Structure and Specificity of Restriction-Modification (R-M) Systems

摘要 在NIGMS R35-GM131780的母公司授权下，标题为“限制-修饰的结构和特异性” (R-M)系统“，本申请是一项补充申请，以更换已被 已停产，不再由供应商提供支持和服务。所有进行的结构研究 在父母的资助下，它们严重依赖于蛋白质纯化--这是X光检查的必要前提 结晶学和低温电子显微镜。申请的是带有微型套件的Akta Pure 25 M系统，该套件将为1) 完全由供应商提供支持和服务，2)允许对蛋白质的纯化进行监控 一个波长，这很重要，因为父母拨款的目标是以DNA和RNA为中心的 结合蛋白，以及3)允许在微尺度上纯化蛋白质，这是必不可少的，特别是作为 母公司赠款的目标继续朝着低温新兴市场的方向发展。

Abstract Under the parent grant NIGMS R35-GM131780, titled “Structure and specificity of restriction-modification (R-M) systems”, this application is a supplemental request to replace an aged FPLC system that has been discontinued and is no longer supported and serviced by the vendor. All of the structural studies performed under the parent grant are heavily reliant on protein purification – a necessary prerequisite for X-ray crystallography and cryo-EM. The request is for an AKTA pure 25 M system with a Micro kit that will be 1) fully supported and serviced by the vendor, 2) allow the purification of proteins to be monitored at more than one wavelength, which is important because the aims of the parent grant are centered on DNA and RNA binding proteins, and 3) permit the purification of proteins on a microscale, which is essential, especially as the aims of the parent grant continue to grow in the direction of cryo-EM.