Human Tissue Core

人体组织核心

• 批准号: 10806550
• 负责人: Wenqin Luo
• 金额: $ 33.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-20 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10806550
• 关键词: Affect; Afferent Neurons; Brain; Cells; Collaborations; Collection; Communication; Communities; Complex; Cryoultramicrotomy; Data; Databases; Development; Disease; Ensure; Environment; Epigenetic Process; Evaluation; Facial Pain; Foundations; Genes; Goals; Head; Headache Disorders; Health; Helping to End Addiction Long-term; Household; Human; Institutional Review Boards; International; Knowledge; Letters; Location; Measures; Mediating; Medicine; Migraine; Molecular; National Institute of Mental Health; Neurons; Neurosurgeon; Pain; Pain Clinics; Pain Research; Patients; Physiological; Postdoctoral Fellow; Procedures; Protocols documentation; Quality Control; RNA; Radiation; Research; Research Personnel; Research Project Grants; Research Support; Resources; Safety; Sampling; Site; Spinal Ganglia; Structure of trigeminal ganglion; Tissue Procurements; Tissue Sample; Tissues; Training; Trigeminal System; United States National Institutes of Health; Work; allodynia; chronic painful condition; effective therapy; experience; experimental study; human RNA sequencing; human tissue; migraine treatment; multidisciplinary; nervous system disorder; neuronal cell body; neurosurgery; novel; response; single-cell RNA sequencing; somatosensory; tissue archive; tissue processing; transcriptome sequencing

Migraine, one of the most common primary headache disorders, affects 1 in 4 US households. This complex neurologic disorder is mediated in part by alterations in trigeminal somatosensation, which manifests as head/fa- cial pain and/or trigeminal allodynia. Effective treatments for migraine are still limited, and our knowledge about human trigeminal system at baseline and migraine conditions are sparse. In response to RFA-NS-22-018, HEAL Initiative: Discovery and Functional Evaluation of Human Pain-associated Genes & Cells, we propose to form the Penn Human Precision Pain Center (Penn HPPC) to elucidate molecular, cellular, epigenetic, and physiological profiles of human trigeminal ganglion (TG) sensory neurons at baseline and migraine conditions. The Penn HPPC will be composed of Penn and international investigators with multidisciplinary expertise. The PI, two MPIs, and two co-Is are currently collaborating on a single-soma deep RNA-seq of human dorsal root ganglion (DRG) project, which form a strong foundation for this application. The Penn HPPC will contain three cores and perform three projects. Among them, the human tissue core will function as the sole entity for procuring and storing human tissues and distributing human tissues among research projects. The Penn HPPC human tissue procurement and processing core will also coordinate with other U19 centers and the NIH HEAL Initiative PRECISION Human Pain network for human tissue procurement, sharing, and SOP (Standard Operat- ing Procedure) development. The human tissue core will be led by the PI, Dr. Luo, and supported by her research associate, Dr. Yu, and a coordinator. The human tissue core will also include two UPenn neurosurgeons, Drs Ali Zarina and Eric Zager, and a coordinator in the department of neurosurgery. Specifically, the human tissue core will coordinate with different human tissue procurement sites, including the human tissue cores of other U19 centers, for procuring human trigeminal ganglion (TGs) and dorsal root ganglion (DRG) tissues, perform quality control of all procured tissues, stock and archive the tissues, distribute tissues among the three research pro- jects, ensure compliances and trainings related to human tissues, and work with the administrative core to dis- seminate optimized protocols and procedures related to human TGs and DRGs to the HEAL community and the pain research field. Together with other components of the Penn HPPC, the human tissue procurement and processing core will help to achieve the proposed goals of Penn HPPC as well as the overarching goal of the NIH HEAL Initiative PRECISION Human Pain network.

偏头痛是最常见的原发性头痛疾病之一，每四个美国家庭中就有一个受到影响。这个建筑群 神经功能障碍的部分原因是三叉神经躯体感觉的改变，表现为头/脸-头-面-头-面-头-头-面-面 三叉神经痛和/或三叉神经痛。偏头痛的有效治疗方法仍然有限，我们对 人类三叉神经系统在基线和偏头痛的情况下是稀疏的。响应RFA-NS-22-018，修复 倡议：人类疼痛相关基因和细胞的发现和功能评估，我们建议 成立宾夕法尼亚人类精确疼痛中心(Penn HPPC)，以阐明分子、细胞、表观遗传学和 人三叉神经节(TG)感觉神经元在基线和偏头痛状态下的生理特征。 宾夕法尼亚大学HPPC将由宾夕法尼亚大学和具有多学科专业知识的国际调查人员组成。这个 PI、两个MPI和两个协同IS目前正在合作研究人类背根的单胞体深度RNA序列 Ganglion(DRG)项目，这为这一应用程序奠定了坚实的基础。宾夕法尼亚大学的HPPC将包含三个 核心，并执行三个项目。其中，人体组织核心将作为唯一实体发挥作用 获取和储存人体组织，并在研究项目中分发人体组织。宾夕法尼亚大学的HPPC 人体组织采购和处理核心还将与其他U19中心和NIH Hear协调 用于人体组织采购、共享和SOP(标准操作)的主动精密人类疼痛网络 ING程序)开发。人体组织核心将由PI罗博士领导，并由她的研究提供支持 助理、余博士和一名协调员。人体组织核心还将包括两名宾夕法尼亚大学神经外科医生阿里博士 扎里娜和埃里克·扎格，神经外科的协调员。具体地说，人体组织核心 将与不同的人体组织采购地点进行协调，包括其他U19的人体组织核心 用于获取人三叉神经节(TGS)和背根神经节(DRG)组织的中心 控制所有采购的组织，储存和存档组织，在三个研究项目之间分配组织 目标，确保与人体组织有关的合规和培训，并与行政核心合作，分发 将与人类TGS和DRGs相关的优化方案和程序传授给Hear社区和 疼痛研究领域。与宾夕法尼亚大学HPPC的其他组成部分一起，人体组织采购和 处理核心将有助于实现宾夕法尼亚大学氟氯烃淘汰计划的拟议目标以及 美国国立卫生研究院医治计划精确人类疼痛网络。