Visium spatial transcriptome analysis add on to the current proposed scRNA seq analysis

摘要： 本申请是为了响应特别利益通知（NOSI）而提交的， 编号为NOT-CA-23-045。 顺铂诱导的急性肾损伤（C-AKI）是一种复杂的疾病， mortality.我们的研究团队旨在研究所涉及的细胞和分子机制 阿基和治疗反应。我们已经使用单细胞RNA测序（scRNA seq）， 研究单个细胞中的基因表达水平。然而，scRNA seq缺乏空间背景， 这对于理解细胞间的相互作用和微环境很重要。因此，我们认为， 我们建议增加Visium空间转录组学，这是一种尖端技术， 组织成像与RNA测序，我们的研究。我们的目标是分析基因表达 以空间分辨的方式检测细胞水平，识别新的细胞类型及其空间分布，以及 生成肾脏组织中基因表达模式的高分辨率图。探讨 参与免疫反应和其他过程的基因的空间表达模式 受APE 2-阿索治疗的影响，我们提出了一项使用Visium空间转录组学的研究， APE 2 ASO处理的顺铂诱导的阿基模型。通过将Visium空间转录组学添加到 我们正在进行阿基研究，旨在更深入地了解细胞和分子 并确定潜在的治疗靶点。

ABSTRACT: This application is being submitted in response to the Notice of Special Interest (NOSI) identified as NOT-CA-23-045. Cisplatin induced Acute kidney injury (C-AKI) is a complex condition with significant morbidity and mortality. Our research team aims to investigate the cellular and molecular mechanisms involved in AKI and therapy response. We have used single-cell RNA sequencing (scRNA seq) to investigate gene expression levels in individual cells. However, scRNA seq lacks spatial context, which is important for understanding cell-cell interactions and the microenvironment. Therefore, we propose adding Visium Spatial Transcriptomics, a cutting-edge technique that combines histological imaging with RNA sequencing, to our study. We aim to analyze gene expression levels in a spatially resolved manner, identify new cell types and their spatial distribution, and generate high-resolution maps of gene expression patterns in kidney tissue. To investigate the spatial expression patterns of genes involved in the immune response and other processes affected by APE2-ASO treatment, we propose a study using Visium Spatial Transcriptomics in the APE2 ASO-treated cisplatin-induced AKI model. By adding Visium Spatial Transcriptomics to our ongoing AKI study, we aim to gain a deeper understanding of the cellular and molecular mechanisms involved and identify potential therapeutic targets.