Structure and Specificity of Restriction-Modification (R-M) Systems

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Abstract Under the parent grant NIGMS R35-GM131780, titled “Structure and specificity of restriction-modification (R-M) systems”, this application is a supplemental request to replace an aged FPLC system that has been discontinued and is no longer supported and serviced by the vendor. All of the structural studies performed under the parent grant are heavily reliant on protein purification – a necessary prerequisite for X-ray crystallography and cryo-EM. The request is for an AKTA pure 25 M system with a Micro kit that will be 1) fully supported and serviced by the vendor, 2) allow the purification of proteins to be monitored at more than one wavelength, which is important because the aims of the parent grant are centered on DNA and RNA binding proteins, and 3) permit the purification of proteins on a microscale, which is essential, especially as the aims of the parent grant continue to grow in the direction of cryo-EM.