Investigating the molecular basis of evolved stress resilience in a subterrestrial nematode

研究地下线虫进化的应激恢复能力的分子基础

• 批准号: 10438979
• 负责人: John Russell Bracht
• 金额: $ 42.52万
• 依托单位: AMERICAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-07 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10438979
• 关键词: Affect; Antibiotics; Apoptosis; Arginine; Astrocytes; Autoimmune; Autoimmune Diseases; Caenorhabditis elegans; Cellular Stress Response; Development; Disease; Elements; Environment; Family; Gene Expression; Genes; Genetic; Genetic Transcription; Genome; Genomics; Growth; Heat Stress Disorders; Heat-Shock Proteins 70; Heat-Shock Response; Human Pathology; Hypoxia; Investigation; Laboratories; Life; Link; Malignant Neoplasms; Measures; Methane; Molecular; Mutate; Nematoda; Nerve Degeneration; Neurodegenerative Disorders; Organism; Pathway interactions; Phenotype; Play; Proteins; Publishing; Quantitative Reverse Transcriptase PCR; RNA Interference; Regulation; Role; Signal Transduction; Site; South Africa; South African; Starvation; Stress; Structure; Testing; Therapeutic; Toxin; Tumor stage; Tunicamycin; Work; cell injury; environmental stressor; feeding; gene discovery; gene function; heat-shock factor 1; human disease; inhibitor; knock-down; neoplastic; neurotrophic factor; novel; repaired; resilience; response; stress management; stress resilience; stressor; transcriptome sequencing; transcriptomics

A critical and well-studied cellular stress response pathway, the Unfolded Protein Response (UPR), protects organisms against several stressors including heat, hypoxia, starvation, and toxins. Helping to repair cellular damage, the UPR can also trigger apoptosis if the stress is ongoing, severe, and unrecoverable. Therefore, proper regulation of this pathway is essential, particularly since its malfunction contributes to human pathologies including autoimmune disorders, cancer, and neurodegenerative diseases. The Bracht lab recently published the genome of a nematode, Halicephalobus mephisto, isolated from the deep terrestrial subsurface of South Africa, over a kilometer underground. This organism has adapted to a stressful environment: hot, hypoxic, and rich in methane. Therefore the organism displays a naturally evolved resilience to stresses that would normally cause lethality; we also found that its UPR pathway is a site where adaptation has occurred. We have confirmed that RNA Interference (RNAi) by feeding can be used to modulate gene expression in this organism, setting the stage for a molecular investigation of stress resilience. Aim 1. Test the hypothesis that ARMET/MANF is not just an inhibitor of UPR signaling in H. mephisto. A UPR signaling gene discovered in 2003, Arginine-Rich, Mutated in Early-stage Tumors / Mesencephalic Astrocyte derived Neurotrophic Factor (ARMET / MANF), remains mysterious. While its precise molecular function has proven elusive, we identified it as the second most highly upregulated gene under heat stress in H. mephisto. In this aim, we will perform analysis of the transcriptomic changes when ARMET / MANF is knocked down by RNAi. Aim 2. Test the hypothesis that HSF1 has acquired an expanded role in heat resilience in H. mephisto. Heat-shock factor 1 (HSF1) is a well-characterized, conserved transcriptional regulator of the heat response across metazoa. However, we identified the potential for this protein to regulate 75% of the genes through a helitron-driven expansion of its recognition site. This aim is structured to test this apparent re-wiring of the HSF1 regulatory network. Aim 3. Test the role of HeaT-Upregulated-Protein-1 (HTUP-1) in heat and tunicamycin resilience. HTUP-1 is the most upregulated gene on heat in H. mephisto and it is unlike any other known protein--no blast matches and no recognizable domains. We hypothesize that HTUP-1 is a novel modulator of the evolved UPR response in H. mephisto. To study HTUP-1 function, we will inactivate it by RNAi, measure growth phenotypes under heat or tunicamycin stress, verify knockdown by qRT-PCR, and then perform RNA-seq to examine the pathways affected. Aim 4. Construct multi-copy arrays of H. mephisto genes in C. elegans as a mechanism of heat resilience. Hsp70 genes are extremely well characterized. However, in H. mephisto we uncovered a new family of Hsp70 genes: Hspa15; here we propose to evaluate whether these genes can confer heat tolerance de novo by heterologous expression in C. elegans. Because C. elegans is not thermotolerant, any acquired heat tolerance will be easily detected in this genetic background.

未折叠蛋白反应（UPR）是一种重要的、研究得很充分的细胞应激反应途径