Cytometry Core

项目摘要：细胞仪核心 细胞学核心的主要目标是通过提供一个 具有成本效益的集中化资源，用于基于抗体的细胞预富集、后续流程 基于细胞术的分析和纯化，用于2D和3D成像的薄和全/厚冰冻切片，以及 定量成像细胞术。核心将提供1)作为主力的8参数流式细胞仪 用于所有三个项目中建议的大多数流式细胞仪实验的分析仪器，2)a 用于定量成像细胞术的激光扫描细胞术(LASC)的集中式、经济高效的资源 在所有三个项目中提出的分析，3)制备厚冻组织切片和3D共聚焦成像，4)a) 复杂、多色流式细胞仪设计和分析方面的专家建议和咨询资源 以及LASC实验，包括用于流式细胞仪分析和ICYS细胞仪的FlowJo软件许可证 用于LASC分析的分析软件(CompuCyte)，以及5)用于蜂窝玫瑰花结和 基于磁粉的策略在随后的流式细胞术之前预富集感兴趣的细胞 基于分析和/或分类。通过为基于抗体的细胞提供经济高效的集中化资源 基于预富集和随后的细胞术(流式细胞术和LASC)的分析和纯化， 细胞学核心将有助于进一步了解控制功能的途径的计划目标 输注的骨髓、脐带血和外周血细胞。

Project Summary: Cytometry core The key objective of the Cytometry Core is to enhance the overall productivity of the Program by providing a cost-effective, centralized resource for the use of antibody based cellular pre-enrichment, subsequent flow cytometry based analysis and purification, thin and whole/thick frozen sections for 2D and 3D imaging, and quantitative imaging cytometry. The core will provide 1) the 8 parameter flow cytometer that is the workhorse analytical instrument for the majority of flow cytometry experiments proposed in all three projects, 2) a centralized, cost-effective resource for the laser scanning cytometry (LaSC) for quantitative imaging cytometry analysis proposed in all three projects, 3) preparation of thick cryostat tissue sections and 3D confocal imaging, 4) a resource for expert advice and consultation in the design and analysis of complex, multicolor flow cytometry and LaSC experiments including FlowJo software licenses for flow cytometry analysis and iCys cytometric analysis software (CompuCyte) for LaSC analysis, and 5) magnets and reagents for cellular rosetting and magnetic-particle based strategies for pre-enrichment of cells of interest prior to subsequent flow cytometry based analysis and/or sorting. By providing a cost-effective, centralized resource for antibody based cellular pre-enrichment and subsequent cytometry (flow cytometry and LaSC) based analysis and purification, the Cytometry core will help further the Programs goals of understanding the pathways which control the function of transfused bone marrow, cord blood, and peripherally derived cells.