Assessing a Novel Paradigm of Inducing Cryotolerance

评估诱导低温耐受的新范式

• 批准号: 8243062
• 负责人: Ram Devireddy
• 金额: $ 18.5万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8243062
• 关键词: Adherence; Adipocytes; Adipose tissue; Alkaline Phosphatase; Allogenic; Animals; Antibodies; Autologous; Biochemical; Biological Assay; Cardiac; Cell Survival; Cell Therapy; Cells; Centrifugation; Chemicals; Chills; Chondrocytes; Clinical; Colony-Forming Units Assay; Colony-forming units; Communities; Cryopreservation; Cryopreserved Cell; Detection; Development; Digestion; Dimethyl Sulfoxide; Disease; Dyspnea; Effectiveness; Fibroblasts; Flow Cytometry; Freezing; Genes; Goals; HSPB1 gene; Heart; Heart Arrest; Heat shock proteins; Heat-Shock Response; Hormonal; Human; Hypotension; Hypoxia; Immunoblotting; Immunotherapy; In Vitro; Infusion procedures; Kidney Failure; Laboratories; Leptin; Link; Molecular Chaperones; Muscle Cells; Nausea; Neurologic; Neurons; Osteoblasts; Osteocalcin; Osteogenesis; Pathway interactions; Patients; Physicians; Plastics; Process; Protocols documentation; RNA analysis; Regimen; Relative (related person); Reporting; Reverse Transcriptase Polymerase Chain Reaction; Safety; Serum; Specimen; Stem cell transplant; Stem cells; Stimulus; Surface; Testing; Tissues; Toxic effect; Translating; Undifferentiated; Western Blotting; adiponectin; adult stem cell; base; bone sialoprotein; collagenase; conditioning; cost; improved; in vivo; lipid biosynthesis; mineralization; novel; oil red O; osteogenic; protein expression; research study; respiratory; response; standard of care

DESCRIPTION (provided by applicant):The practical goals of this study will be the development and optimization of a serum free cryoprotection medium with minimum levels of cryoprotectants. We intend to pursue these studies using a hypothesis-driven, mechanism-based experimental approach, i.e., the viability of the cryopreserved adult stem cells (ASCs), in the undifferentiated, adipocyte- or osteoblast- differentiated states, can be improved by increasing the pre-freeze expression of heat shock proteins (HSPs). PUBLIC HEALTH RELEVANCE: This proposal will determine the link between the expression of heat shock proteins and pre-freeze (control) and post-freeze (cryopreservation) potential (osteogenic and adipogenic) of human adipose tissue derived adult stem cells (ASCs).

描述(由申请人提供)：这项研究的实际目标将是开发和优化一种无血清冷冻保护剂，其冷冻保护剂的水平最低。我们打算使用一种假设驱动的、基于机制的实验方法来进行这些研究，即，可以通过增加热休克蛋白(HSPs)的冷冻前表达来提高未分化、脂肪细胞或成骨细胞分化状态下冷冻保存的成体干细胞(ASCs)的活性。 公共卫生相关性：这项提案将确定热休克蛋白的表达与人类脂肪组织来源的成人干细胞(ASCs)冷冻前(对照)和冷冻后(冷冻)潜力(成骨和成脂)之间的联系。