Spatial genetics investigation of multinucleated cells -Administrative Supplement - Equipment

点击翻译按钮获取中文摘要

Project Summary/Abstract The PI holds a current NIGMS R35 research award 1R35GM147209 (Spatial genetics investigation of multinucleated cells). Under this award, the PI is requesting supplemental equipment funds that will be used to purchase MERSCOPE, the first and only commercial platform for Multiplexed Error-Robust Fluorescence in situ Hybridization (MERFISH) technology, which allows the simultaneous single-molecule imaging of hundreds of mRNA species in hundreds of thousands of cells. The MERSCOPE Platform also offers a suite of software tools and optimized reagents that streamline the whole experiment process from sample to high-quality spatial transcriptome data. It thus reduces human interventions and procedural errors. The MERSCOPE system will serve as the workhorse for the parent R35 project. The main goal of the funded R35 grant is to perform in-depth spatial investigations of the basic genetic mechanism operated in gigantic syncytial cells like myofibers. These unique cellular systems contain hundreds of nuclei and currently little is known about the interplay and coordination of gene expression programs in heavily multinucleated cells. This administrative supplement, if granted, will facilitate the purchase of a MERSCOPE Platform, which will serve a critical role in the success of the funded R35 project. The Institutional supports were acquired with funds ready to purchase service contracts throughout the life of the parent R35 project.