Cytometry and Imaging Core

项目摘要：细胞学和成像核心 细胞测量和成像核心的主要目标是通过以下方式提高该计划的整体生产率 为激光扫描细胞术(LASC)、3D的使用提供经济高效的、集中的、必不可少的资源 成像、细胞预富集和流式细胞术。核心将提供i)LASC的集中资源，用于 所有3个计划项目；ii)项目1和2访问两个项目的集中式、成本效益高的流式细胞仪资源 流式细胞仪分析仪、16参数流式细胞仪Attune NXT和8参数流式细胞仪CANTO II；三) 为所有3个方案项目提供集中的3D共焦成像资源；iv)专家咨询资源和 在设计和分析复杂的多色流式细胞术和LASC实验方面提供咨询，包括 FlowJo软件许可用于流式细胞仪分析，ICYS细胞分析软件(CompuCyte)在 用于所有3个计划项目的LASC；以及v)集中的细胞浓缩资源，以节省时间和金钱 项目1和2。通过为使用LASC的研究项目提供具有成本效益的集中资源，3D 成像、细胞预富集和流式细胞术，细胞学和成像核心将有助于进一步 计划的目标是了解潜在的分子和细胞机制和 移植围手术期输血的有效性。

Project Summary: Cytometry and Imaging Core The key objective of the Cytometry and Imaging Core is to enhance the overall productivity of the Program by providing a cost-effective, centralized, essential resource for the use of laser scanning cytometry (LaSC), 3D imaging, cellular pre-enrichment, and flow cytometry. The Core will provide i) a centralized resource of LaSC for all 3 program projects; ii) a centralized, cost-effective resource of flow cytometry for Project 1 and 2 to access two flow cytometry analyzers, a 16-parameter flow cytometer Attune NxT and an 8-parameter flow cytometer Canto II; iii) a centralized resource of 3D confocal imaging for all 3 program projects; iv) a resource of expert advice and consultation in the design and analysis of complex, multicolor flow cytometry and LaSC experiments including FlowJo software licenses in flow cytometry analysis and iCys cytometric analysis software (CompuCyte) in LaSC for all 3 program projects; and v) a centralized resource of cell enrichment to save time and money for Project 1 and 2. By providing a cost-effective, centralized resource for research projects to use LaSC, 3D imaging, cellular pre-enrichment, and flow cytometry, the Cytometry and Imaging Core will help further the Programs goals of understanding the molecular and cellular mechanisms underlying the effect and effectiveness of blood transfusion in the peri-transplant setting.