Admin Supplement: The molecular mechanisms and functional significance of gene positioning

管理补充：基因定位的分子机制和功能意义

• 批准号: 10806730
• 负责人: Jason Hays Brickner
• 金额: $ 9.42万
• 依托单位: NORTHWESTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10806730
• 关键词: Aging; Animals; Biological Models; Cell Nucleus; Cells; Chromatin Structure; Chromosomes; Complex; Environment; Future; Gene Expression; Gene Order; Genes; Genetic Transcription; Genome; Goals; Human; Life Style; Mediating; Molecular; Nuclear Pore Complex; Physiology; Saccharomycetales; Signal Transduction; Transcriptional Regulation; Work; gene interaction; response; transcription factor; yeast genome

Project Summary Understanding the molecular control of transcriptional responses of cells to environmental signals is essential to understand how environment, aging and lifestyle impact physiology. My lab has discovered that the position of genes is actively controlled by cells and that this impacts transcription in several ways. Although we study this phenomenon in the simple budding yeast, it is conserved to more complex animals, including humans. We recently showed that a majority of transcription factors control the interaction of genes with the Nuclear Pore Complex (NPC), impacting the spatial arrangement of genes and the interaction of chromosomes. Furthermore, we have shown that this interaction with the NPC can either promote stronger transcription and alter chromatin structure to poise genes for future expression. Thus, transcription factor-mediated targeting to the NPC likely has broad effects on both the spatial organization of the genome and gene expression. The goals of this work are to determine the molecular mechanisms by which transcription factors and NPCs spatially arrange the yeast genome and to understand how these interactions impact gene expression and chromatin structure.

项目总结

项目成果

The Nuclear Pore Complex as a Transcription Regulator.

• DOI: 10.1101/cshperspect.a039438
• 发表时间: 2021-06
• 期刊: Cold Spring Harbor perspectives in biology
• 影响因子: 7.2
• 作者: M. Sumner;Jason H. Brickner

Mitotically heritable, RNA polymerase II-independent H3K4 dimethylation stimulates INO1 transcriptional memory.

有丝分裂性的RNA聚合酶II独立的H3K4二甲基化刺激INO1转录记忆。

• DOI: 10.7554/elife.77646
• 发表时间: 2022-05-17
• 期刊: ELIFE
• 影响因子: 7.7
• 作者: Sump, Bethany;Brickner, Donna G.;D'Urso, Agustina;Kim, Seo Hyun;Brickner, Jason H.
• 通讯作者: Brickner, Jason H.

ChEC-seq2: an improved Chromatin Endogenous Cleavage sequencing method and bioinformatic analysis pipeline for mapping in vivo protein-DNA interactions.

ChEC-seq2：一种改进的染色质内源切割测序方法和生物信息学分析流程，用于绘制体内蛋白质-DNA 相互作用图谱。

• DOI: 10.1101/2023.10.15.562421
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: VanBelzen,Jake;Duan,Chengzhe;Brickner,DonnaGarvey;Brickner,JasonH
• 通讯作者: Brickner,JasonH