Increasing SARS-CoV-2 (COVID-19) testing capacity at the Nebraska Veterinary Diagnostic Center

项目总结/摘要 内布拉斯加州兽医诊断中心（NVDC）是一家提供全方位服务的兽医诊断中心。 实验室，完全由美国兽医实验室协会认可 诊断师，是FDA-CVM Vet-LIRN网络的成员，是国家动物卫生 实验室网络（NAHLN）1级实验室，是国家实验室认证实验室 家禽改良计划测试。该实验室每年处理超过30万份标本 来自各种物种，包括牲畜、伴侣动物、家禽、野生动物和动物园 动物作为加强动物SARS-CoV-2监测的合作努力的一部分， 2022-2023年，超过3，600只不同动物物种接受了测试。增加 NVDC正在寻求在动物中进行SARS-CoV-2诊断测试的能力和能力， 购买数字PCR系统。该设备将使NVDC能够增强 SARS-CoV-2诊断测试，允许极其灵敏地检测SARS-CoV-2， 动物标本此外，数字PCR方法允许定量SAR- 临床样本中的CoV-2基因组，这将有助于告知临床医生和One Health合作伙伴， 评估病毒水平和在动物中的传播。

Project Summary/Abstract The Nebraska Veterinary Diagnostic Center (NVDC) is a full-service veterinary diagnostic laboratory, is fully accredited by the American Association of Veterinary Laboratory Diagnosticians, is a member of FDA-CVM Vet-LIRN network, is a National Animal Health Laboratory Network (NAHLN) Level 1 laboratory, and is a certificated laboratory for National Poultry Improvement Plan testing. The laboratory processes over 300,000 specimens annually from a variety of species including livestock, companion animals, poultry, wildlife, and zoo animals. As part of a collaborative effort to enhance surveillance for SARS-CoV-2 in animals, more than 3,600 animals diverse animal species were tested in 2022-2023. To increase the diagnostics testing capacity and capabilities for SARS-CoV-2 in animals, the NVDC is seeking funds to purchase a digital PCR system. This equipment will enable the NVDC to enhance SARS-CoV-2 diagnostic testing by allowing extremely sensitive detection of SARS-CoV-2 in animal specimens. Additionally, the digital PCR approaches allows for quantification of SAR- CoV-2 genomes in clinical samples, which will help inform clinicians and One Health partners in assessing virus levels and transmission in animals.