评估诱导低温耐受的新范式-猫眼课题宝

评估诱导低温耐受的新范式

批准号：

8243062

负责人：

Ram Devireddy

金额：

$18.5万

依托单位：

LOUISIANA STATE UNIV A&M COL BATON ROUGE

依托单位国家：

美国

项目类别：

财政年份：

2012

资助国家：

美国

项目状态：

已结题

起止时间：

2012-07-01 至 2014-06-30

来源：

https://reporter.nih.gov/project-details/8243062

关键词：

Adherence Adipocytes Adipose tissue Alkaline Phosphatase Allogenic Animals Antibodies Autologous Biochemical Biological Assay Cardiac Cell Survival Cell Therapy Cells Centrifugation Chemicals Chills Chondrocytes Clinical Colony-Forming Units Assay Colony-forming units Communities Cryopreservation Cryopreserved Cell Detection Development Digestion Dimethyl Sulfoxide Disease Dyspnea Effectiveness Fibroblasts Flow Cytometry Freezing Genes Goals HSPB1 gene Heart Heart Arrest Heat shock proteins Heat-Shock Response Hormonal Human Hypotension Hypoxia Immunoblotting Immunotherapy In Vitro Infusion procedures Kidney Failure Laboratories Leptin Link Molecular Chaperones Muscle Cells Nausea Neurologic Neurons Osteoblasts Osteocalcin Osteogenesis Pathway interactions Patients Physicians Plastics Process Protocols documentation RNA analysis Regimen Relative (related person)Reporting Reverse Transcriptase Polymerase Chain Reaction Safety Serum Specimen Stem cell transplant Stem cells Stimulus Surface Testing Tissues Toxic effect Translating Undifferentiated Western Blotting adiponectin adult stem cell base bone sialoprotein collagenase conditioning cost improved in vivo lipid biosynthesis mineralization novel oil red O osteogenic protein expression research study respiratory response standard of care

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DESCRIPTION (provided by applicant):The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs). PUBLIC HEALTH RELEVANCE: This proposal will determine the link between the expression of heat shock proteins and pre-freeze (control) and post-freeze (cryopreservation) potential (osteogenic and adipogenic) of human adipose tissue derived adult stem cells (ASCs).

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