Spatial genetics investigation of multinucleated cells -Administrative Supplement - Equipment

多核细胞空间遗传学研究-行政补遗-设备

• 批准号: 10799074
• 负责人: Pengpeng Bi
• 金额: $ 25万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799074
• 关键词: Administrative Supplement; Award; Biological; Cell Nucleus; Cells; Complex; Contract Services; Data; Detection; Dimensions; Equipment; Fluorescent in Situ Hybridization; Funding; Gene Expression; Genetic; Goals; Grant; Human; Institution; Intervention; Investigation; Life; Measurement; Messenger RNA; National Institute of General Medical Sciences; Parents; Process; Reagent; Research; Role; Sampling; Software Tools; System; Technology; Work; experimental study; instrument; manufacture; molecular imaging; programs; single molecule; success; tool; transcriptome

Project Summary/Abstract The PI holds a current NIGMS R35 research award 1R35GM147209 (Spatial genetics investigation of multinucleated cells). Under this award, the PI is requesting supplemental equipment funds that will be used to purchase MERSCOPE, the first and only commercial platform for Multiplexed Error-Robust Fluorescence in situ Hybridization (MERFISH) technology, which allows the simultaneous single-molecule imaging of hundreds of mRNA species in hundreds of thousands of cells. The MERSCOPE Platform also offers a suite of software tools and optimized reagents that streamline the whole experiment process from sample to high-quality spatial transcriptome data. It thus reduces human interventions and procedural errors. The MERSCOPE system will serve as the workhorse for the parent R35 project. The main goal of the funded R35 grant is to perform in-depth spatial investigations of the basic genetic mechanism operated in gigantic syncytial cells like myofibers. These unique cellular systems contain hundreds of nuclei and currently little is known about the interplay and coordination of gene expression programs in heavily multinucleated cells. This administrative supplement, if granted, will facilitate the purchase of a MERSCOPE Platform, which will serve a critical role in the success of the funded R35 project. The Institutional supports were acquired with funds ready to purchase service contracts throughout the life of the parent R35 project.

项目总结/摘要 PI持有当前NIGMS R35研究奖1R35GM147209（空间遗传学 多核细胞的研究）。根据这项裁决，PI要求补充 设备资金将用于购买MERSCOPE，第一个也是唯一的商业 多重误差稳健荧光原位杂交（MERFISH）技术平台， 它允许同时对数百种mRNA进行单分子成像， 成千上万的细胞。MERSCOPE平台还提供一套软件工具 优化试剂，简化从样品到高质量的整个实验过程 空间转录组数据。因此，它减少了人为干预和程序错误。的 MERSCOPE系统将作为R35项目的主力。的主要目标 R35基金将对基本遗传机制进行深入的空间研究 在巨大的合胞体细胞中运作，就像肌纤维一样。这些独特的细胞系统包含 数百个细胞核，目前对基因的相互作用和协调知之甚少 在多核细胞中的表达程序。这项行政补助，如果批准， 将促进购买MERSCOPE平台，这将在成功中发挥关键作用 关于R35项目机构支持是用准备购买的资金获得的 在R35项目的整个生命周期内，