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Quadruple-resonance HFXY 1.3 mm CP-MAS probe for a solid-state NMR wide-bore magnet

用于固态 NMR 大口径磁体的四共振 HFXY 1.3 mm CP-MAS 探头

基本信息

批准号：
10798817
负责人：
Mei Hong
金额：
$ 19.75万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2025-06-30
项目状态：
未结题

项目摘要

Project Summary This proposal aims to elucidate the structures and mechanism of action of three ion channels and transporters of viruses and bacteria: the SARS-CoV-2 envelope (E) protein, the influenza M2 protein, and the multidrug-resistant bacterial transporter, EmrE. All three proteins form membrane-bound ion channels or transporters that are important for the lifecycle and function of the respective virus or bacteria. Molecular structural information about these membrane proteins forms the basis for designing antiviral and antibiotic compounds to prevent viral and bacterial infections. For all three membrane proteins, fluorine has been strategically incorporated into the proteins or the substrate to enable the measurement of nanometer distances by solid-state NMR. These distances are powerful restraints for the interhelical assembly of these membrane proteins and the protein-substrate binding-site structure. However, the current NMR probe in the Hong lab for these 19F-based NMR experiments is limited by the spinning speed and the availability of only one radiofrequency channel beside 1H and 19F. To obtain higher-resolution NMR spectra and to correlate 1H and 19F signals with multiple nuclei, we request an 1.3 mm HFXY probe that can spin samples to 65 kHz and that tunes to two heteronuclear channels (X and Y). Using this 1.3 mm HFXY probe, we will investigate the open-state structure of the SARS-CoV-2 E protein, the open-state structure of an influenza BM2 mutant, and the substrate-binding site geometry of the EmrE protein.

项目摘要本文旨在阐明三种离子的结构和作用机理病毒和细菌的通道和转运蛋白：SARS-CoV-2包膜（E）蛋白，流感M2蛋白和多药耐药细菌转运蛋白EmrE。所有三种蛋白质形成对生命周期很重要的膜结合离子通道或转运蛋白，病毒或细菌各自的功能。分子结构信息膜蛋白构成了设计抗病毒和抗生素化合物的基础，病毒和细菌感染。对于所有三种膜蛋白，氟一直是战略性的结合到蛋白质或基质中以实现纳米距离的测量固态核磁共振这些距离是这些分子的螺旋间组装的有力限制。膜蛋白和蛋白质-底物结合位点结构。然而，目前的NMR Hong实验室中用于这些基于19 F的NMR实验的探针受到旋转速度的限制，除了1H和19 F之外，只有一个射频通道可用。为了获得更高的分辨率 NMR光谱，并将1H和19 F信号与多个核相关联，我们要求1.3 mm HFXY 该探头可以将样本旋转到65 kHz，并调谐到两个异频通道（X和Y）。使用这种1.3 mm HFXY探针，我们将研究SARS-CoV-2的开放状态结构 E蛋白，流感BM 2突变体的开放状态结构和底物结合位点 EmrE蛋白的几何结构。