Resource Core 2 - Primate/rodent Optics Core (POC)

资源核心 2 - 灵长类动物/啮齿类动物光学核心 (POC)

• 批准号: 10047730
• 负责人: Ailey Crow
• 金额: $ 22.45万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-17 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10047730
• 关键词: Address; Adult; Area; Attention; Back; Base of the Brain; Behavior; Behavioral; Brain; Brain region; Caliber; Categories; Cells; Cognition; Collaborations; Color; Communities; Computer Analysis; Crows; Development; Devices; Doctor of Philosophy; Dorsal; Ensure; Esthesia; Experimental Designs; Fiber; Fluorescence; Genetic; Goals; Head; Image; Imaging technology; Investigation; Joints; Lighting; Measures; Methods; Microscope; Modernization; Motor; Mus; Nature; Neocortex; Neurons; Noise; Operative Surgical Procedures; Opsin; Optics; Outcome; Population; Positioning Attribute; Preparation; Primates; Process; Publishing; Research Project Grants; Resolution; Resources; Rest; Rodent; Science; Scientist; Sensory; Signal Transduction; Site; Specific qualifier value; Speed; Structure-Activity Relationship; Supervision; Systems Development; Technology; Training; Translating; United States National Institutes of Health; Validation; Visit; Work; Writing; base; cellular imaging; design; experience; improved; in vivo; interest; lens; member; neural circuit; next generation; nonhuman primate; novel; optogenetics; portability; ratiometric; real world application; relating to nervous system; sensor; technological innovation; theories; tool; two-photon; virtual

Resource Core 2 - Primate/rodent Optics Core (POC) Leads: Ailey Crow PhD and Sean Quirin PhD Project Summary The Primate/rodent Optics Core (POC) involves the guided use and ongoing refinement of continuously- modernized optical technologies across projects. We recognize the need now to 1) build optical strategies for dedicated U19 team application, and 2) continuously adapt and modernize to keep up with the fast-moving field over the course of the 5-year overall project. The Core will meet this project-specific goal, to address the many technologically-intensive optical questions that will continuously arise across projects, and to capitalize on new technological opportunities to ensure that U19 team maintains capability to push the envelope. The POC will develop, validate, and implement (in the real-world setting of the U19 team projects/collaborations), multiple next-generation technologies for studying neural circuit structure-function relationships combined with optogenetic control. These include our novel simultaneous high-speed multi-site quantification of genetically- specified neural activity traffic across the adult mammalian (rodent or primate) brain-- suitable for measuring experience-triggered joint activity relationships among multiple brainwide projections and cell populations. These also include our advancement and cross-species disseminati0n of wide-field high-resolution activity imaging and our two-photon multiple-single-cell imaging and stimulation technology. We pay particular attention to integrative methods for crossing scales of observation and manipulation, from single neuron- resolution all the way to brainwide analysis during behavior. Results from U19 Team implementation, relating to optical figures of merit, including signal-to-noise, aberrations, and speed, will be fed back to the Core development process led by Dr. Sean Quirin, Dr. Ailey Crow and the rest of the POC staff, under supervision of PD/PI Karl Deisseroth, in a tight closed-loop workflow guided by real-world application, a key opportunity for fundamental science itself.

资源核心2 -灵长类动物/啮齿类动物光学核心（POC） 负责人：艾利克罗博士和肖恩奎林博士 项目摘要 灵长类动物/啮齿动物光学核心（POC）涉及指导使用和持续改进的连续- 在项目中实现光学技术的现代化。我们认识到现在需要1）建立光学战略， 专门的U19团队应用程序，以及2）不断适应和现代化，以跟上快速发展的领域 在整个五年计划的过程中。核心将满足这一项目特定的目标，以解决许多 技术密集型的光学问题，将不断出现在项目中，并利用新的 技术机会，以确保U19队保持能力，推动信封。POC将 开发、验证和实施（在U19团队项目/协作的现实环境中）， 研究神经回路结构-功能关系的下一代技术， 光遗传控制这些包括我们的新的同时高速多位点定量的遗传- 成年哺乳动物（啮齿动物或灵长类动物）大脑中特定的神经活动流量-适用于测量 经验触发多个全脑投射和细胞群之间的联合活动关系。 这些也包括我们的进步和跨物种传播的宽场高分辨率活动 成像和我们的双光子多单细胞成像和刺激技术。我们特别 注意观察和操作的综合方法，从单个神经元- 从解决问题一直到行为过程中的全脑分析。U19团队实施的结果， 光学品质因数，包括信噪比、像差和速度，将反馈到核心 开发过程由Sean Quirin博士、艾利·克罗博士和其他POC工作人员领导，并在以下人员的监督下进行 PD/PI Karl Deisseroth，在由实际应用指导的紧密闭环工作流程中， 基础科学本身。