Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
基本信息
- 批准号:10704035
- 负责人:
- 金额:$ 52.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-12 至 2024-07-31
- 项目状态:已结题
- 来源:
- 关键词:Afferent NeuronsAnimal ModelAnimalsAplysiaAxonBiologicalBiologyCell Culture SystemCell NucleusCellsDataExposure toFunctional disorderGene ExpressionGenesGenetic TranscriptionGillsHourIn VitroInvertebratesKinesinKnowledgeLearningMediatingMemoryMemory DisordersMessenger RNAMicrodissectionMolecularMolecular BiologyMolecular MotorsMotor NeuronsNeuronsNeurotransmittersPathway AnalysisPerfusionPhaseProtein BiosynthesisProteinsRNARNA TransportReflex actionRegulationRoleSerotoninSignal PathwaySnailsSpecificitySynapsesSynaptic TransmissionSystemTestingTranscriptional ActivationVertebratesWithdrawalanalogdifferential expressionexperimental studyinsightinterdisciplinary approachlong term memorymarineneural circuitnovelpostsynapticpostsynaptic neuronspresynapticpresynaptic neuronsprogramsreconstitutionrecruitsea slugsynaptogenesistherapeutic developmenttranscriptometranscriptome sequencingtranscriptomics
项目摘要
PROJECT SUMMARY
Long-term memory (LTM) storage requires remodeling of pre-existing synapses and formation of new
ones. While the necessity of transcription in the temporal phases of LTM such as formation and persistence
has been described, we lack comprehensive information on how gene expression changes in a neural circuitry
mediate the formation and persistence of LTM. In any of the animal models of learning, we do not know what
components of the transcriptomes are recruited specifically in pre- or post-synaptic neurons for mediating the
formation and persistence of LTM. Particularly we know very little about the gene expression changes
required for the persistence of LTM. Lack of this knowledge is a critical barrier for deciphering the molecular
underpinnings of synapse specificity and LTM. The central hypothesis of this proposal is that coordinated
regulation of the pre- and the post-synaptic neuronal transcriptome mediate the formation and
persistence of synapse specific LTM. We propose to test this hypothesis using the well-characterized
neural circuitry of gill-withdrawal reflex (GWR) of the marine snail, Aplysia californica. Specifically, using a
modified bifurcated sensory neuron-motor neuron culture, we will assess the changes in the subcellular
transcriptome of pre-synaptic sensory neurons and post-synaptic motor neurons during the formation and
persistence of the synapse specific LTM. Furthermore, the role of molecular motor kinesin mediated transport
of RNAs from the cell body to synapses in synapse specific LTM will be determined. We anticipate that these
experiments will facilitate the decoding of the gene expression program for the formation and persistence of
LTM in components of a defined neural circuitry. Furthermore, our studies are expected to produce a ground-
breaking impact on the basic biology of synapse specific LTM as well as to facilitate identification of novel
candidates for the development of therapeutics for memory disorders.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Sathyanarayanan V Puthanveettil其他文献
Sathyanarayanan V Puthanveettil的其他文献
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{{ truncateString('Sathyanarayanan V Puthanveettil', 18)}}的其他基金
Assessing effects of manipulation of expression of kinesins in a mouse modelof Alzheimer's disease
评估阿尔茨海默病小鼠模型中驱动蛋白表达的操纵效果
- 批准号:
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- 资助金额:
$ 52.3万 - 项目类别:
Assessing the role of circRNAs in memory consolidation
评估 circRNA 在记忆巩固中的作用
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10425435 - 财政年份:2021
- 资助金额:
$ 52.3万 - 项目类别:
Assessing the role of circRNAs in memory consolidation
评估 circRNA 在记忆巩固中的作用
- 批准号:
10676541 - 财政年份:2021
- 资助金额:
$ 52.3万 - 项目类别:
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评估 circRNA 在记忆巩固中的作用
- 批准号:
10307872 - 财政年份:2021
- 资助金额:
$ 52.3万 - 项目类别:
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
- 批准号:
9896348 - 财政年份:2019
- 资助金额:
$ 52.3万 - 项目类别:
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
- 批准号:
10456810 - 财政年份:2019
- 资助金额:
$ 52.3万 - 项目类别:
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
- 批准号:
10609651 - 财政年份:2019
- 资助金额:
$ 52.3万 - 项目类别:
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
- 批准号:
10224786 - 财政年份:2019
- 资助金额:
$ 52.3万 - 项目类别:
Transcriptomic Mechanisms of Formation and Persistence of Synapse Specific Long-Term Memory
突触特异性长期记忆形成和持续的转录组机制
- 批准号:
10017327 - 财政年份:2019
- 资助金额:
$ 52.3万 - 项目类别:
Dynein-Dynactin Complex Mediated Retrograde Transport during Long-term Memory Storage
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10626279 - 财政年份:2018
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$ 52.3万 - 项目类别:
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