Project I: Systems analysis of tumor-stroma interactions in brain metastasis

项目一：脑转移中肿瘤-基质相互作用的系统分析

• 批准号: 10705775
• 负责人: JOAN MASSAGUE
• 金额: $ 49.56万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10705775
• 关键词: Adenocarcinoma Cell; Alzheimer' s Disease; Apoptosis; Astrocytes; Basement membrane; Binding; Blood Vessels; Brain; Breast Cancer Cell; Cancer Patient; Cell Communication; Cells; Clinical; Communication; Complex; Disease associated microglia; Disseminated Malignant Neoplasm; ERBB2 gene; Exclusion; Extracellular Matrix Proteins; Factor Analysis; Gene Expression; Genes; Genetic; Genetic Transcription; Goals; Growth; Homeostasis; Immune; Immune system; Immunologist; Label; Laminin; Lead; Learning; Logic; Lung Adenocarcinoma; Malignant Neoplasms; Malignant neoplasm of lung; Mediating; Mediator; Memorial Sloan-Kettering Cancer Center; Metastatic malignant neoplasm to brain; Microglia; Modeling; Morbidity - disease rate; Natural Immunity; Nature; Neoplasm Metastasis; Neural Cell Adhesion Molecule L1; Patients; Phenotype; Physicians; Primary Neoplasm; Research; Research Personnel; Resistance; Sampling; Shapes; Signal Transduction; System; Systems Analysis; Systems Biology; Testing; Therapeutic Intervention; Tissues; Transcriptional Regulation; autocrine; brain cell; brain shape; cancer cell; cell type; cerebral capillary; contextual factors; differential expression; knowledge of results; lung colonization; malignant breast neoplasm; mortality; mouse model; multiple omics; pharmacologic; prevent; programs; response; single cell analysis; single-cell RNA sequencing; spatial relationship; spatiotemporal; therapeutic development; therapeutic evaluation; triple-negative invasive breast carcinoma; tumor; tumor growth; unpublished works

Project I. Systems Analysis of Tumor-Stroma Interactions in Brain Metastasis Experimental Lead: Massagué Computational Lead: Pe’er PROJECT SUMMARY The overall goal of this project is to apply systems-level computational approaches to mouse models and clinical samples to unravel strategies that metastatic cancer cells employ to colonize the brain. Brain metastasis is a major cause of morbidity and mortality in breast and lung cancer patients. The Massagué Lab pioneered studies to identify mediators of brain metastasis and relevant cell-cell interactions, and are subjecting this problem to systems-level analysis with the Pe’er Lab. We recently found that brain metastatic lesions of triple-negative breast cancer (TNBC), HER2+ breast cancer (HER2BC), and lung adenocarcinoma (LUAD) in mouse models and patient samples display remarkable differences in the spatial relationship between cancer cells and the host tissue. TNBC and LUAD cells spread along brain capillaries via L1CAM, forming perivascular colony networks that intermingle with microglia and astrocytes. In sharp contrast, HER2BC cells colonize the brain by forming compact spheroidal colonies that exclude brain parenchymal cells. High expression of specific extracellular matrix proteins by HER2BC cells drives this spheroidal growth. Notably, the perivascular and spheroidal colonies trigger distinct disease-associated microglia (DAM) innate immunity stages previously defined in Alzheimer’s disease. Aim 1 is to elucidate the DAM regulatory mechanisms in metastasis-associated microglia. We will resolve transcriptional regulation of the homeostasis-to-DAM transition in brain metastasis by analyzing single-cell multiomic profiles of metastasis-associated microglia. We will dissect how cancer cells trigger and modulate DAM responses by testing the hypothesis that HER2BC apoptosis triggers stage 2 DAM, whereas enhanced survival of TNBC retains stage 1 DAM. We will identify drivers of TNBC-intrinsic resistance to apoptosis by computationally guided search for autocrine pro-survival signaling in TNBC cells. We will determine how stage 1 DAM supports tumor growth. We will seek to connect metastasis-associated microglia to activated microglia states across contexts by supervised gene set analysis. Aim 2 is to define spatiotemporal progression and multicellular communication in brain metastasis by a systems-level analysis. We and others have shown the engagement of multiple cell types besides microglia to support brain metastasis. These interactions call for a comprehensive interrogation of the various ensembles of multicellular communication that shape brain metastasis. We will leverage our LUAD-to-brain metastasis models to study “what” cell types and programs constitute multicellular communication ensembles. We will analyze intact metastatic colonies to learn “where” the cells are localized and which programs are differentially expressed. We will unravel “how” the cells and programs shape metastasis by computationally inferring the formation and impact of multicellular ensembles from snapshots of metastatic colonies. We will perturb the inferred ensembles to test therapeutic intervention paradigms. The resulting knowledge, enriched with the input of the CSBC Research Center immunologists and physicians, will ultimately guide the development of therapeutic interventions to target brain metastases. 1

项目一：脑转移中肿瘤-基质相互作用的系统分析