Regulation of dynamic actin networks during epithelial morphogenesis

项目摘要/摘要 胚胎发育需要作为胚胎的上皮片的动态重塑 在形态发生过程中自我转化。胚胎上皮是杂交组织：它们必须 保持它们作为机械集成的电池片的完整性，通过细胞-细胞连接连接， 然而，它们的细胞还必须保持动态改变形状和位置的能力。肌动蛋白 细胞骨架是这些活动的中心；细胞必须动态地将肌动蛋白网络连接到 细胞表面通过连接复合体，它们必须动态聚合肌动蛋白网络 在细胞形状改变和细胞迁移事件期间。我们的目标是了解 肌动蛋白在快速细胞形状改变和定向细胞重排过程中的网络 胚胎。我们的目标是对形态发生运动进行多学科的综合分析 结合了详细的结构分析、单分子生物物理学、遗传学和 活体动态分析，以“从胚胎到胚胎”的方法进行形态发生。升级 我们使用高数值孔径DIC光学器件和现代USB3成像活胚胎的能力- 由NIH支持的开源软件微管理器控制的基于摄像头的摄像头将允许 我们将通过提供更高的吞吐量、更好的分辨率和增强的焦点来扩展我们的分析 与家长奖项的所有目标相关的稳定性。

Project Summary/Abstract Embryonic development requires the dynamic remodeling of epithelial sheets as the embryo transforms itself during morphogenesis. Embryonic epithelia are hybrid tissues: they must maintain their integrity as mechanically integrated cell sheets, connected by cell-cell junctions, yet their cells must also retain the ability to dynamically change shape and position. The actin cytoskeleton is central to these activities; cells must link actomyosin networks dynamically to the cell surface via junctional complexes, and they must dynamically polymerize actin networks during cell shape changes and cell migratory events. Our goal is to understand the dynamics of actin networks during rapid cell shape change and during directed cell rearrangement in embryos. Our goal is a multidisciplinary, integrated analysis of morphogenetic movements in embryos that unites detailed structural analysis, single-molecule biophysics, genetics, and dynamic in vivo analysis, in an "Angstroms to embryos" approach to morphogenesis. Upgrading our ability to image living embryos using high-numerical aperture DIC optics and modern USB3- based cameras controlled by the NIH-supported, open-source software MicroManager will allow us to extend our analyses by providing higher throughout, better resolution, and enhanced focal stability relevant to all aims of the parent award.