Regulation of dynamic actin networks during epithelial morphogenesis

上皮形态发生过程中动态肌动蛋白网络的调节

• 批准号: 10797655
• 负责人: Jeffrey D Hardin
• 金额: $ 13.3万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-13 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797655
• 关键词: 3-Dimensional; Actins; Actomyosin; Adherens Junction; Adhesiveness; Area; Award; Biological Models; Biophysics; Cadherins; Caenorhabditis elegans; Cell Shape; Cell surface; Cells; Complex; Computer software; Congenital Abnormality; Cytoskeleton; Data; Data Set; Embryo; Embryonic Development; Epithelial Cells; Epithelium; Event; F-Actin; Four-dimensional; Funding; Genetic; Goals; Human; Human Development; Hybrids; Image; Injury; Intercalated Cell; Intercellular Junctions; Interference Microscopy; Invaded; LIM Domain; Link; Machine Learning; Malignant Neoplasms; Mechanics; Microscopy; Modeling; Modernization; Morphogenesis; Movement; Optics; Organism; Parents; Pathway interactions; Phenotype; Polymers; Positioning Attribute; Process; Proteins; Regulation; Resolution; Self-Injurious Behavior; Shapes; Signal Pathway; Signal Transduction; Speed; System; Testing; Time; Tissues; United States National Institutes of Health; Work; alpha catenin; cancer cell; cell motility; data acquisition; digital; feature detection; genome editing; healing; human disease; in vivo; insight; mechanotransduction; movie; multidisciplinary; mutant; novel; open source; parent grant; polymerization; prevent; recruit; single molecule

Project Summary/Abstract Embryonic development requires the dynamic remodeling of epithelial sheets as the embryo transforms itself during morphogenesis. Embryonic epithelia are hybrid tissues: they must maintain their integrity as mechanically integrated cell sheets, connected by cell-cell junctions, yet their cells must also retain the ability to dynamically change shape and position. The actin cytoskeleton is central to these activities; cells must link actomyosin networks dynamically to the cell surface via junctional complexes, and they must dynamically polymerize actin networks during cell shape changes and cell migratory events. Our goal is to understand the dynamics of actin networks during rapid cell shape change and during directed cell rearrangement in embryos. Our goal is a multidisciplinary, integrated analysis of morphogenetic movements in embryos that unites detailed structural analysis, single-molecule biophysics, genetics, and dynamic in vivo analysis, in an "Angstroms to embryos" approach to morphogenesis. Upgrading our ability to image living embryos using high-numerical aperture DIC optics and modern USB3- based cameras controlled by the NIH-supported, open-source software MicroManager will allow us to extend our analyses by providing higher throughout, better resolution, and enhanced focal stability relevant to all aims of the parent award.

项目总结/摘要 胚胎发育需要上皮片层的动态重塑， 在形态发生过程中自我转化胚胎上皮细胞是杂交组织：它们必须 保持它们作为机械整合的细胞片层的完整性，通过细胞-细胞连接点连接， 但它们的细胞还必须保持动态改变形状和位置的能力。肌动蛋白 细胞骨架是这些活动的中心;细胞必须动态地将肌动球蛋白网络连接到细胞骨架。 细胞表面通过连接复合物，它们必须动态地连接肌动蛋白网络 在细胞形状变化和细胞迁移事件期间。我们的目标是了解 肌动蛋白网络在快速细胞形状变化和定向细胞重排过程中， 胚胎我们的目标是多学科的，综合分析的形态发生运动， 胚胎，结合详细的结构分析，单分子生物物理学，遗传学， 动态体内分析，在“Angiotoembryos”的方法形态发生。升级 我们使用高数值孔径DIC光学器件和现代USB3成像活胚胎的能力- 由NIH支持的开源软件MicroManager控制的基于相机将允许 通过提供更高的通量，更好的分辨率和增强的焦点，我们可以扩展我们的分析 稳定性与母公司奖项的所有目标相关。