Development of MS2045 for inhibition of Zika methyltransferase
开发用于抑制寨卡病毒甲基转移酶的 MS2045
基本信息
- 批准号:10645958
- 负责人:
- 金额:$ 25.35万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-02 至 2024-12-31
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAdenineAdultAffinityAmericasAmino AcidsAntiviral AgentsAttenuatedBindingBinding SitesBiological AssayBiophysicsCellsChemistryCrystallizationDefectDengue VirusDevelopmentFlavivirusGoalsHIVHealthHepatitis CHumanIn VitroLeadLife Cycle StagesLinkMeasuresMethylationMethyltransferaseMicrocephalyMutationNewborn InfantNonstructural ProteinPathogenicityPathologyPatientsPharmaceutical ChemistryProcessRNARNA CapsRNA methylationStructureStructure-Activity RelationshipSyndromeTestingUgandaVaccinesVero CellsViralVirusVirus ReplicationWest Nile virusZIKAZIKV infectionZika Virusanalogantiviral drug developmentarthropod-bornebasechemical synthesiscofactordesignefficacy evaluationfuture outbreakhuman pathogeninhibitormembernovelpathogenscaffoldstructural biologyvaccine developmentviral RNAvirology
项目摘要
Project Summary
The Zika virus (ZIKV) is a member of the Flavivirus genus that includes other arthropod-borne human pathogens
such as dengue virus and West Nile virus, among others. ZIKV’s link to microcephaly in newborn infants and the
Guillan-Barré syndrome in adults has invigorated measures to develop a vaccine, as well as efforts to develop
antivirals based on targeting enzymatic activities central to the life cycle and survival of ZIKV. One such
enzymatic activity is encoded by the methyltransferase (MTase) domain, located at the N-terminus of the
nonstructural protein NS5. Taking a structure informed approach, we have succeeded in identifying a novel
“lead-like” compound (MS2045) for the inhibition of ZIKV’s NS5 MTase activity and for blocking its replication.
MS2045 provides a basis for further chemistry and the development of even more potent inhibitors. In aim 1,
we will a) design MS2045 analogs with the capacity to establish specific interactions with unique amino acids of
ZIKV MTase as a means to provide additional selectivity against the human RNA 5’-cap MTases; b) chemically
synthesize these analogs and produce them to a purity of 95% for in vitro and cell-based assays, and for
structural studies. In aim 2, we will a) perform biophysical assays to assess the ability of these analogs to
selectively bind the ZIKV NS5-MTase as compared to the human RNA 5’-cap MTases and test their ability to
inhibit RNA methylation; b) test these analogs in viral cell-based assays to assess their efficacy in blocking viral
replication; c) determine structures ZIKV NS5-Mtase with select analogs for additional, iterative rounds of
structure activity relationships (SARs). Collectively, these studies will help to identify analogs of MS2045 that
can be potentially developed into potent and selective inhibitors of NS5-MTase from ZIKV (and other pathogenic
flaviviruses)
项目摘要
寨卡病毒(ZIKV)是黄病毒属的成员,包括其他节肢动物传播的人类病原体
例如登革热病毒和西尼罗河病毒等。ZIKV与新生儿小头畸形症的联系
成年人的吉兰-巴雷综合征已经加快了开发疫苗的措施,
基于靶向对ZIKV的生命周期和存活至关重要的酶活性的抗病毒药。一个这样
酶活性由甲基转移酶(MTase)结构域编码,其位于酶的N-末端。
非结构蛋白NS 5以结构知情的方法,我们已经成功地确定了一个新的
本发明涉及“铅样”化合物(MS 2045)用于抑制ZIKV的NS 5 MTase活性和阻断其复制的用途。
MS 2045为进一步的化学和开发更有效的抑制剂提供了基础。在目标1中,
我们将a)设计MS 2045类似物,其具有与以下氨基酸建立特异性相互作用的能力:
ZIKV MTase作为提供针对人RNA 5 '-帽MTase的额外选择性的手段; B)化学上
合成这些类似物并将它们生产到95%纯度,用于体外和基于细胞的测定,以及用于
结构研究。在目标2中,我们将a)进行生物物理测定以评估这些类似物的能力,
与人RNA 5 '-帽MT酶相比,ZIKV NS 5-MT酶选择性结合ZIKV NS 5-MT酶,并测试它们与人RNA 5'-帽MT酶结合的能力。
抑制RNA甲基化; B)在基于病毒细胞的测定中测试这些类似物,以评估它们阻断病毒的功效
c)用选择的类似物确定ZIKV NS 5-Mtase的结构,用于另外的迭代轮的复制;
构效关系(SAR)。总的来说,这些研究将有助于鉴定MS 2045的类似物,
可以潜在地开发成来自ZIKV(和其他致病性病毒)的NS 5-MTase的有效和选择性抑制剂。
黄病毒)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ANEEL K. AGGARWAL其他文献
ANEEL K. AGGARWAL的其他文献
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{{ truncateString('ANEEL K. AGGARWAL', 18)}}的其他基金
Structure and Specificity of Restriction-Modification (R-M) Systems
限制性修饰(R-M)系统的结构和特异性
- 批准号:
10470890 - 财政年份:2019
- 资助金额:
$ 25.35万 - 项目类别:
Structure and Specificity of Restriction-Modification (R-M) Systems
限制性修饰(R-M)系统的结构和特异性
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10241952 - 财政年份:2019
- 资助金额:
$ 25.35万 - 项目类别:
Structure and Specificity of Restriction-Modification (R-M) Systems
限制性修饰(R-M)系统的结构和特异性
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10686907 - 财政年份:2019
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$ 25.35万 - 项目类别:
Structure and Specificity of Restriction-Modification (R-M) Systems
限制性修饰(R-M)系统的结构和特异性
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10797690 - 财政年份:2019
- 资助金额:
$ 25.35万 - 项目类别:
Structure and Specificity of Restriction-Modification (R-M) Systems
限制性修饰(R-M)系统的结构和特异性
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10727038 - 财政年份:2019
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$ 25.35万 - 项目类别:
Structure and Specificity of Restriction-Modification (R-M) Systems
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10599570 - 财政年份:2019
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