Spatial genetics investigation of multinucleated cells

多核细胞的空间遗传学研究

• 批准号: 10500990
• 负责人: Pengpeng Bi
• 金额: $ 37.75万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10500990
• 关键词: Animals; Award; Behavior; Behavior Control; Biochemical; Biological; Cardiac Myocytes; Cell Communication; Cell Nucleus; Cells; Cellularity; Communities; Development; Environment; Gene Expression; Genetic; Genomics; Giant Cells; Homeostasis; Human; Imaging technology; Investigation; Knowledge; Messenger RNA; Methodology; Modeling; Mononuclear; Muscle; Nature; Polyploidy; Research; Retinal blind spot; Reverse engineering; Stimulus; Syncytiotrophoblast; System; Techniques; Technology; Tissues; body system; cancer cell; cell type; cellular engineering; fascinate; fitness; gene function; human disease; human tissue; in vivo; innovation; intercellular communication; programs; response; single molecule; trafficking; transcriptomics

Project Summary/Abstract Organ systems are composed of a wealth of cellular subpopulations whose spatial organization within a given tissue are deeply intertwined with their functions and homeostasis controls. Similarly, within the space of a single-cell, the biochemical environment is also heterogeneous where the dynamic interactions of molecules determine the fitness, behavior and fate of the cell. Our understanding of the cell interactions and the biomolecule interactions has recently been transformed by the revolutionary single-cell genomics and single-molecule imaging technologies. However, the development and application of these technologies have been exclusively centered on the context of mononuclear cellularity. Hiding in the blind spot are multinucleated cell-types, including myofibers, cardiomyocytes, syncytiotrophoblasts, certain cancer cells, all of which cause devasting human disease when go awry. The syncytial nature of the multinucleated cells, which possess the polyploidy and often the vast cytosolic volumes, raises fascinating questions with respect to the spatial organizations of the cell-cell interactions and biomolecule distributions. The fundamental yet largely unknown questions include: 1) How heterogeneous is the tissue microenvironment that surrounds the syncytial cells? 2) Do nuclei from the shared cell body coordinate gene expression in response to the external stimuli and cell-cell communications? 3) What is the mechanism that governs the transports and localizations of mRNAs in the multinucleated cells? Our research program exploits the unique features of specialized cell-types as a means to understand mechanistic underpinnings of various developmental systems. This proposal leverages myofiber as the uniquely-suited model to investigate the above questions in a spatially defined manner. First, we will develop and deploy new methodologies to probe the spatial transcriptomics for the multiple types of syncytial tissues. Second, we will devise the reverse engineering strategy to assemble the single-syncytium of human muscle with genetic trackabilities and later graft them to live animals for the in vivo study of the cell-cell communication and intra-syncytium mRNA distributions. Third, we will conduct the in-depth gene function and mechanism studies to unveil new paradigms of intercellular communication and the intracellular mRNA trafficking. Broadly, this research program relies on our diverse expertise in genomics, cell engineering and computation such that we can create a virtuous cycle of innovation and discovery over the course of the MIRA award. We anticipate that the knowledge and techniques will benefit the greater biological community, including genetics, cell biologists and developmental biologists.

项目总结/文摘