卓越合作中心（CCEH）进行体内研究。 研究集中在稳态造血、骨生成、骨再生、骨 骨髓生态位、骨髓移植和免疫细胞功能。集中化将防止重复 资源，确保驯化小鼠的持续供应，并保持高质量的研究基础设施 这对于进行造血干细胞和祖细胞（HSPC）功能的体内研究至关重要。的 实验小鼠资源核心（EMRC）将为CCEH研究人员提供先进的资源， 使用小鼠作为鼠和人移植模型的研究。该公司的具体目标是： 1.维持基本小鼠的现场繁殖集落，以研究人类和小鼠的造血， vivo.对于关注人造血的研究，NOD. Cg-PrkdcscidIl 2 rgtm 1 Wjl/J（NSG）的菌落 和NOD. Cg-Ragtm 1 MomI 12 rgtm 1 Wjl/SzJ和（NRG）免疫缺陷小鼠。由于预期 未来需要，表达人IL 3的三重转基因NSG-SGM 3（NSGS）小鼠的起始集落， GM-CSF和SCF已经建立，并可根据需要扩大。繁殖群体 C57/BL/6 J、B6.SJL-Ptprca Pepcb/BoyJ（Boy/J）和BoyJ x C57 BL/6 J（F1杂交）小鼠品系将 树立政治意识F1杂交是一种独特的菌株，没有市售。同类系小鼠 允许同时追踪供体、竞争者和受体细胞。 2.为CCEH调查员提供专业的核心服务和咨询。机电工程管理局人员会 进行辐照、移植、外周血系列采样和化合物给药。核心 将采集移植小鼠的组织并提供给研究人员，供CCEH进行详细分析 流式细胞术和组织细胞术核心。ERMC将与低氧核心密切合作， 在低氧与环境空气下收集和处理的小鼠和人HSPC移植物（Mantel 例如，Cell 2015）。EMRC主任将与核心人员协调所有程序、实验 设计、方法和数据解释。专业知识和基础设施到位，以促进所有方面 为现场CCEH成员以及国家层面的PI提供无缝的BMT研究。 3.作为CCEH实验室的教育资源。与EMRC之间的卓越连接 其他生物医学核心以及浓缩核心将加强研究和培训 CCEH会员及其学员的活动。在执行高品质的培训在体内 研究将是培养我们下一代科学家的关键和必要组成部分， 在致力于非恶性血液学研究的研究事业中取得成功。 4.与全国其他CCEH中心和调查人员合作，为以下人员提供EMRC服务： 参与非恶性血液学研究的科学家团体。