DROSOPHILA GENOMICS RESOURCE CENTER

项目摘要： 果蝇基因组学资源中心（DGRC）支持国际 利用果蝇进行生物医学研究的科学家社区。的 DGRC的使命是：1）通过获取， 归档、管理和分发基因组学资源，包括克隆、载体和 细胞系; 2）通过提供指导，促进这些基因组学资源的有效利用 和支持; 3）改善基因组学资源和协议， 果蝇研究通过保存重要的研究材料并分发给 有效地，DGRC确保经济的访问和提高科学的严谨性， 再现性这项建议的第一个目的是继续和加强成功的 DGRC计划用于获取，分发基因组学资源并促进其 有效利用。这将包括扩大和更新数据管理系统， 网络界面和用户支持，以及继续有效回收成本 程序.其目标是最大限度地提高DGRC的长期可行性， 用户和NIH。第二个目的是增加果蝇作为模型的实用性 系统通过四个项目产生新的资源：1）调查转染 和跨modENCODE细胞系的基因标记的CRISPR/Cas9效率，2） 生成通用的基于CRISPR/Cas9的转染工具包，用于插入 构建体和随后在任何果蝇中产生稳定转化体的能力 细胞系，3）建立神经母细胞细胞系作为干细胞生物学的模型，和4） 创建新的代谢传感器用于表征细胞系中的生理过程 和果蝇组织。

Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The second aim is to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.