DROSOPHILA GENOMICS RESOURCE CENTER

项目摘要： 果蝇基因组学资源中心（DGRC）支持国际社会， 科学家利用果蝇进行生物医学研究。联合国的使命 DGRC将1）通过获取、存档、管理， 和分发基因组学资源，包括克隆、载体和细胞系; 2）促进 通过提供指导和支持，有效利用这些基因组学资源;以及3） 改善果蝇研究的基因组学资源和方案。通过 保护重要的研究材料并有效地分发，DGRC保证 经济的访问和提高科学的严谨性和可重复性。第一个目标是 建议是继续和加强成功的DGRC计划， 分配基因组学资源并促进其有效利用。这将包括 扩充和更新数据管理系统、网络界面和用户支持， 并继续实施有效的成本回收计划。我们的目标是最大限度地实现长期 DGRC的可行性及其对用户和NIH的好处。第二个目标是增加 通过四个项目产生新资源，利用果蝇作为模型系统：1） 调查基因标记的转染和CRISPR/Cas9效率， modENCODE细胞系，2）产生基于通用CRISPR/Cas9的转染工具包 对于构建体的插入和随后在大肠杆菌中产生稳定转化体的能力， 任何果蝇细胞系，3）建立神经母细胞系作为干细胞模型， 生物学，以及4）创建新的代谢传感器，用于表征生物学中的生理过程。 细胞系和果蝇组织。

Project Summary: The Drosophila Genomics Resource Center (DGRC) supports the international community of scientists utilizing Drosophila melanogaster for biomedical research. The mission of the DGRC is to 1) provide broad access to genomics resources by acquiring, archiving, curating, and distributing genomics resources including clones, vectors, and cell lines; 2) facilitate effective use of these genomics resources by providing guidance and support; and 3) improve the genomics resources and protocols available for Drosophila research. By preserving vital research materials and distributing them efficiently, the DGRC assures economical access and enhances scientific rigor and reproducibility. The first aim of this proposal is to continue and strengthen the successful DGRC programs for acquiring, distributing genomics resources and facilitating their effective use. This will include augmenting and updating data management systems, the web interface and user support, as well as continuing the effective cost recovery program. The goal is to maximize the long-term viability of the DGRC and its benefits to users and the NIH. The second aim is to increase the utility of Drosophila as a model system by generating new resources through four projects: 1) surveying the transfection and CRISPR/Cas9 efficiency of gene tagging across modENCODE cell lines, 2) generating a universal CRISPR/Cas9 based transfection toolkit for the insertion of constructs and the subsequent ability to generate stable transformants in any Drosophila cell line, 3) establishing a neuroblast cell line as a model for stem cell biology, and 4) creating new metabolic sensors for characterizing physiological processes in cell lines and Drosophila tissues.