Regulation of dynamic actin networks during epithelial morphogenesis

上皮形态发生过程中动态肌动蛋白网络的调节

• 批准号: 10797655
• 负责人: Jeffrey D Hardin
• 金额: $ 13.3万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-13 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10797655
• 关键词: 3-Dimensional; Actins; Actomyosin; Adherens Junction; Adhesiveness; Area; Award; Biological Models; Biophysics; Cadherins; Caenorhabditis elegans; Cell Shape; Cell surface; Cells; Complex; Computer software; Congenital Abnormality; Cytoskeleton; Data; Data Set; Embryo; Embryonic Development; Epithelial Cells; Epithelium; Event; F-Actin; Four-dimensional; Funding; Genetic; Goals; Human; Human Development; Hybrids; Image; Injury; Intercalated Cell; Intercellular Junctions; Interference Microscopy; Invaded; LIM Domain; Link; Machine Learning; Malignant Neoplasms; Mechanics; Microscopy; Modeling; Modernization; Morphogenesis; Movement; Optics; Organism; Parents; Pathway interactions; Phenotype; Polymers; Positioning Attribute; Process; Proteins; Regulation; Resolution; Self-Injurious Behavior; Shapes; Signal Pathway; Signal Transduction; Speed; System; Testing; Time; Tissues; United States National Institutes of Health; Work; alpha catenin; cancer cell; cell motility; data acquisition; digital; feature detection; genome editing; healing; human disease; in vivo; insight; mechanotransduction; movie; multidisciplinary; mutant; novel; open source; parent grant; polymerization; prevent; recruit; single molecule

Project Summary/Abstract Embryonic development requires the dynamic remodeling of epithelial sheets as the embryo transforms itself during morphogenesis. Embryonic epithelia are hybrid tissues: they must maintain their integrity as mechanically integrated cell sheets, connected by cell-cell junctions, yet their cells must also retain the ability to dynamically change shape and position. The actin cytoskeleton is central to these activities; cells must link actomyosin networks dynamically to the cell surface via junctional complexes, and they must dynamically polymerize actin networks during cell shape changes and cell migratory events. Our goal is to understand the dynamics of actin networks during rapid cell shape change and during directed cell rearrangement in embryos. Our goal is a multidisciplinary, integrated analysis of morphogenetic movements in embryos that unites detailed structural analysis, single-molecule biophysics, genetics, and dynamic in vivo analysis, in an "Angstroms to embryos" approach to morphogenesis. Upgrading our ability to image living embryos using high-numerical aperture DIC optics and modern USB3- based cameras controlled by the NIH-supported, open-source software MicroManager will allow us to extend our analyses by providing higher throughout, better resolution, and enhanced focal stability relevant to all aims of the parent award.

项目概要/摘要 胚胎发育需要上皮片作为胚胎的动态重塑 在形态发生过程中发生自身转变。胚胎上皮是混合组织：它们必须 保持其机械集成细胞片的完整性，通过细胞-细胞连接点连接， 然而，它们的细胞还必须保留动态改变形状和位置的能力。肌动蛋白 细胞骨架是这些活动的核心；细胞必须动态地将肌动球蛋白网络连接到 通过连接复合物形成细胞表面，并且它们必须动态聚合肌动蛋白网络 在细胞形状变化和细胞迁移事件期间。我们的目标是了解动态 细胞形状快速变化和定向细胞重排过程中的肌动蛋白网络 胚胎。我们的目标是对形态发生运动进行多学科综合分析 胚胎结合了详细的结构分析、单分子生物物理学、遗传学和 动态体内分析，采用“埃到胚胎”的形态发生方法。升级中 我们能够使用高数值孔径 DIC 光学器件和现代 USB3-对活体胚胎进行成像 基于由 NIH 支持的开源软件 MicroManager 控制的相机将允许 我们通过提供更高的通量、更好的分辨率和增强的焦距来扩展我们的分析 与家长奖励的所有目标相关的稳定性。