A multi-laboratory validation of the extraction of fat-soluble vitamins from tissue and feed and their analysis by LC-MS/MS

从组织和饲料中提取脂溶性维生素及其 LC-MS/MS 分析的多实验室验证

• 批准号: 10829100
• 负责人: John Philip Buchweitz
• 金额: $ 5.79万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10829100
• 关键词: multi; laboratory; validation; extraction; fat

PAR-18-604 Vet-LIRN Network Capacity-Building Project A multi-laboratory validation of the extraction of fat-soluble vitamins from tissue and feed and their analysis by LC-MS/MS Abstract The fat-soluble vitamins A, E, and D are essential dietary components for all animal species. Although required in small quantities, these compounds exert a multitude of metabolic and physiologic effects through all stages of an animal’s life. Because these vitamins or their precursors are naturally occurring, most free ranging animals can maintain adequate levels through the browse or forage they consume. However, processed grains and other ingredients used in the generation of commercial feeds for companion, production, and specialty species are challenged with the propensity of vitamins toward degradation. Accordingly, the commercial feed industry supplements diets with synthetic forms of these vitamins to provide adequate nutrient value. However, storage conditions and mixing errors can adversely impact the final concentration that is consumed. As a result, nutrient vitamin concentrations may be either inadequate and lead to clinical signs of deficiency or excessive and exhibit manifestations of toxicosis. Clinically, both deficiencies and toxicoses can be established ante-mortem through the measurement of serum vitamin A, E, or the vitamin D metabolite, 25-hydroxy vitamin D (25-OH D). For the post-mortem animal, tissue vitamin analysis is generally performed with liver being the sample of choice for vitamins A and E, and kidney being the best sample for 25-OH D. A clinical diagnosis that is suggestive of vitamin deficiency or excess generally (but not always) implicates a potential problem with feed. Current methods to address fat soluble vitamin content in feed are somewhat onerous, time-consuming, and may utilize outdated equipment. We propose a method of extraction and analysis for both tissues and feed that is simple, accurate, and reproducible across laboratories within the CVM Vet-LIRN community, Michigan State University Veterinary Diagnostic Laboratory (MSU VDL), Kansas State University Veterinary Diagnostic Laboratory (KSU VDL), Tennessee Department of Agriculture Kord Animal Health Diagnostic Laboratory (TN Ag Lab), Texas A&M University Veterinary Medical Diagnostic Laboratory (TVMDL), University of California Davis California Animal Health and Food Safety (CAHFS) lab, and University of Guelph Animal Health Laboratory (Guelph AHL) equipped with liquid chromatography tandem mass spectrometry.

PAR-18-604 Vet-LIRN 网络能力建设项目 从组织和饲料中提取脂溶性维生素及其效果的多实验室验证 LC-MS/MS 分析 抽象的 脂溶性维生素 A、E 和 D 是所有动物物种必需的膳食成分。虽然需要 这些化合物只需少量即可在各个阶段发挥多种代谢和生理作用 动物的生命。因为这些维生素或其前体是天然存在的，大多数是自由放养的 动物可以通过它们消耗的食物或饲料来维持足够的水平。然而，经过加工的谷物 以及用于生产伴侣饲料、生产饲料和特种饲料的其他成分 物种面临着维生素降解倾向的挑战。因此，商业饲料 工业界用这些维生素的合成形式补充饮食，以提供足够的营养价值。然而， 储存条件和混合错误会对消耗的最终浓度产生不利影响。作为一个 结果，营养维生素浓度可能不足并导致缺乏或缺乏的临床症状 过量并表现出中毒症状。临床上可以确定缺乏症和中毒症 宰前通过测量血清维生素 A、E 或维生素 D 代谢物 25-羟基维生素 D(25-OH D)。对于死后的动物，组织维生素分析通常以肝脏为分析对象。 维生素 A 和 E 的首选样本，肾脏是 25-OH D 的最佳样本。临床诊断表明 暗示维生素缺乏或过量，通常（但并非总是）暗示存在潜在问题 喂养。目前解决饲料中脂溶性维生素含量的方法有些繁琐、耗时、 并可能使用过时的设备。我们提出了一种组织和饲料的提取和分析方法 密歇根州 CVM Vet-LIRN 社区内的实验室简单、准确且可重复 堪萨斯州立大学兽医诊断实验室 (MSU VDL) 田纳西州农业部实验室 (KSU VDL) Kord 动物健康诊断实验室 (TN Ag Lab），德克萨斯农工大学兽医医学诊断实验室（TVMDL），加州大学 戴维斯加州动物健康和食品安全 (CAHFS) 实验室和圭尔夫大学动物健康 实验室（Guelph AHL）配备液相色谱串联质谱仪。