Acquisition of an Oxford Nanopore sequencer for genomic analysis of a subterrestrial nematode

摘要 补充资金将允许购买牛津纳米孔测序仪，以实现 布拉赫特实验室完成了家长奖的目标。测序技术是 鉴于必须对45个单独的RNA样本进行测序，将工作外包是至关重要的 在时间和金钱上都很昂贵。纳米孔技术提供了长时间、高精度的读数 支持许多令人兴奋的应用程序，包括父级奖项和其他奖项。补充资料 资金将提供一种变革性的技术，因为目前还没有基因组 美国大学有测序仪；此外，没有纳米孔技术 在包含至少五所额外研究型大学的整个DC大都市区提供 超越AU。因此，这一补充将填补技术可获得性方面的区域差距， 使多所大学受益，并支持在美国进行的独特研究 大学：高度跨学科，本科生敬业度高，对 代表不足的学生。学生和教职员工都将从可用性中受益匪浅 在AU的一个变革性的纳米孔测序仪。 1

Abstract The supplemental funding will allow the purchase of an Oxford Nanopore sequencer to enable the Bracht laboratory to complete the aims of the parent award. The sequencing technology is critical given that 45 separate RNA samples must be sequenced and outsourcing the work is costly in both time and money. The Nanopore technology provides long, highly accurate reads enabling many exciting applications both for the parent award and beyond. The supplemental funding would provide a transformative technology, given there is currently no genomic sequencers available at American University; furthermore, there is no Nanopore technology available in the entire DC metro region containing at least five additional research universities beyond AU. This supplement would therefore fill a regional gap in technological availability, benefitting multiple universities and empowering the unique research conducted at American University: highly interdisciplinary, undergraduate-engaged and with a high engagement of under-represented students. Students and faculty alike would greatly benefit from the availability of a transformative Nanopore sequencer at AU. 1