Resource Core 2 - Primate/rodent Optics Core (POC)

资源核心 2 - 灵长类动物/啮齿类动物光学核心 (POC)

• 批准号: 10687140
• 负责人: Ailey Crow
• 金额: $ 23.77万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-17 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10687140
• 关键词: Address; Adult; Area; Attention; Back; Behavior; Behavioral; Brain; Brain region; Categories; Cells; Cognition; Collaborations; Color; Communities; Computer Analysis; Crows; Dedications; Development; Devices; Diameter; Doctor of Philosophy; Dorsal; Educational process of instructing; Esthesia; Experimental Designs; Fiber; Fluorescence; Genetic; Goals; Head; Image; Imaging technology; Investigation; Joints; Lighting; Maps; Measures; Methods; Microscope; Modernization; Motor; Mus; Nature; Neocortex; Neurons; Noise; Operative Surgical Procedures; Opsin; Optics; Outcome; Population; Positioning Attribute; Preparation; Primates; Process; Publishing; Research Project Grants; Resolution; Resources; Rest; Rodent; Science; Scientist; Sensory; Signal Transduction; Site; Specific qualifier value; Speed; Structure-Activity Relationship; Supervision; Systems Development; Technology; Training; Translating; United States National Institutes of Health; Validation; Visit; Work; Writing; brain based; cellular imaging; design; experience; improved; in vivo; interest; lens; member; neocortical; neural; neural circuit; new technology; next generation; nonhuman primate; novel; optogenetics; portability; ratiometric; real world application; sensor; technological innovation; theories; tool; two-photon; virtual

Resource Core 2 - Primate/rodent Optics Core (POC) Leads: Ailey Crow PhD and Sean Quirin PhD Project Summary The Primate/rodent Optics Core (POC) involves the guided use and ongoing refinement of continuously- modernized optical technologies across projects. We recognize the need now to 1) build optical strategies for dedicated U19 team application, and 2) continuously adapt and modernize to keep up with the fast-moving field over the course of the 5-year overall project. The Core will meet this project-specific goal, to address the many technologically-intensive optical questions that will continuously arise across projects, and to capitalize on new technological opportunities to ensure that U19 team maintains capability to push the envelope. The POC will develop, validate, and implement (in the real-world setting of the U19 team projects/collaborations), multiple next-generation technologies for studying neural circuit structure-function relationships combined with optogenetic control. These include our novel simultaneous high-speed multi-site quantification of genetically- specified neural activity traffic across the adult mammalian (rodent or primate) brain-- suitable for measuring experience-triggered joint activity relationships among multiple brainwide projections and cell populations. These also include our advancement and cross-species disseminati0n of wide-field high-resolution activity imaging and our two-photon multiple-single-cell imaging and stimulation technology. We pay particular attention to integrative methods for crossing scales of observation and manipulation, from single neuron- resolution all the way to brainwide analysis during behavior. Results from U19 Team implementation, relating to optical figures of merit, including signal-to-noise, aberrations, and speed, will be fed back to the Core development process led by Dr. Sean Quirin, Dr. Ailey Crow and the rest of the POC staff, under supervision of PD/PI Karl Deisseroth, in a tight closed-loop workflow guided by real-world application, a key opportunity for fundamental science itself.

资源核心2-灵长类/啮齿动物光学核心(POC) 线索：艾莉·克罗博士和肖恩·奎林博士 项目摘要 灵长类/啮齿动物光学核心(POC)涉及引导使用和持续改进- 跨项目采用现代化的光学技术。我们认识到现在有必要1)为以下目标制定光纤战略 专用U19团队应用，2)不断适应和现代化，以跟上快速发展的领域 在为期5年的整个项目过程中。核心将满足这一特定于项目的目标，以解决许多 技术密集型光学问题将在项目中不断出现，并利用新的 确保U19团队保持突破极限能力的技术机会。PoC将 开发、验证和实施(在U19团队项目/协作的真实环境中)、多个 结合神经电路结构-功能关系研究的下一代技术 光遗传控制。这些包括我们的新的同时高速多点定量的遗传- 成年哺乳动物(啮齿动物或灵长类)大脑中特定的神经活动流量--适合测量 经验触发的多个全脑投射和细胞群体之间的联合活动关系。 这些还包括我们在广域高分辨率活动方面的进展和跨物种传播 成像和我们的双光子多单细胞成像和刺激技术。我们付的钱特别多 注意跨越观察和操作尺度的综合方法，从单个神经元到 在行为过程中，从解决问题到全脑分析。U19团队实施的结果，相关 光学品质因数，包括信噪比、像差和速度，将反馈到核心 由Sean Quirin博士、Ailey Crow博士和POC其他工作人员领导的开发过程，在 PD/PI Karl Deisseroth，在以真实世界应用程序为指导的紧密闭环工作流程中， 基础科学本身。