Cytometry and Imaging Core

细胞计数和成像核心

• 批准号: 10682587
• 负责人: Shin-Young Park
• 金额: $ 32.75万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-15 至 2027-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10682587
• 关键词: 3-Dimensional; Antibodies; Apoptotic; Blood Banks; Blood Cells; Blood Platelets; Blood Transfusion; Cells; Clinical; Collaborations; Color; Complex; Computer software; Consultations; Core Facility; Cryoultramicrotomy; Cytometry; Effectiveness; Equipment; Flow Cytometry; Frequencies; Frozen Sections; Goals; Hematopoietic; Homing; Image; Image Cytometry; In Situ; Label; Laser Scanning Cytometry; Licensing; Liver; Lung; Magnetism; Maintenance; Modernization; Molecular; Organ; Outcome; Pathway interactions; Patients; Platelet Transfusion; Platelet aggregation; Population; Productivity; Program Research Project Grants; Proteins; Pulmonary Inflammation; Reporter; Research; Research Project Grants; Resources; Rest; S100A8 gene; Sorting; Source; Spleen; Stromal Cells; Suspensions; System; Thick; Thinness; Three-Dimensional Imaging; Time; Tissues; Titrations; Transfusion; Transplant Recipients; Transplantation; blood product; confocal imaging; cost effective; cryostat; design; experimental study; hematopoietic cell transplantation; improved; instrument; interest; member; mesenchymal stromal cell; neutrophil; novel strategies; particle; programs; quantitative imaging; transfusion medicine

Project Summary: Cytometry and Imaging Core The key objective of the Cytometry and Imaging Core is to enhance the overall productivity of the Program by providing a cost-effective, centralized, essential resource for the use of laser scanning cytometry (LaSC), 3D imaging, cellular pre-enrichment, and flow cytometry. The Core will provide i) a centralized resource of LaSC for all 3 program projects; ii) a centralized, cost-effective resource of flow cytometry for Project 1 and 2 to access two flow cytometry analyzers, a 16-parameter flow cytometer Attune NxT and an 8-parameter flow cytometer Canto II; iii) a centralized resource of 3D confocal imaging for all 3 program projects; iv) a resource of expert advice and consultation in the design and analysis of complex, multicolor flow cytometry and LaSC experiments including FlowJo software licenses in flow cytometry analysis and iCys cytometric analysis software (CompuCyte) in LaSC for all 3 program projects; and v) a centralized resource of cell enrichment to save time and money for Project 1 and 2. By providing a cost-effective, centralized resource for research projects to use LaSC, 3D imaging, cellular pre-enrichment, and flow cytometry, the Cytometry and Imaging Core will help further the Programs goals of understanding the molecular and cellular mechanisms underlying the effect and effectiveness of blood transfusion in the peri-transplant setting.

项目摘要：细胞计数和成像核心 细胞计数和成像核心的主要目标是通过以下方式提高程序的整体生产力 为激光扫描细胞仪（LaSC）的使用提供了一个具有成本效益的集中式基本资源， 成像、细胞预富集和流式细胞术。核心将提供i）LaSC的集中资源， 所有3个计划项目; ii）为项目1和2提供集中的、具有成本效益的流式细胞术资源， 流式细胞仪分析仪、16参数流式细胞仪Attune NxT和8参数流式细胞仪Canto II; iii） 所有3个项目的3D共聚焦成像的集中资源; iv）专家建议资源， 在复杂的流式细胞术和LaSC实验的设计和分析方面提供咨询，包括 FlowJo流式细胞仪分析软件和iCys流式细胞仪分析软件（CompuCyte）许可证， LaSC用于所有3个计划项目;以及v）集中的细胞富集资源，以节省时间和金钱， 项目1和2。通过为研究项目提供具有成本效益的集中资源来使用LaSC，3D 成像、细胞预富集和流式细胞术，细胞计数和成像核心将有助于进一步 课程的目标是了解影响的分子和细胞机制， 输血在围移植期的有效性。