Signaling Mechanisms and Cellular Functions of a Ciliopathy-associated Protein Kinase

纤毛病相关蛋白激酶的信号传导机制和细胞功能

• 批准号: 10799202
• 负责人: Zheng Fu
• 金额: $ 3.13万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799202
• 关键词: Apical; Cell physiology; Chemicals; Cilia; Cues; DNA Sequence Alteration; Defect; Disease; Esthesia; Film; Fluorescence; Functional disorder; Genes; Human; Knowledge; Mammalian Cell; Methods; Microtubules; Molecular; Mutation; Organelles; Pathogenicity; Phosphotransferases; Play; Protein Kinase; Research; Research Project Grants; Role; Signal Transduction; Structure; Surface; Western Blotting; base; ciliopathy; cilium biogenesis; human disease; imaging system; response; success

Abstract Ciliopathies comprise an expanding group of human disorders associated with genetic mutations causing cilia dysfunction. The primary cilium is a microtubule (MT)-based organelle that protrudes from the apical surface of nearly every mammalian cell and plays a critical role in chemical sensation, signal transduction, and control of various cellular functions. To date, there are still major gaps in our knowledge about the dynamic structure and function of the primary cilium, and the underlying molecular basis of ciliopathies. Our research project is focused on elucidating the molecular mechanism by which pathogenic mutations in the human CILK1 (ciliogenesis associated kinase 1) gene cause ciliopathies. Our research will exert strong impact on basic knowledge about the primary cilium and significantly advance our understanding of the disease mechanisms underlying human ciliopathies. Western blotting is the most frequently used method in our experimentation and the quality of Western blots is essential for the success of this project. In this supplement, we request the purchase of Amersham ImageQuant™ 800 Western blot imaging system to replace an outdated film processor in order to meet our need to achieve high sensitivity and accurate quantification for both chemiluminescence and multi-channel fluorescence signals on Western blots.

摘要