Acquisition of an Oxford Nanopore sequencer for genomic analysis of a subterrestrial nematode

购买牛津纳米孔测序仪用于地下线虫的基因组分析

• 批准号: 10797580
• 负责人: John Russell Bracht
• 金额: $ 9.6万
• 依托单位: AMERICAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-07 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797580
• 关键词: Affect; American; Antibiotics; Apoptosis; Arginine; Astrocytes; Autoimmune; Autoimmune Diseases; Award; Caenorhabditis elegans; Cellular Stress; Development; Disease; Elements; Environment; Faculty; Family; Funding; Gene Expression; Genes; Genetic; Genetic Transcription; Genome; Genomics; Gold; Growth; Heat Stress Disorders; Heat-Shock Proteins 70; Heat-Shock Response; Human Pathology; Hypoxia; Investigation; Laboratories; Life; Link; Malignant Neoplasms; Maps; Measures; Methane; Molecular; Mutate; Nematoda; Nerve Degeneration; Neurodegenerative Disorders; Organism; Outsourcing; Parents; Pathway interactions; Phenotype; Play; Proteins; Publishing; Quantitative Reverse Transcriptase PCR; RNA; RNA Interference; Regulation; Research; Role; Sampling; Signal Transduction; Site; South Africa; South African; Starvation; Stress; Structure; Students; Technology; Testing; Therapeutic; Time; Toxin; Tumor stage; Tunicamycin; Underrepresented Students; Universities; Work; cell injury; cost; empowerment; environmental stressor; feeding; gene discovery; gene function; heat-shock factor 1; human disease; inhibitor; knock-down; nanopore; neoplastic; neurotrophic factor; novel; resilience; response; stress management; stress resilience; stressor; transcriptome sequencing; transcriptomics; undergraduate student

Abstract The supplemental funding will allow the purchase of an Oxford Nanopore sequencer to enable the Bracht laboratory to complete the aims of the parent award. The sequencing technology is critical given that 45 separate RNA samples must be sequenced and outsourcing the work is costly in both time and money. The Nanopore technology provides long, highly accurate reads enabling many exciting applications both for the parent award and beyond. The supplemental funding would provide a transformative technology, given there is currently no genomic sequencers available at American University; furthermore, there is no Nanopore technology available in the entire DC metro region containing at least five additional research universities beyond AU. This supplement would therefore fill a regional gap in technological availability, benefitting multiple universities and empowering the unique research conducted at American University: highly interdisciplinary, undergraduate-engaged and with a high engagement of under-represented students. Students and faculty alike would greatly benefit from the availability of a transformative Nanopore sequencer at AU. 1

摘要 补充资金将允许购买牛津纳米孔测序仪， Bracht实验室完成了母奖的目标。测序技术是 鉴于必须对45个独立的RNA样本进行测序， 在时间和金钱上都很昂贵。Nanopore技术提供长时间、高精度的读数 使许多令人兴奋的应用程序都为家长奖和超越。补充 资金将提供一种变革性的技术，因为目前还没有基因组 美国大学的测序仪;此外，没有纳米孔技术 在整个DC大都会地区提供，至少包含五所额外的研究型大学 超过了Au。因此，这一补充将填补技术供应方面的区域差距， 使多所大学受益，并使美国大学进行的独特研究 大学：高度跨学科，本科生参与和高参与度 代表性不足的学生学生和教师都将大大受益于可用性 一个变革性的纳米孔测序仪 1