Agilent Complete 2D-LC-QTOF System

安捷伦完整的 2D-LC-QTOF 系统

基本信息

  • 批准号:
    10797808
  • 负责人:
  • 金额:
    $ 19.99万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-09-25 至 2024-06-30
  • 项目状态:
    已结题

项目摘要

Summary In the post-genomics era, a comprehensive analysis of “proteoforms” that arise from genetic variations and post-translational modifications (PTMs) is essential for understanding biological systems at a functional level and for dissecting complex molecular systems with consideration of individual variability for precision medicine. Top- down mass spectrometry (MS)-based proteomics that analyzes intact proteins is the most powerful method to comprehensively characterize proteoforms to decipher the PTM codes together with sequence variations. Although significant strides have been made recently in both MS hardware and software to advance top-down MS closer to the mainstream, top-down proteomics still faces major challenges. In particular, the proteome is extremely complex and has a high dynamic range in addition to the low solubility of many proteins, making it highly challenging for high-throughput proteomic study. Building on the success in the last funding period, in this multiple-PI renewal application, we will continue to develop innovative strategies empowered by nanotechnology and materials/organic chemistry to further address the challenges in top-down proteomics. The specific objectives of this proposal are: 1) To address the protein solubility challenge, we will develop a novel strategy enabled by a photocleavable surfactant for extracellular matrix (ECM) proteomics; and design, synthesize, and evaluate a novel class of photocleavable nonionic surfactants that can retain the native structures of proteins for native MS-based top-down proteomics. 2) To address the high dynamic range challenge, we will develop novel surface functionalized magnetic nanoparticles (NPs) to mimic antibodies for capturing and enriching low abundance proteins, such as cardiac troponin I (cTnI, a gold-standard biomarker for heart diseases) from tissues/blood and G-protein coupled receptors (GPCRs, a major class of drug targets) from cells/tissues, for downstream comprehensive analysis of all proteoforms by top-down proteomics. Our highly interdisciplinary approach integrates materials chemistry/nanotechnology with top-down MS-based proteomics, and is based on an existing productive collaboration between two PIs that has led to significant progress and publications from the past funding period. Success in our proposed research will provide innovative tools to enable top-down proteomics of poorly soluble and low abundance proteins, which will lay important technological foundation for understanding the critical role that ECM plays in disease progression in cancer and cardiac diseases, defining the structure-function relationship of native membrane complexes, developing a comprehensive cTnI assay for the diagnosis of cardiac diseases with high accuracy, and understanding the important roles of GPCR signaling during the onset of numerous human diseases including cancer, diabetes, and cardiovascular diseases.
总结

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Comprehensive characterization of monoclonal antibody by Fourier transform ion cyclotron resonance mass spectrometry.
  • DOI:
    10.1080/19420862.2018.1525253
  • 发表时间:
    2019-01
  • 期刊:
  • 影响因子:
    5.3
  • 作者:
    Jin Y;Lin Z;Xu Q;Fu C;Zhang Z;Zhang Q;Pritts WA;Ge Y
  • 通讯作者:
    Ge Y
PP2A-B' holoenzyme substrate recognition, regulation and role in cytokinesis.
  • DOI:
    10.1038/celldisc.2017.27
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    33.5
  • 作者:
    Wu CG;Chen H;Guo F;Yadav VK;Mcilwain SJ;Rowse M;Choudhary A;Lin Z;Li Y;Gu T;Zheng A;Xu Q;Lee W;Resch E;Johnson B;Day J;Ge Y;Ong IM;Burkard ME;Ivarsson Y;Xing Y
  • 通讯作者:
    Xing Y
A Family of Photolabile Nitroveratryl-Based Surfactants That Self-Assemble into Photodegradable Supramolecular Structures.
Electrophilic probes for deciphering substrate recognition by O-GlcNAc transferase.
  • DOI:
    10.1038/nchembio.2494
  • 发表时间:
    2017-12
  • 期刊:
  • 影响因子:
    14.8
  • 作者:
    Hu CW;Worth M;Fan D;Li B;Li H;Lu L;Zhong X;Lin Z;Wei L;Ge Y;Li L;Jiang J
  • 通讯作者:
    Jiang J
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Ying Ge其他文献

Ying Ge的其他文献

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{{ truncateString('Ying Ge', 18)}}的其他基金

MASH Explorer, a Comprehensive Software Environment for Top-Down Proteomics
MASH Explorer,自上而下蛋白质组学的综合软件环境
  • 批准号:
    9904714
  • 财政年份:
    2018
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    10727013
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    9982021
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Materials Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    10653557
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    10669640
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    10246801
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    10437916
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    9010161
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Enabling Top-Down Proteomics through Material Chemistry and Nanotechnology
通过材料化学和纳米技术实现自上而下的蛋白质组学
  • 批准号:
    9336949
  • 财政年份:
    2015
  • 资助金额:
    $ 19.99万
  • 项目类别:
Deciphering Myofilament Modifications in Ischemic Cardiomyopathy
破译缺血性心肌病中的肌丝修饰
  • 批准号:
    8605545
  • 财政年份:
    2013
  • 资助金额:
    $ 19.99万
  • 项目类别:

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University of Aberdeen and Vertebrate Antibodies Limited KTP 23_24 R1
阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
  • 批准号:
    10073243
  • 财政年份:
    2024
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    $ 19.99万
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Role of Natural Antibodies and B1 cells in Fibroproliferative Lung Disease
天然抗体和 B1 细胞在纤维增生性肺病中的作用
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    10752129
  • 财政年份:
    2024
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    $ 19.99万
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CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
  • 批准号:
    2339201
  • 财政年份:
    2024
  • 资助金额:
    $ 19.99万
  • 项目类别:
    Continuing Grant
Isolation and characterisation of monoclonal antibodies for the treatment or prevention of antibiotic resistant Acinetobacter baumannii infections
用于治疗或预防抗生素耐药鲍曼不动杆菌感染的单克隆抗体的分离和表征
  • 批准号:
    MR/Y008693/1
  • 财政年份:
    2024
  • 资助金额:
    $ 19.99万
  • 项目类别:
    Research Grant
Developing first-in-class aggregation-specific antibodies for a severe genetic neurological disease
开发针对严重遗传神经系统疾病的一流聚集特异性抗体
  • 批准号:
    10076445
  • 财政年份:
    2023
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    $ 19.99万
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发现中枢神经系统脱髓鞘疾病中的新型节点抗体并通过视神经脱髓鞘模型阐明其机制
  • 批准号:
    23K14783
  • 财政年份:
    2023
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  • 批准号:
    23KJ0394
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抗体在戊型肝炎病毒感染中的作用
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    10639161
  • 财政年份:
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    $ 19.99万
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Defining the protective or pathologic role of antibodies in Post-Ebola Syndrome
定义抗体在埃博拉后综合症中的保护或病理作用
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    10752441
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    2023
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Human CMV monoclonal antibodies as therapeutics to inhibit virus infection and dissemination
人 CMV 单克隆抗体作为抑制病毒感染和传播的治疗药物
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    10867639
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